Int. J. Mol. Sci. 2012, 13(9), 11365-11384; doi:10.3390/ijms130911365
Article

Optimization of Ligninolytic Enzyme Activity and Production Rate with Ceriporiopsis subvermispora for Application in Bioremediation by Varying Submerged Media Composition and Growth Immobilization Support

1email, 2email and 1,* email
Received: 21 June 2012; in revised form: 23 August 2012 / Accepted: 5 September 2012 / Published: 12 September 2012
(This article belongs to the Special Issue Enzyme Optimization and Immobilization)
View Full-Text   |   Download PDF [1107 KB, uploaded 19 June 2014]
Abstract: Response surface methodology (central composite design of experiments) was employed to simultaneously optimize enzyme production and productivities of two ligninolytic enzymes produced by Ceriporiopsis subvermispora. Concentrations of glucose, ammonium tartrate and Polysorbate 80 were varied to establish the optimal composition of liquid media (OLM), where the highest experimentally obtained activities and productivities were 41 U L−1 and 16 U L−1 day−1 for laccase (Lac), and 193 U L−1 and 80 U L−1 day−1 for manganese peroxidase (MnP). Considering culture growth in OLM on various types of immobilization support, the best results were obtained with 1 cm beech wood cubes (BWCM). Enzyme activities in culture filtrate were 152 U L−1 for Lac and 58 U L−1 for MnP, since the chemical composition of this immobilization material induced higher Lac activity. Lower enzyme activities were obtained with polyurethane foam. Culture filtrates of OLM and BWCM were applied for dye decolorization. Remazol Brilliant Blue R (RBBR) was decolorized faster and more efficiently than Copper(II)phthalocyanine (CuP) with BWCM (80% and 60%), since Lac played a crucial role. Decolorization of CuP was initially faster than that of RBBR, due to higher MnP activities in OLM. The extent of decolorization after 14 h was 60% for both dyes.
Keywords: laccase; manganese peroxidase; ligninolytic enzymes; enzyme production; medium optimization; submerged cultivation; immobilized culture; bioremediation; response surface methodology; decolorization
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Babič, J.; Likozar, B.; Pavko, A. Optimization of Ligninolytic Enzyme Activity and Production Rate with Ceriporiopsis subvermispora for Application in Bioremediation by Varying Submerged Media Composition and Growth Immobilization Support. Int. J. Mol. Sci. 2012, 13, 11365-11384.

AMA Style

Babič J, Likozar B, Pavko A. Optimization of Ligninolytic Enzyme Activity and Production Rate with Ceriporiopsis subvermispora for Application in Bioremediation by Varying Submerged Media Composition and Growth Immobilization Support. International Journal of Molecular Sciences. 2012; 13(9):11365-11384.

Chicago/Turabian Style

Babič, Janja; Likozar, Blaž; Pavko, Aleksander. 2012. "Optimization of Ligninolytic Enzyme Activity and Production Rate with Ceriporiopsis subvermispora for Application in Bioremediation by Varying Submerged Media Composition and Growth Immobilization Support." Int. J. Mol. Sci. 13, no. 9: 11365-11384.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert