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Isolation and Characterization of Sixteen Polymorphic Microsatellite Loci in the Golden Apple Snail Pomacea canaliculata
Nanjing Institute of Environmental Sciences under Ministry of Environmental Protection, 8 Jiangwangmiao Street, Nanjing 210042, Jiangsu, China
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, Jiangsu, China
* Author to whom correspondence should be addressed.
Received: 4 July 2011; in revised form: 4 September 2011 / Accepted: 9 September 2011 / Published: 16 September 2011
Abstract: We report the characterization of 16 polymorphic microsatellite markers in the golden apple snail, Pomacea canaliculata, a pest registered in the list of “100 of the world’s worst invasive alien species”. The fast isolation by AFLP (Amplified Fragment Length Polymorphism) of sequences containing repeats (FIASCO) method was used to isolate microsatellite loci, and polymorphism was explored with 29 individuals collected in an invasive region from China. These primers showed a number of alleles per locus ranging from three to 13. The ranges of observed and expected heterozygosity were 0.310–0.966 and 0.523–0.898, respectively. These microsatellite markers described here will be useful for population genetic studies of P. canaliculata.
Keywords: golden apple snail; invasive species; microsatellite loci; Pomacea canaliculata
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MDPI and ACS Style
Chen, L.; Xu, H.; Li, H.; Wu, J.; Ding, H.; Liu, Y. Isolation and Characterization of Sixteen Polymorphic Microsatellite Loci in the Golden Apple Snail Pomacea canaliculata. Int. J. Mol. Sci. 2011, 12, 5993-5998.
Chen L, Xu H, Li H, Wu J, Ding H, Liu Y. Isolation and Characterization of Sixteen Polymorphic Microsatellite Loci in the Golden Apple Snail Pomacea canaliculata. International Journal of Molecular Sciences. 2011; 12(9):5993-5998.
Chen, Lian; Xu, Haigen; Li, Hong; Wu, Jun; Ding, Hui; Liu, Yan. 2011. "Isolation and Characterization of Sixteen Polymorphic Microsatellite Loci in the Golden Apple Snail Pomacea canaliculata." Int. J. Mol. Sci. 12, no. 9: 5993-5998.