Int. J. Mol. Sci. 2012, 13(7), 9194-9206; doi:10.3390/ijms13079194
Article

Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus

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Received: 22 May 2012; in revised form: 4 July 2012 / Accepted: 16 July 2012 / Published: 23 July 2012
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Abstract: The mushroom Inonotus obliquus has been widely used as a folk medicine in Russia, Poland and most of the Baltic countries. In this study, water-soluble and alkali-soluble crude polysaccharides (IOW and IOA) were isolated from I. obliquus, and the carbohydrate-rich fractions IOW-1 and IOA-1 were obtained respectively after deproteination and depigmentation. Their contents, such as neutral carbohydrate, uronic acid and protein, were measured. Their antioxidant properties against chemicals-induced reactive species (ROS) including 1,1'-Diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide anion radical, as well as their protective effects on H2O2-induced PC12 cell death were investigated. Results showed that I. obliquus polysaccharides can scavenge all ROS tested above in a dose-dependent manner. IOA and its product IOA-1 could rescue PC12 cell viability from 38.6% to 79.8% and 83.0% at a concentration of 20µg/mL. Similarly, IOW and its product IOW-1 at the same dose, can also increase cell viability to 84.9% and 88.6% respectively. The antioxidative activities of water-soluble and alkali-soluble polysaccharide constituents from I. obliquus might contribute to diverse medicinal and nutritional values of this mushroom.
Keywords: Inonotus obliquus; polysaccharide; antioxidant; reactive oxygen species
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Mu, H.; Zhang, A.; Zhang, W.; Cui, G.; Wang, S.; Duan, J. Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus. Int. J. Mol. Sci. 2012, 13, 9194-9206.

AMA Style

Mu H, Zhang A, Zhang W, Cui G, Wang S, Duan J. Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus. International Journal of Molecular Sciences. 2012; 13(7):9194-9206.

Chicago/Turabian Style

Mu, Haibo; Zhang, Amin; Zhang, Wuxia; Cui, Guoting; Wang, Shunchun; Duan, Jinyou. 2012. "Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus." Int. J. Mol. Sci. 13, no. 7: 9194-9206.


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