Int. J. Mol. Sci. 2012, 13(9), 11333-11342; doi:10.3390/ijms130911333
Article

An Imprinted Cross-Linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity

Centre of Expertise for Industrial Biotechnology and Biocatalysis, Department of Biochemical and Microbial Technology, Faculty of Biosciences Engineering, Ghent University, Coupure Links 653, Ghent B-9000, Belgium
* Author to whom correspondence should be addressed.
Received: 28 August 2012; in revised form: 5 September 2012 / Accepted: 5 September 2012 / Published: 11 September 2012
(This article belongs to the Special Issue Enzyme Optimization and Immobilization)
PDF Full-text Download PDF Full-Text [366 KB, uploaded 13 September 2012 17:42 CEST]
Abstract: The industrial use of sucrose phosphorylase (SP), an interesting biocatalyst for the selective transfer of α-glucosyl residues to various acceptor molecules, has been hampered by a lack of long-term stability and low activity towards alternative substrates. We have recently shown that the stability of the SP from Bifidobacterium adolescentis can be significantly improved by the formation of a cross-linked enzyme aggregate (CLEA). In this work, it is shown that the transglucosylation activity of such a CLEA can also be improved by molecular imprinting with a suitable substrate. To obtain proof of concept, SP was imprinted with α-glucosyl glycerol and subsequently cross-linked with glutaraldehyde. As a consequence, the enzyme’s specific activity towards glycerol as acceptor substrate was increased two-fold while simultaneously providing an exceptional stability at 60 °C. This procedure can be performed in an aqueous environment and gives rise to a new enzyme formulation called iCLEA.
Keywords: sucrose phosphorylase; immobilization; imprinting; CLEA; glucosyl glycerol

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

De Winter, K.; Soetaert, W.; Desmet, T. An Imprinted Cross-Linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity. Int. J. Mol. Sci. 2012, 13, 11333-11342.

AMA Style

De Winter K, Soetaert W, Desmet T. An Imprinted Cross-Linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity. International Journal of Molecular Sciences. 2012; 13(9):11333-11342.

Chicago/Turabian Style

De Winter, Karel; Soetaert, Wim; Desmet, Tom. 2012. "An Imprinted Cross-Linked Enzyme Aggregate (iCLEA) of Sucrose Phosphorylase: Combining Improved Stability with Altered Specificity." Int. J. Mol. Sci. 13, no. 9: 11333-11342.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert