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Tissue Microarray-Based Evaluation of Chromatin Assembly Factor-1 (CAF-1)/p60 as Tumour Prognostic Marker
Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples “Federico II”, Naples 80138, Italy
* Author to whom correspondence should be addressed.
Received: 18 June 2012; in revised form: 22 August 2012 / Accepted: 23 August 2012 / Published: 5 September 2012
Abstract: In this study we aimed to confirm the emerging role of Chromatin Assembly Factor 1 (CAF-1 p60) as a new proliferation and prognostic marker for cancer and to test the usefulness of the tissue microarray technique (TMA) for CAF-1 p60 rapid screening in several human malignancies. CAF-1 is a histone chaperone, regulating chromatin dynamics during DNA replication and repair in eukaryotics. TMA is a powerful high-throughput methodology in the study of cancer, allowing simultaneous assessment of different biomarkers within large numbers of tissue specimens. We generated TMA taking 3 mm diameter-core biopsies from oral squamous cell carcinoma, prostate cancer, salivary gland tumours and skin melanoma specimens, which had been previously tested for CAF-1 p60 on routine tissue sections. We also analysed, for the first time, 30 larynx and 30 skin squamous cell carcinomas. CAF-1 p60 resulted over-expressed in both the tissue sections and the TMA specimens, with the highest levels of expression in tumours which were more aggressive and metastasizing. Notably, a high degree of agreement was found between the CAF-1 p60 assessment on TMAs and on routine tissue sections. Our findings confirm the prognostic role of CAF-1 p60 and indicate TMA as a really advantageous method for CAF-1 p60 immunohistochemical screening, allowing savings on both tissue quantity and operator-time.
Keywords: Chromatin Assembly Factor-1; tissue microarray; immunohistochemistry; cancer screening
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Mascolo, M.; Ilardi, G.; Merolla, F.; Russo, D.; Vecchione, M.L.; de Rosa, G.; Staibano, S. Tissue Microarray-Based Evaluation of Chromatin Assembly Factor-1 (CAF-1)/p60 as Tumour Prognostic Marker. Int. J. Mol. Sci. 2012, 13, 11044-11062.
Mascolo M, Ilardi G, Merolla F, Russo D, Vecchione ML, de Rosa G, Staibano S. Tissue Microarray-Based Evaluation of Chromatin Assembly Factor-1 (CAF-1)/p60 as Tumour Prognostic Marker. International Journal of Molecular Sciences. 2012; 13(9):11044-11062.
Mascolo, Massimo; Ilardi, Gennaro; Merolla, Francesco; Russo, Daniela; Vecchione, Maria Luisa; de Rosa, Gaetano; Staibano, Stefania. 2012. "Tissue Microarray-Based Evaluation of Chromatin Assembly Factor-1 (CAF-1)/p60 as Tumour Prognostic Marker." Int. J. Mol. Sci. 13, no. 9: 11044-11062.