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Molecular Cloning and Functional Analysis of Three FLOWERING LOCUS T (FT) Homologous Genes from Chinese Cymbidium
Key Laboratory of South China Agricultural Plant Genetics and Breeding, South China Botanical Garden, The Chinese Academy of Sciences, Guangzhou 510650, China
Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
Horticultural Research Institute of Guizhou Province, Guiyang 550006, China
Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan
* Author to whom correspondence should be addressed.
Received: 6 July 2012; in revised form: 11 August 2012 / Accepted: 26 August 2012 / Published: 12 September 2012
Abstract: The FLOWERING LOCUS T (FT) gene plays crucial roles in regulating the transition from the vegetative to reproductive phase. To understand the molecular mechanism of reproduction, three homologous FT genes were isolated and characterized from Cymbidium sinense “Qi Jian Bai Mo”, Cymbidium goeringii and Cymbidium ensifolium “Jin Si Ma Wei”. The three genes contained 618-bp nucleotides with a 531-bp open reading frame (ORF) of encoding 176 amino acids (AAs). Alignment of the AA sequences revealed that CsFT, CgFT and CeFT contain a conserved domain, which is characteristic of the PEBP-RKIP superfamily, and which share high identity with FT of other plants in GenBank: 94% with OnFT from Oncidium Gower Ramsey, 79% with Hd3a from Oryza sativa, and 74% with FT from Arabidopsis thaliana. qRT-PCR analysis showed a diurnal expression pattern of CsFT, CgFT and CeFT following both long day (LD, 16-h light/8-h dark) and short day (SD, 8-h light/16-h dark) treatment. While the transcripts of both CsFT and CeFT under LD were significantly higher than under SD, those of CgFT were higher under SD. Ectopic expression of CgFT in transgenic Arabidopsis plants resulted in early flowering compared to wild-type plants and signiﬁcant up-regulation of APETALA1 (AP1) expression. Our data indicates that CgFT is a putative phosphatidylethanolamine-binding protein gene in Cymbidium that may regulate the vegetative to reproductive transition in ﬂowers, similar to its Arabidopsis ortholog.
Keywords: Cymbidium; FLOWERING LOCUS T; cloning; gene function
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MDPI and ACS Style
Huang, W.; Fang, Z.; Zeng, S.; Zhang, J.; Wu, K.; Chen, Z.; Teixeira da Silva, J.A.; Duan, J. Molecular Cloning and Functional Analysis of Three FLOWERING LOCUS T (FT) Homologous Genes from Chinese Cymbidium. Int. J. Mol. Sci. 2012, 13, 11385-11398.
Huang W, Fang Z, Zeng S, Zhang J, Wu K, Chen Z, Teixeira da Silva JA, Duan J. Molecular Cloning and Functional Analysis of Three FLOWERING LOCUS T (FT) Homologous Genes from Chinese Cymbidium. International Journal of Molecular Sciences. 2012; 13(9):11385-11398.
Huang, Weiting; Fang, Zhongming; Zeng, Songjun; Zhang, Jianxia; Wu, Kunlin; Chen, Zhilin; Teixeira da Silva, Jaime A.; Duan, Jun. 2012. "Molecular Cloning and Functional Analysis of Three FLOWERING LOCUS T (FT) Homologous Genes from Chinese Cymbidium." Int. J. Mol. Sci. 13, no. 9: 11385-11398.