• Abstract
• Full-Text PDF [1358 KB]
• Full-Text XML
• Article Versions Notes
• Supplement File 1
• Supplement File 2
• Supplement File 3
• Supplement File 4

• Article Statistics
• PubMed/Medline
• Google Scholar
• Order Reprints

• Cite this article
• Export to BibTeX
• Export to EndNote

• [+] on DOAJ
• Yang, L
• Tong, M
• Chi, X
• Zhang, M
• Zhang, C
• Guo, X
• [+] on Google Scholar
• Yang, L
• Tong, M
• Chi, X
• Zhang, M
• Zhang, C
• Guo, X
• [+] on PubMed
• Yang, L
• Tong, M
• Chi, X
• Zhang, M
• Zhang, C
• Guo, X

•  CiteULike
•  Facebook
•  Mendeley
•  Twitter

This article is

• freely available
• re-usable

Int. J. Mol. Sci. 2012, 13(12), 15575-15587; doi:10.3390/ijms131215575

Article

Genomic DNA Methylation Changes in NYGGF4-Overexpression 3T3-L1 Adipocytes

Lei Yang ^1,2 , Mei-Ling Tong ¹ , Xia Chi ¹ , Min Zhang ¹ , Chun-Mei Zhang ^1,*  and Xi-Rong Guo ^1,2,*

¹ State Key Laboratory of Reproductive Medicine, Department of Pediatrics, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, No 123 Tianfei Xiang, Mochou Road, Nanjing 210029, China ² Institute of Pediatrics, Nanjing Medical University, No.140 Hanzhong Road, Nanjing 210029, China

* Authors to whom correspondence should be addressed.

Received: 19 September 2012; in revised form: 6 November 2012 / Accepted: 16 November 2012 / Published: 22 November 2012

(This article belongs to the Section Molecular Pathology)

Download PDF Full-Text [1358 KB, uploaded 22 November 2012 15:34 CET]

Abstract: NYGGF4, an obesity-related gene, is proposed to be involved in the development of insulin resistance; however, the underlying molecular mechanisms remain unclear. In the present analysis, NimbleGen tiling arrays were used to determine the patterns of genomic DNA methylation at CpG islands and promoters in NYGGF4-overexpression adipocytes. A total of 2352 CpG dinucleotides in 2018 genes and 3490 CpG dinucleotides in 3064 genes were found to be hypermethylated or hypomethylated, respectively, in NYGGF4-overexpression adipocytes. Furthermore, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis revealed enrichment of biological processes associated with energy metabolism and signal transduction events, including the peroxisome proliferator-activated receptor gamma (PPARγ) signaling pathway, and mitogen-activated protein kinases(MAPK) and Ras homolog gene family, member A (RhoA) signaling. These data demonstrate that differentially methylated genes are significantly overrepresented in NYGGF4-overexpression adipocytes, providing valuable clues for further exploration of the role of NYGGF4 in insulin sensitivity regulation.

Keywords: NYGGF4; DNA methylation; insulin resistance; adipocytes

Supplementary Files

• Supplementary File 1:

  Table 1 (XLS, 103 KB)

• Supplementary File 2:

  Table 2 (XLS, 57 KB)

• Supplementary File 3:

  Table 3 (XLS, 45 KB)

• Supplementary File 4:

  Table 4 (XLS, 49 KB)

Article Statistics

Cite This Article