Caffeine Abolishes the Ultraviolet-Induced REV3 Translesion Replication Pathway in Mouse Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Detection of UV-Induced Translesion Replication in Mouse Cells
2.2. UV-TLS Was Mostly Abolished in Rev3 Knockout MEFs
2.3. Rev3 Transgene Restored UV-TLS in Rev3 Knockout Mouse Cells
2.4. Introduction of the Rev3 Expression Vector Restored UV-TLS in Rev3 Knockout MEFs
2.5. Correlation of UV Survival with UV-TLS
2.6. Where Is the Target for UV-TLS Inhibition by Caffeine or Proteasome Inhibitors?
3. Experimental Section
3.1. Mouse Cell Culture
3.2. UV Irradiation and Translesion Replication
3.3. Alkaline Sucrose Density Gradient Centrifugation
3.4. Construction of the Mouse Rev3-Expressing Plasmid
3.5. Transfection with the Plasmid and Selection of Stable Transformant Strains
3.6. Survival Assay of UV-Irradiated Cells
4. Conclusions
Acknowledgments
References and Notes
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Takezawa, J.; Aiba, N.; Kajiwara, K.; Yamada, K. Caffeine Abolishes the Ultraviolet-Induced REV3 Translesion Replication Pathway in Mouse Cells. Int. J. Mol. Sci. 2011, 12, 8513-8529. https://doi.org/10.3390/ijms12128513
Takezawa J, Aiba N, Kajiwara K, Yamada K. Caffeine Abolishes the Ultraviolet-Induced REV3 Translesion Replication Pathway in Mouse Cells. International Journal of Molecular Sciences. 2011; 12(12):8513-8529. https://doi.org/10.3390/ijms12128513
Chicago/Turabian StyleTakezawa, Jun, Naomi Aiba, Kagemasa Kajiwara, and Kouichi Yamada. 2011. "Caffeine Abolishes the Ultraviolet-Induced REV3 Translesion Replication Pathway in Mouse Cells" International Journal of Molecular Sciences 12, no. 12: 8513-8529. https://doi.org/10.3390/ijms12128513