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• Luo, M
• Ding, L
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• Sun, Q
• Xu, Z
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• Ding, L
• Ge, Z
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• Hu, B
• Yang, X
• Sun, Q
• Xu, Z
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• Ding, L
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Int. J. Mol. Sci. 2012, 13(11), 15162-15176; doi:10.3390/ijms131115162

Article

The Characterization of SaPIN2b, a Plant Trichome-Localized Proteinase Inhibitor from Solanum americanum

Ming Luo ^1,2,† , Ling-Wen Ding ^1,† , Zhi-Juan Ge ^1,† , Zhen-Yu Wang ¹ , Bo-Lun Hu ¹ , Xiao-Bei Yang ¹ , Qiao-Yang Sun ¹  and Zeng-Fu Xu ^1,3,*

¹ State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China ² Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China ³ Key Laboratory of Tropical Plant Resource and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Yunnan 666303, China ^† These authors contributed equally to this work.

* Author to whom correspondence should be addressed.

Received: 25 July 2012; in revised form: 9 October 2012 / Accepted: 29 October 2012 / Published: 16 November 2012

(This article belongs to the Special Issue Advances in Molecular Plant Biology)

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Abstract: Proteinase inhibitors play an important role in plant resistance of insects and pathogens. In this study, we characterized the serine proteinase inhibitor SaPIN2b, which is constitutively expressed in Solanum americanum trichomes and contains two conserved motifs of the proteinase inhibitor II (PIN2) family. The recombinant SaPIN2b (rSaPIN2b), which was expressed in Escherichia coli, was demonstrated to be a potent proteinase inhibitor against a panel of serine proteinases, including subtilisin A, chymotrypsin and trypsin. Moreover, rSaPIN2b also effectively inhibited the proteinase activities of midgut trypsin-like proteinases that were extracted from the devastating pest Helicoverpa armigera. Furthermore, the overexpression of SaPIN2b in transgenic tobacco plants resulted in enhanced resistance against H. armigera. Taken together, our results demonstrated that SaPIN2b is a potent serine proteinase inhibitor that may act as a protective protein in plant defense against insect attacks.

Keywords: PIN2; proteinase inhibitor; Solanum americanum; insect resistance; trichome

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