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Int. J. Mol. Sci. 2012, 13(11), 15162-15176; doi:10.3390/ijms131115162
Article

The Characterization of SaPIN2b, a Plant Trichome-Localized Proteinase Inhibitor from Solanum americanum

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Received: 25 July 2012; in revised form: 9 October 2012 / Accepted: 29 October 2012 / Published: 16 November 2012
(This article belongs to the Special Issue Advances in Molecular Plant Biology)
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Abstract: Proteinase inhibitors play an important role in plant resistance of insects and pathogens. In this study, we characterized the serine proteinase inhibitor SaPIN2b, which is constitutively expressed in Solanum americanum trichomes and contains two conserved motifs of the proteinase inhibitor II (PIN2) family. The recombinant SaPIN2b (rSaPIN2b), which was expressed in Escherichia coli, was demonstrated to be a potent proteinase inhibitor against a panel of serine proteinases, including subtilisin A, chymotrypsin and trypsin. Moreover, rSaPIN2b also effectively inhibited the proteinase activities of midgut trypsin-like proteinases that were extracted from the devastating pest Helicoverpa armigera. Furthermore, the overexpression of SaPIN2b in transgenic tobacco plants resulted in enhanced resistance against H. armigera. Taken together, our results demonstrated that SaPIN2b is a potent serine proteinase inhibitor that may act as a protective protein in plant defense against insect attacks.
Keywords: PIN2; proteinase inhibitor; Solanum americanum; insect resistance; trichome PIN2; proteinase inhibitor; Solanum americanum; insect resistance; trichome
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Luo, M.; Ding, L.-W.; Ge, Z.-J.; Wang, Z.-Y.; Hu, B.-L.; Yang, X.-B.; Sun, Q.-Y.; Xu, Z.-F. The Characterization of SaPIN2b, a Plant Trichome-Localized Proteinase Inhibitor from Solanum americanum. Int. J. Mol. Sci. 2012, 13, 15162-15176.

AMA Style

Luo M, Ding L-W, Ge Z-J, Wang Z-Y, Hu B-L, Yang X-B, Sun Q-Y, Xu Z-F. The Characterization of SaPIN2b, a Plant Trichome-Localized Proteinase Inhibitor from Solanum americanum. International Journal of Molecular Sciences. 2012; 13(11):15162-15176.

Chicago/Turabian Style

Luo, Ming; Ding, Ling-Wen; Ge, Zhi-Juan; Wang, Zhen-Yu; Hu, Bo-Lun; Yang, Xiao-Bei; Sun, Qiao-Yang; Xu, Zeng-Fu. 2012. "The Characterization of SaPIN2b, a Plant Trichome-Localized Proteinase Inhibitor from Solanum americanum." Int. J. Mol. Sci. 13, no. 11: 15162-15176.


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