Abstract: The mushroom Inonotus obliquus has been widely used as a folk medicine in Russia, Poland and most of the Baltic countries. In this study, water-soluble and alkali-soluble crude polysaccharides (IOW and IOA) were isolated from I. obliquus, and the carbohydrate-rich fractions IOW-1 and IOA-1 were obtained respectively after deproteination and depigmentation. Their contents, such as neutral carbohydrate, uronic acid and protein, were measured. Their antioxidant properties against chemicals-induced reactive species (ROS) including 1,1'-Diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide anion radical, as well as their protective effects on H2O2-induced PC12 cell death were investigated. Results showed that I. obliquus polysaccharides can scavenge all ROS tested above in a dose-dependent manner. IOA and its product IOA-1 could rescue PC12 cell viability from 38.6% to 79.8% and 83.0% at a concentration of 20µg/mL. Similarly, IOW and its product IOW-1 at the same dose, can also increase cell viability to 84.9% and 88.6% respectively. The antioxidative activities of water-soluble and alkali-soluble polysaccharide constituents from I. obliquus might contribute to diverse medicinal and nutritional values of this mushroom.
Keywords: Inonotus obliquus; polysaccharide; antioxidant; reactive oxygen species
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Mu, H.; Zhang, A.; Zhang, W.; Cui, G.; Wang, S.; Duan, J. Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus. Int. J. Mol. Sci. 2012, 13, 9194-9206.
Mu H, Zhang A, Zhang W, Cui G, Wang S, Duan J. Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus. International Journal of Molecular Sciences. 2012; 13(7):9194-9206.
Mu, Haibo; Zhang, Amin; Zhang, Wuxia; Cui, Guoting; Wang, Shunchun; Duan, Jinyou. 2012. "Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus." Int. J. Mol. Sci. 13, no. 7: 9194-9206.