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Isolation and Characterization of Microsatellite Markers for Passiflora contracta
Laboratory of Molecular Evolution, Department of Genetics, Federal University of Rio Grande do Sul, PO Box 15053, 91501-970 Porto Alegre, RS, Brazil
Molecular Ecology Laboratory, School of Biological Sciences Flinders University, GPO Box 2100, Adelaide 5001, Australia
Laboratory of Genomic and Molecular Biology, Pontifical Catholic University of Rio Grande do Sul, Ipiranga 6681, 90610-001 Porto Alegre, RS, Brazil
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 27 August 2012; in revised form: 7 September 2012 / Accepted: 8 September 2012 / Published: 12 September 2012
Abstract: Passiflora contracta Vitta (Passifloraceae) is an endemic species of the Atlantic Rainforest, one of the most species-rich ecoregions in the world, although extremely endangered. We have developed an enriched microsatellite library in order to fine-scale studies of the genetic structure of P. contracta. Twelve pairs of microsatellite primers were designed, and seven loci were successfully amplified and characterized by genotyping two wild populations of P. contracta. All seven loci were polymorphic, with an average number of alleles found being 4.8 and 5 per population. The cross-species transferability was tested using sister species Passiflora ovalis Vell. Ex Roemer. The development of these markers will contribute to the studies of population genetics in P. contracta as well as future studies concerning diversity patterns in the Atlantic Rainforest, and may also help to establish strategies for the conservation of this species.
Keywords: nuclear microsatellites; Passiflora contracta; conservation genetics
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Cazé, A.L.R.; Kriedt, R.A.; Beheregaray, L.B.; Bonatto, S.L.; Freitas, L.B. Isolation and Characterization of Microsatellite Markers for Passiflora contracta. Int. J. Mol. Sci. 2012, 13, 11343-11348.
Cazé ALR, Kriedt RA, Beheregaray LB, Bonatto SL, Freitas LB. Isolation and Characterization of Microsatellite Markers for Passiflora contracta. International Journal of Molecular Sciences. 2012; 13(9):11343-11348.
Cazé, Ana Luíza R.; Kriedt, Raquel A.; Beheregaray, Luciano B.; Bonatto, Sandro L.; Freitas, Loreta B. 2012. "Isolation and Characterization of Microsatellite Markers for Passiflora contracta." Int. J. Mol. Sci. 13, no. 9: 11343-11348.