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Development of 20 Microsatellite Markers for Solenocera crassicornis and Their Cross-Species Application in Solenocera melantho
Key Laboratory for Fishery Resources and Eco-Environment, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
Key Laboratory of Mariculture, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
* Author to whom correspondence should be addressed.
Received: 28 June 2012; in revised form: 11 July 2012 / Accepted: 17 July 2012 / Published: 23 July 2012
Abstract: Twenty microsatellite markers were isolated and characterized for Solenocera crassicornis from a (GT)13-enriched genomic library. Their polymorphisms were investigated using 44 wild individuals from the South Yellow Sea. Our investigation revealed that all the markers were polymorphic. The number of alleles per locus varied from 6 to 19 with an average of 12.35. The observed and expected heterozygosities ranged from 0.400 to 0.977 and from 0.609 to 0.940, with averages of 0.788 and 0.859, respectively. Four loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni’s correction. Cross-species amplification was also conducted in Solenocera melantho collected from the East China Sea. The result showed that 14 loci could be amplified from Solenocera melantho DNAs. These polymorphic markers would be useful for assessment of genetic variation and population structure of S. crassicornis and S. melantho.
Keywords: Solenocera crassicornis; Solenocera melantho; microsatellite marker; cross-species amplification
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MDPI and ACS Style
Liu, S.; Liu, H.; Lin, L.; Yuan, Y.; Dai, F.; Zhuang, Z. Development of 20 Microsatellite Markers for Solenocera crassicornis and Their Cross-Species Application in Solenocera melantho. Int. J. Mol. Sci. 2012, 13, 9218-9224.
Liu S, Liu H, Lin L, Yuan Y, Dai F, Zhuang Z. Development of 20 Microsatellite Markers for Solenocera crassicornis and Their Cross-Species Application in Solenocera melantho. International Journal of Molecular Sciences. 2012; 13(7):9218-9224.
Liu, Shufang; Liu, Hongbo; Lin, Lin; Yuan, Yanjiao; Dai, Fangqun; Zhuang, Zhimeng. 2012. "Development of 20 Microsatellite Markers for Solenocera crassicornis and Their Cross-Species Application in Solenocera melantho." Int. J. Mol. Sci. 13, no. 7: 9218-9224.