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Int. J. Mol. Sci. 2012, 13(9), 11333-11342; doi:10.3390/ijms130911333
Article

An Imprinted Cross-Linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity

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Received: 28 August 2012; in revised form: 5 September 2012 / Accepted: 5 September 2012 / Published: 11 September 2012
(This article belongs to the Special Issue Enzyme Optimization and Immobilization)
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Abstract: The industrial use of sucrose phosphorylase (SP), an interesting biocatalyst for the selective transfer of α-glucosyl residues to various acceptor molecules, has been hampered by a lack of long-term stability and low activity towards alternative substrates. We have recently shown that the stability of the SP from Bifidobacterium adolescentis can be significantly improved by the formation of a cross-linked enzyme aggregate (CLEA). In this work, it is shown that the transglucosylation activity of such a CLEA can also be improved by molecular imprinting with a suitable substrate. To obtain proof of concept, SP was imprinted with α-glucosyl glycerol and subsequently cross-linked with glutaraldehyde. As a consequence, the enzyme’s specific activity towards glycerol as acceptor substrate was increased two-fold while simultaneously providing an exceptional stability at 60 °C. This procedure can be performed in an aqueous environment and gives rise to a new enzyme formulation called iCLEA.
Keywords: sucrose phosphorylase; immobilization; imprinting; CLEA; glucosyl glycerol sucrose phosphorylase; immobilization; imprinting; CLEA; glucosyl glycerol
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

De Winter, K.; Soetaert, W.; Desmet, T. An Imprinted Cross-Linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity. Int. J. Mol. Sci. 2012, 13, 11333-11342.

AMA Style

De Winter K, Soetaert W, Desmet T. An Imprinted Cross-Linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity. International Journal of Molecular Sciences. 2012; 13(9):11333-11342.

Chicago/Turabian Style

De Winter, Karel; Soetaert, Wim; Desmet, Tom. 2012. "An Imprinted Cross-Linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity." Int. J. Mol. Sci. 13, no. 9: 11333-11342.



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