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Microsatellite Mutation Rate during Allohexaploidization of Newly Resynthesized Wheat
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, the Chinese Academy of Sciences, Xining 810001, China
Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA
* Author to whom correspondence should be addressed.
Received: 30 July 2012; in revised form: 18 September 2012 / Accepted: 19 September 2012 / Published: 1 October 2012
Abstract: Simple sequence repeats (SSRs, also known as microsatellites) are known to be mutational hotspots in genomes. DNA rearrangements have also been reported to accompany allopolyploidization. A study of the effect of allopolyploidization on SSR mutation is therefore important for understanding the origin and evolutionary dynamics of SSRs in allopolyploids. Three synthesized double haploid (SynDH) populations were made from 241 interspecific F1 haploid hybrids between Triticum turgidum L. and Aegilops tauschii (Coss.) through spontaneous chromosome doubling via unreduced gametes. Mutation events were studied at 160 SSR loci in the S1 generation (the first generation after chromosome doubling) of the three SynDH populations. Of the 148260 SSR alleles investigated in S1 generation, only one mutation (changed number of repeats) was confirmed with a mutation rate of 6.74 × 10−6. This mutation most likely occurred in the respective F1 hybrid. In comparison with previously reported data, our results suggested that allohexaploidization of wheat did not increase SSR mutation rate.
Keywords: allopolyploidy; distant hybridization; microsatellite evolution; wheat
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MDPI and ACS Style
Luo, J.; Hao, M.; Zhang, L.; Chen, J.; Zhang, L.; Yuan, Z.; Yan, Z.; Zheng, Y.; Zhang, H.; Yen, Y.; Liu, D. Microsatellite Mutation Rate during Allohexaploidization of Newly Resynthesized Wheat. Int. J. Mol. Sci. 2012, 13, 12533-12543.
Luo J, Hao M, Zhang L, Chen J, Zhang L, Yuan Z, Yan Z, Zheng Y, Zhang H, Yen Y, Liu D. Microsatellite Mutation Rate during Allohexaploidization of Newly Resynthesized Wheat. International Journal of Molecular Sciences. 2012; 13(10):12533-12543.
Luo, Jiangtao; Hao, Ming; Zhang, Li; Chen, Jixiang; Zhang, Lianquan; Yuan, Zhongwei; Yan, Zehong; Zheng, Youliang; Zhang, Huaigang; Yen, Yang; Liu, Dengcai. 2012. "Microsatellite Mutation Rate during Allohexaploidization of Newly Resynthesized Wheat." Int. J. Mol. Sci. 13, no. 10: 12533-12543.