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Int. J. Mol. Sci. 2012, 13(7), 9218-9224; doi:10.3390/ijms13079218
Short Note

Development of 20 Microsatellite Markers for Solenocera crassicornis and Their Cross-Species Application in Solenocera melantho

1
, 1,2
, 3
, 1,4
, 1
 and 1,*
1 Key Laboratory for Fishery Resources and Eco-Environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China 2 Key Laboratory of Mariculture, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China 3 South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China 4 College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
* Author to whom correspondence should be addressed.
Received: 28 June 2012 / Revised: 11 July 2012 / Accepted: 17 July 2012 / Published: 23 July 2012
(This article belongs to the Section Molecular Pathology)
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Abstract

Twenty microsatellite markers were isolated and characterized for Solenocera crassicornis from a (GT)13-enriched genomic library. Their polymorphisms were investigated using 44 wild individuals from the South Yellow Sea. Our investigation revealed that all the markers were polymorphic. The number of alleles per locus varied from 6 to 19 with an average of 12.35. The observed and expected heterozygosities ranged from 0.400 to 0.977 and from 0.609 to 0.940, with averages of 0.788 and 0.859, respectively. Four loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni’s correction. Cross-species amplification was also conducted in Solenocera melantho collected from the East China Sea. The result showed that 14 loci could be amplified from Solenocera melantho DNAs. These polymorphic markers would be useful for assessment of genetic variation and population structure of S. crassicornis and S. melantho.
Keywords: Solenocera crassicornis; Solenocera melantho; microsatellite marker; cross-species amplification Solenocera crassicornis; Solenocera melantho; microsatellite marker; cross-species amplification
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Liu, S.; Liu, H.; Lin, L.; Yuan, Y.; Dai, F.; Zhuang, Z. Development of 20 Microsatellite Markers for Solenocera crassicornis and Their Cross-Species Application in Solenocera melantho. Int. J. Mol. Sci. 2012, 13, 9218-9224.

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