Int. J. Mol. Sci. 2012, 13(7), 9207-9217; doi:10.3390/ijms13079207
Article

3D Structure Elucidation of Thermostable L2 Lipase from Thermophilic Bacillus sp. L2

1 Faculty of Biotechnology and Biomolecular Sciences, Enzyme and Microbial Technology Research, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia 2 Institute of Bioscience, Enzyme and Microbial Technology Research, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia 3 Faculty of Science, Enzyme and Microbial Technology Research, Universiti Putra Malaysia, UPM Serdang, Selangor 43400, Malaysia
* Author to whom correspondence should be addressed.
Received: 16 May 2012; in revised form: 29 June 2012 / Accepted: 12 July 2012 / Published: 23 July 2012
(This article belongs to the Special Issue Protein Crystallography in Molecular Biology)
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Abstract: The crystallization of proteins makes it possible to determine their structure by X-ray crystallography, and is therefore important for the analysis of protein structure-function relationships. L2 lipase was crystallized by using the J-tube counter diffusion method. A crystallization consisting of 20% PEG 6000, 50 mM MES pH 6.5 and 50 mM NaCl was found to be the best condition to produce crystals with good shape and size (0.5 × 0.1 × 0.2 mm). The protein concentration used for the crystallization was 3 mg/mL. L2 lipase crystal has two crystal forms, Shape 1 and Shape 2. Shape 2 L2 lipase crystal was diffracted at 1.5 Å and the crystal belongs to the orthorhombic space group P212121, with unit-cell parameters a = 72.0, b = 81.8, c = 83.4 Å, α = β = γ = 90°. There is one molecule per asymmetric unit and the solvent content of the crystals is 56.9%, with a Matthew’s coefficient of 2.85 Å Da−1. The 3D structure of L2 lipase revealed topological organization of α/β-hydrolase fold consisting of 11 β-strands and 13 α-helices. Ser-113, His-358 and Asp-317 were assigned as catalytic triad residues. One Ca2+ and one Zn2+ were found in the L2 lipase molecule.
Keywords: thermostable lipase; L2 lipase; crystallization; counterdiffusion; crystal structure

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MDPI and ACS Style

Raja Abd. Rahman, R.N.Z.; Mohd Shariff, F.; Basri, M.; Salleh, A.B. 3D Structure Elucidation of Thermostable L2 Lipase from Thermophilic Bacillus sp. L2. Int. J. Mol. Sci. 2012, 13, 9207-9217.

AMA Style

Raja Abd. Rahman RNZ, Mohd Shariff F, Basri M, Salleh AB. 3D Structure Elucidation of Thermostable L2 Lipase from Thermophilic Bacillus sp. L2. International Journal of Molecular Sciences. 2012; 13(7):9207-9217.

Chicago/Turabian Style

Raja Abd. Rahman, Raja Noor Zaliha; Mohd Shariff, Fairolniza; Basri, Mahiran; Salleh, Abu Bakar. 2012. "3D Structure Elucidation of Thermostable L2 Lipase from Thermophilic Bacillus sp. L2." Int. J. Mol. Sci. 13, no. 7: 9207-9217.

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