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Combining the Physical Adsorption Approach and the Covalent Attachment Method to Prepare a Bifunctional Bioreactor
Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 30 July 2012; in revised form: 28 August 2012 / Accepted: 5 September 2012 / Published: 12 September 2012
Abstract: Aminopropyl-functionalized SBA-15 mesoporous silica was used as a support to adsorb myoglobin. Then, in order to avoid the leakage of adsorbed myoglobin, lysozyme was covalently tethered to the internal and external surface of the mesoporous silica with glutaraldehyde as the coupling agent. The property of amino-functionalized mesoporous silica was characterized by N2 adsorption-desorption and thermogravimetric (TG) analysis. The feature of the silica-based matrix before and after myoglobin adsorption was identified by fourier transform infrared (FTIR) and UV/VIS measurement. With o-dianisidine and H2O2 as the substrate, the peroxidase activity of adsorbed myoglobin was determined. With Micrococus lysodeilicus as the substrate, the antibacterial activity of covalently tethered lysozyme was measured. Results demonstrated that the final product not only presented peroxidase activity of the myoglobin but yielded antibacterial activity of the lysozyme.
Keywords: adsorption; amino-functionalized mesoporous silica; covalent attachment; myoglobin; lysozyme; peroxidase activity; antibacterial activity
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Dong, M.; Wu, Z.; Lu, M.; Wang, Z.; Li, Z. Combining the Physical Adsorption Approach and the Covalent Attachment Method to Prepare a Bifunctional Bioreactor. Int. J. Mol. Sci. 2012, 13, 11443-11454.
Dong M, Wu Z, Lu M, Wang Z, Li Z. Combining the Physical Adsorption Approach and the Covalent Attachment Method to Prepare a Bifunctional Bioreactor. International Journal of Molecular Sciences. 2012; 13(9):11443-11454.
Dong, Mengxing; Wu, Zhuofu; Lu, Ming; Wang, Zhi; Li, Zhengqiang. 2012. "Combining the Physical Adsorption Approach and the Covalent Attachment Method to Prepare a Bifunctional Bioreactor." Int. J. Mol. Sci. 13, no. 9: 11443-11454.