Int. J. Mol. Sci. 2012, 13(12), 16592-16597; doi:10.3390/ijms131216592
Short Note

Isolation and Characterization of Microsatellite Markers in the Domestic Ferret (Mustela putorius furo)

1,2,* email, 3email, 1email and 3email
Received: 25 September 2012; in revised form: 22 October 2012 / Accepted: 23 November 2012 / Published: 5 December 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: The domestic ferret (Mustela putorius furo) is an important model organism for the study of avian influenza and other diseases of humans and animals, as well as a popular pet animal. In order to evaluate genetic diversity and study disease relationships in ferrets, 22 nuclear microsatellite loci (17 dinucleotide and 5 tetranucleotide) were developed from ferret genomic libraries and organized into seven multiplex sets. Polymorphism was preliminarily assessed in one population in Australia and one in the USA, sampled with 25 individuals each. The loci displayed allelic diversity ranging from 1 to 5 alleles, and expected and observed heterozygosities ranging from 0.04 to 0.65 and 0.04 to 0.76, respectively. Additionally, the loci amplified products in 15 samples from the wild ancestor, European polecat (Mustela putorius) and domestic ferret-polecat hybrids. In polecat/hybrid samples, allelic diversity ranged from 3 to 8 alleles, and expected and observed heterozygosities ranged from 0.13 to 0.81 and 0.13 to 0.80 respectively. These markers will be useful for molecular assessments of genetic diversity and applications to evolution, ecology, and health in domestic ferrets and wild polecats.
Keywords: Mustela putorius furo; domestic ferret; microsatellite; polymorphism; genetic diversity; multiplex panel
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ernest, H.B.; Drazenovich, T.L.; Dalbeck, L.S.; Hawkins, M.G. Isolation and Characterization of Microsatellite Markers in the Domestic Ferret (Mustela putorius furo). Int. J. Mol. Sci. 2012, 13, 16592-16597.

AMA Style

Ernest HB, Drazenovich TL, Dalbeck LS, Hawkins MG. Isolation and Characterization of Microsatellite Markers in the Domestic Ferret (Mustela putorius furo). International Journal of Molecular Sciences. 2012; 13(12):16592-16597.

Chicago/Turabian Style

Ernest, Holly B.; Drazenovich, Tracy L.; Dalbeck, Lisa S.; Hawkins, Michelle G. 2012. "Isolation and Characterization of Microsatellite Markers in the Domestic Ferret (Mustela putorius furo)." Int. J. Mol. Sci. 13, no. 12: 16592-16597.


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