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Int. J. Mol. Sci. 2012, 13(12), 16986-17005; doi:10.3390/ijms131216986
Review

Intelligent Design of Nano-Scale Molecular Imaging Agents

1,* , 2
 and 2
Received: 21 September 2012; Accepted: 26 November 2012 / Published: 12 December 2012
(This article belongs to the Special Issue Bioactive Nanoparticles 2012)
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Abstract: Visual representation and quantification of biological processes at the cellular and subcellular levels within living subjects are gaining great interest in life science to address frontier issues in pathology and physiology. As intact living subjects do not emit any optical signature, visual representation usually exploits nano-scale imaging agents as the source of image contrast. Many imaging agents have been developed for this purpose, some of which exert nonspecific, passive, and physical interaction with a target. Current research interest in molecular imaging has mainly shifted to fabrication of smartly integrated, specific, and versatile agents that emit fluorescence or luminescence as an optical readout. These agents include luminescent quantum dots (QDs), biofunctional antibodies, and multifunctional nanoparticles. Furthermore, genetically encoded nano-imaging agents embedding fluorescent proteins or luciferases are now gaining popularity. These agents are generated by integrative design of the components, such as luciferase, flexible linker, and receptor to exert a specific on–off switching in the complex context of living subjects. In the present review, we provide an overview of the basic concepts, smart design, and practical contribution of recent nano-scale imaging agents, especially with respect to genetically encoded imaging agents.
Keywords: imaging agent; molecular probe; optical signature; smart design; bioluminescence imaging agent; molecular probe; optical signature; smart design; bioluminescence
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kim, S.B.; Hattori, M.; Ozawa, T. Intelligent Design of Nano-Scale Molecular Imaging Agents. Int. J. Mol. Sci. 2012, 13, 16986-17005.

AMA Style

Kim SB, Hattori M, Ozawa T. Intelligent Design of Nano-Scale Molecular Imaging Agents. International Journal of Molecular Sciences. 2012; 13(12):16986-17005.

Chicago/Turabian Style

Kim, Sung B.; Hattori, Mitsuru; Ozawa, Takeaki. 2012. "Intelligent Design of Nano-Scale Molecular Imaging Agents." Int. J. Mol. Sci. 13, no. 12: 16986-17005.



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