Int. J. Mol. Sci. 2012, 13(10), 12608-12628; doi:10.3390/ijms131012608
Article

Genetic Diversity and Population Structure: Implications for Conservation of Wild Soybean (Glycine soja Sieb. et Zucc) Based on Nuclear and Chloroplast Microsatellite Variation

Shuilian He 1,2 email, Yunsheng Wang 1,3 email, Sergei Volis 4 email, Dezhu Li 1,* email and Tingshuang Yi 1,* email
1 China Southwest Germplasm Bank of Wild Species, The Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China 2 University of Chinese Academy of Sciences, Beijing 100049, China 3 College of Horticulture, South China Agricultural University, Guangzhou 510642, China 4 The Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
* Authors to whom correspondence should be addressed.
Received: 27 July 2012; in revised form: 24 August 2012 / Accepted: 12 September 2012 / Published: 3 October 2012
(This article belongs to the Special Issue Advances in Molecular Plant Biology)
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Abstract: Wild soybean (Glycine soja Sieb. et Zucc) is the most important germplasm resource for soybean breeding, and is currently subject to habitat loss, fragmentation and population decline. In order to develop successful conservation strategies, a total of 604 wild soybean accessions from 43 locations sampled across its range in China, Japan and Korea were analyzed using 20 nuclear (nSSRs) and five chloroplast microsatellite markers (cpSSRs) to reveal its genetic diversity and population structure. Relatively high nSSR diversity was found in wild soybean compared with other self-pollinated species, and the region of middle and lower reaches of Yangtze River (MDRY) was revealed to have the highest genetic diversity. However, cpSSRs suggested that Korea is a center of diversity. High genetic differentiation and low gene flow among populations were detected, which is consistent with the predominant self-pollination of wild soybean. Two main clusters were revealed by MCMC structure reconstruction and phylogenetic dendrogram, one formed by a group of populations from northwestern China (NWC) and north China (NC), and the other including northeastern China (NEC), Japan, Korea, MDRY, south China (SC) and southwestern China (SWC). Contrib analyses showed that southwestern China makes the greatest contribution to the total diversity and allelic richness, and is worthy of being given conservation priority.
Keywords: Glycine soja; microsatellite; genetic diversity; genetic structure; bottleneck effect

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MDPI and ACS Style

He, S.; Wang, Y.; Volis, S.; Li, D.; Yi, T. Genetic Diversity and Population Structure: Implications for Conservation of Wild Soybean (Glycine soja Sieb. et Zucc) Based on Nuclear and Chloroplast Microsatellite Variation. Int. J. Mol. Sci. 2012, 13, 12608-12628.

AMA Style

He S, Wang Y, Volis S, Li D, Yi T. Genetic Diversity and Population Structure: Implications for Conservation of Wild Soybean (Glycine soja Sieb. et Zucc) Based on Nuclear and Chloroplast Microsatellite Variation. International Journal of Molecular Sciences. 2012; 13(10):12608-12628.

Chicago/Turabian Style

He, Shuilian; Wang, Yunsheng; Volis, Sergei; Li, Dezhu; Yi, Tingshuang. 2012. "Genetic Diversity and Population Structure: Implications for Conservation of Wild Soybean (Glycine soja Sieb. et Zucc) Based on Nuclear and Chloroplast Microsatellite Variation." Int. J. Mol. Sci. 13, no. 10: 12608-12628.

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