Int. J. Mol. Sci. 2012, 13(8), 10630-10646; doi:10.3390/ijms130810630
Article

Optimization of Xylanase Production from Penicillium sp.WX-Z1 by a Two-Step Statistical Strategy: Plackett-Burman and Box-Behnken Experimental Design

1 School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China 2 Department of Respiratory Disease, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200003, China
* Author to whom correspondence should be addressed.
Received: 6 June 2012; in revised form: 23 July 2012 / Accepted: 2 August 2012 / Published: 23 August 2012
(This article belongs to the Special Issue Enzyme Optimization and Immobilization)
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Abstract: The objective of the study was to optimize the nutrition sources in a culture medium for the production of xylanase from Penicillium sp.WX-Z1 using Plackett-Burman design and Box-Behnken design. The Plackett-Burman multifactorial design was first employed to screen the important nutrient sources in the medium for xylanase production by Penicillium sp.WX-Z1 and subsequent use of the response surface methodology (RSM) was further optimized for xylanase production by Box-Behnken design. The important nutrient sources in the culture medium, identified by the initial screening method of Placket-Burman, were wheat bran, yeast extract, NaNO3, MgSO4, and CaCl2. The optimal amounts (in g/L) for maximum production of xylanase were: wheat bran, 32.8; yeast extract, 1.02; NaNO3, 12.71; MgSO4, 0.96; and CaCl2, 1.04. Using this statistical experimental design, the xylanase production under optimal condition reached 46.50 U/mL and an increase in xylanase activity of 1.34-fold was obtained compared with the original medium for fermentation carried out in a 30-L bioreactor.
Keywords: Penicillium sp.WX-Z1; xylanase; nutrient sources; Plackett-Burman design; Box-Behnken design

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MDPI and ACS Style

Cui, F.; Zhao, L. Optimization of Xylanase Production from Penicillium sp.WX-Z1 by a Two-Step Statistical Strategy: Plackett-Burman and Box-Behnken Experimental Design. Int. J. Mol. Sci. 2012, 13, 10630-10646.

AMA Style

Cui F, Zhao L. Optimization of Xylanase Production from Penicillium sp.WX-Z1 by a Two-Step Statistical Strategy: Plackett-Burman and Box-Behnken Experimental Design. International Journal of Molecular Sciences. 2012; 13(8):10630-10646.

Chicago/Turabian Style

Cui, Fengjie; Zhao, Liming. 2012. "Optimization of Xylanase Production from Penicillium sp.WX-Z1 by a Two-Step Statistical Strategy: Plackett-Burman and Box-Behnken Experimental Design." Int. J. Mol. Sci. 13, no. 8: 10630-10646.

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