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Int. J. Mol. Sci. 2012, 13(9), 11044-11062; doi:10.3390/ijms130911044
Article

Tissue Microarray-Based Evaluation of Chromatin Assembly Factor-1 (CAF-1)/p60 as Tumour Prognostic Marker

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Received: 18 June 2012 / Revised: 22 August 2012 / Accepted: 23 August 2012 / Published: 5 September 2012
(This article belongs to the Special Issue Advances in Molecular Oncology (special issue))
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Abstract

In this study we aimed to confirm the emerging role of Chromatin Assembly Factor 1 (CAF-1 p60) as a new proliferation and prognostic marker for cancer and to test the usefulness of the tissue microarray technique (TMA) for CAF-1 p60 rapid screening in several human malignancies. CAF-1 is a histone chaperone, regulating chromatin dynamics during DNA replication and repair in eukaryotics. TMA is a powerful high-throughput methodology in the study of cancer, allowing simultaneous assessment of different biomarkers within large numbers of tissue specimens. We generated TMA taking 3 mm diameter-core biopsies from oral squamous cell carcinoma, prostate cancer, salivary gland tumours and skin melanoma specimens, which had been previously tested for CAF-1 p60 on routine tissue sections. We also analysed, for the first time, 30 larynx and 30 skin squamous cell carcinomas. CAF-1 p60 resulted over-expressed in both the tissue sections and the TMA specimens, with the highest levels of expression in tumours which were more aggressive and metastasizing. Notably, a high degree of agreement was found between the CAF-1 p60 assessment on TMAs and on routine tissue sections. Our findings confirm the prognostic role of CAF-1 p60 and indicate TMA as a really advantageous method for CAF-1 p60 immunohistochemical screening, allowing savings on both tissue quantity and operator-time.
Keywords: Chromatin Assembly Factor-1; tissue microarray; immunohistochemistry; cancer screening Chromatin Assembly Factor-1; tissue microarray; immunohistochemistry; cancer screening
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mascolo, M.; Ilardi, G.; Merolla, F.; Russo, D.; Vecchione, M.L.; de Rosa, G.; Staibano, S. Tissue Microarray-Based Evaluation of Chromatin Assembly Factor-1 (CAF-1)/p60 as Tumour Prognostic Marker. Int. J. Mol. Sci. 2012, 13, 11044-11062.

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