Next Article in Journal
Four Novel Cellulose Synthase (CESA) Genes from Birch (Betula platyphylla Suk.) Involved in Primary and Secondary Cell Wall Biosynthesis
Next Article in Special Issue
Microsatellite Mutation Rate during Allohexaploidization of Newly Resynthesized Wheat
Previous Article in Journal
Effects of Intramolecular Distance between Amyloidogenic Domains on Amyloid Aggregation
Previous Article in Special Issue
Dual Targeting and Retrograde Translocation: Regulators of Plant Nuclear Gene Expression Can Be Sequestered by Plastids
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2012, 13(10), 12182-12194; doi:10.3390/ijms131012182

SIG1, a Sigma Factor for the Chloroplast RNA Polymerase, Differently Associates with Multiple DNA Regions in the Chloroplast Chromosomes in Vivo

1
Division of Applied Biological Chemistry, Graduate School of Horticulture, Chiba University, Matsudo 648, Matsudo, Chiba 271-8510, Japan
2
Division of Bioresources, Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259-R1-29, Midori-Ku, Yokohama 226-8503, Japan
*
Author to whom correspondence should be addressed.
Received: 1 June 2012 / Revised: 5 September 2012 / Accepted: 11 September 2012 / Published: 25 September 2012
(This article belongs to the Special Issue Advances in Molecular Plant Biology)
View Full-Text   |   Download PDF [1332 KB, 19 June 2014; original version 19 June 2014]   |  

Abstract

Chloroplasts have their own DNA and gene expression systems. Transcription in chloroplasts is regulated by two types of RNA polymerase, nuclear-encoded plastid RNA polymerase (NEP) and plastid-encoded plastid RNA polymerase (PEP), and multiple sigma factors for PEP. To study transcriptional regulation in chloroplasts, a molecular genetic approach has extensively been used. However, this method may include indirect effects, and it cannot be applied to the analysis of factors essential to survival. These limitations make understanding specific regulation by transcription factors difficult. Chromatin immunoprecipitation (ChIP) is a powerful and useful tool for obtaining information on transcription-factor binding sites; it can directly detect dynamic changes in their interaction patterns in vivo. To further understand transcriptional regulation in chloroplasts, we here established a ChIP-based method in Arabidopsis thaliana and analyzed the binding pattern of a chloroplast sigma factor, SIG1. We found that SIG1 specifically binds to newly identified target promoters as well as to a set of promoters of genes whose mRNA expression is dependent on OsSIG1 in rice and that this binding changed in response to high-light stress. These results suggested that the ChIP-based approach is very useful in understanding transcriptional regulation of chloroplast genes and can overcome several problems posed by conventional methods. View Full-Text
Keywords: chloroplast; chromatin immunoprecipitation; transcriptional regulation; sigma factor; SIG1; high-light stress; Arabidopsis thaliana chloroplast; chromatin immunoprecipitation; transcriptional regulation; sigma factor; SIG1; high-light stress; Arabidopsis thaliana
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hanaoka, M.; Kato, M.; Anma, M.; Tanaka, K. SIG1, a Sigma Factor for the Chloroplast RNA Polymerase, Differently Associates with Multiple DNA Regions in the Chloroplast Chromosomes in Vivo. Int. J. Mol. Sci. 2012, 13, 12182-12194.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top