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Int. J. Mol. Sci. 2012, 13(8), 10630-10646; doi:10.3390/ijms130810630
Article

Optimization of Xylanase Production from Penicillium sp.WX-Z1 by a Two-Step Statistical Strategy: Plackett-Burman and Box-Behnken Experimental Design

1
 and 2,*
Received: 6 June 2012; in revised form: 23 July 2012 / Accepted: 2 August 2012 / Published: 23 August 2012
(This article belongs to the Special Issue Enzyme Optimization and Immobilization)
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Abstract: The objective of the study was to optimize the nutrition sources in a culture medium for the production of xylanase from Penicillium sp.WX-Z1 using Plackett-Burman design and Box-Behnken design. The Plackett-Burman multifactorial design was first employed to screen the important nutrient sources in the medium for xylanase production by Penicillium sp.WX-Z1 and subsequent use of the response surface methodology (RSM) was further optimized for xylanase production by Box-Behnken design. The important nutrient sources in the culture medium, identified by the initial screening method of Placket-Burman, were wheat bran, yeast extract, NaNO3, MgSO4, and CaCl2. The optimal amounts (in g/L) for maximum production of xylanase were: wheat bran, 32.8; yeast extract, 1.02; NaNO3, 12.71; MgSO4, 0.96; and CaCl2, 1.04. Using this statistical experimental design, the xylanase production under optimal condition reached 46.50 U/mL and an increase in xylanase activity of 1.34-fold was obtained compared with the original medium for fermentation carried out in a 30-L bioreactor.
Keywords: Penicillium sp.WX-Z1; xylanase; nutrient sources; Plackett-Burman design; Box-Behnken design Penicillium sp.WX-Z1; xylanase; nutrient sources; Plackett-Burman design; Box-Behnken design
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Cui, F.; Zhao, L. Optimization of Xylanase Production from Penicillium sp.WX-Z1 by a Two-Step Statistical Strategy: Plackett-Burman and Box-Behnken Experimental Design. Int. J. Mol. Sci. 2012, 13, 10630-10646.

AMA Style

Cui F, Zhao L. Optimization of Xylanase Production from Penicillium sp.WX-Z1 by a Two-Step Statistical Strategy: Plackett-Burman and Box-Behnken Experimental Design. International Journal of Molecular Sciences. 2012; 13(8):10630-10646.

Chicago/Turabian Style

Cui, Fengjie; Zhao, Liming. 2012. "Optimization of Xylanase Production from Penicillium sp.WX-Z1 by a Two-Step Statistical Strategy: Plackett-Burman and Box-Behnken Experimental Design." Int. J. Mol. Sci. 13, no. 8: 10630-10646.


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