Abstract: Fluorescent silica nanoparticles (FSNPs) can provide high-intensity and photostable fluorescent signals as a probe for biomedical analysis. In this study, FSNPs hybridized with aggregation-induced emission (AIE) luminogens (namely FSNP-SD) were successfully fabricated by a surfactant-free sol-gel method. The FSNP-SD were spherical, monodisperse and uniform in size, with an average diameter of approximately 100 nm, and emitted strong fluorescence at the peak of 490 nm. The FSNP-SD selectively stained the cytoplasmic regions and were distributed in the cytoplasm. Moreover, they can stay inside cells, enabling the tacking of cells over a long period of time. The intracellular vesicles and multinucleated cells were increase gradually with the rise of FSNP-SD concentration. Both cell viability and survival only lost less than 20% when the cells were exposed to the high concentration of 100 μg/mL FSNP-SD. Additionally, the cell apoptosis and intracellular ROS assay indicated that FSNP-SD had no significant toxic effects at the maximum working concentration of 80 μg/mL. This study demonstrated that the FSNP-SD are promising biocompatible fluorescent probes for living cell imaging.
Keywords: aggregation-induced emission; fluorescent silica nanoparticles; cytotoxicity; cell imaging; silole
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Xia, Y.; Li, M.; Peng, T.; Zhang, W.; Xiong, J.; Hu, Q.; Song, Z.; Zheng, Q. In Vitro Cytotoxicity of Fluorescent Silica Nanoparticles Hybridized with Aggregation-Induced Emission Luminogens for Living Cell Imaging. Int. J. Mol. Sci. 2013, 14, 1080-1092.
Xia Y, Li M, Peng T, Zhang W, Xiong J, Hu Q, Song Z, Zheng Q. In Vitro Cytotoxicity of Fluorescent Silica Nanoparticles Hybridized with Aggregation-Induced Emission Luminogens for Living Cell Imaging. International Journal of Molecular Sciences. 2013; 14(1):1080-1092.
Xia, Yun; Li, Min; Peng, Tao; Zhang, Weijie; Xiong, Jun; Hu, Qinggang; Song, Zifang; Zheng, Qichang. 2013. "In Vitro Cytotoxicity of Fluorescent Silica Nanoparticles Hybridized with Aggregation-Induced Emission Luminogens for Living Cell Imaging." Int. J. Mol. Sci. 14, no. 1: 1080-1092.