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Int. J. Mol. Sci. 2012, 13(7), 9194-9206; doi:10.3390/ijms13079194
Article

Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus

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1 College of Science, Northwest A&F University, Yangling, Shaanxi 712100, China 2 Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
* Authors to whom correspondence should be addressed.
Received: 22 May 2012 / Revised: 4 July 2012 / Accepted: 16 July 2012 / Published: 23 July 2012
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Abstract

The mushroom Inonotus obliquus has been widely used as a folk medicine in Russia, Poland and most of the Baltic countries. In this study, water-soluble and alkali-soluble crude polysaccharides (IOW and IOA) were isolated from I. obliquus, and the carbohydrate-rich fractions IOW-1 and IOA-1 were obtained respectively after deproteination and depigmentation. Their contents, such as neutral carbohydrate, uronic acid and protein, were measured. Their antioxidant properties against chemicals-induced reactive species (ROS) including 1,1'-Diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide anion radical, as well as their protective effects on H2O2-induced PC12 cell death were investigated. Results showed that I. obliquus polysaccharides can scavenge all ROS tested above in a dose-dependent manner. IOA and its product IOA-1 could rescue PC12 cell viability from 38.6% to 79.8% and 83.0% at a concentration of 20µg/mL. Similarly, IOW and its product IOW-1 at the same dose, can also increase cell viability to 84.9% and 88.6% respectively. The antioxidative activities of water-soluble and alkali-soluble polysaccharide constituents from I. obliquus might contribute to diverse medicinal and nutritional values of this mushroom.
Keywords: Inonotus obliquus; polysaccharide; antioxidant; reactive oxygen species Inonotus obliquus; polysaccharide; antioxidant; reactive oxygen species
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Mu, H.; Zhang, A.; Zhang, W.; Cui, G.; Wang, S.; Duan, J. Antioxidative Properties of Crude Polysaccharides from Inonotus obliquus. Int. J. Mol. Sci. 2012, 13, 9194-9206.

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