Effects of Soybean Agglutinin on Intestinal Barrier Permeability and Tight Junction Protein Expression in Weaned Piglets
Abstract
:1. Introduction
2. Results and Discussion
2.1. Purification of Glycinin and β-Conglycinin
2.2. Experimental Design
2.3. Determination of d-Lactic Acid in Plasma
2.4. Determination of Diamine Oxidase (DAO) in Ileal Mucosa
2.5. Immunohistochemistry
2.6. Immunoblotting
2.7. Statistical Analysis
3. Results
3.1. d-Lactic Acid in Plasma and DAO in Ileal Mucosa
3.2. Occludin or ZO-1 Expression in Mid-Jejunum Tissue
4. Discussions
5. Conclusions
Acknowledgments
References
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Ingredients (%) | Nutrient contents 3 (%) | ||
---|---|---|---|
Maize | 22.00 | Crude protein | 23.92 |
Fish meal | 11.20 | Digestive energy (MJ/kg) | 16.91 |
Whey powder | 18.00 | Calcium | 0.86 |
Milk powder | 48.00 | Phosphorus | 0.83 |
Salt | 0.20 | Lysine | 2.00 |
Vitamin premix 1 | 0.10 | ||
Vitamin premix 2 | 0.20 | ||
Limestone | 0.30 |
Groups | 0% SBA | 0.05% SBA | 0.1% SBA | 0.15% SBA | 0.2 % SBA |
---|---|---|---|---|---|
DAO/(U·g−1) | 11.69 ± 2.67 a | 10.28 ± 1.54 a,b | 7.97 ± 1.27 bc | 7.57 ± 1.56 c | 6.15 ± 1.71 c |
d-lactic acid/(μg·mL−1) | 2.11 ± 0.26 a | 2.09 ± 0.29 a | 2.29 ± 0.12 ab | 2.45 ± 0.09 b,c | 2.62 ± 0.18 c |
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Zhao, Y.; Qin, G.; Sun, Z.; Che, D.; Bao, N.; Zhang, X. Effects of Soybean Agglutinin on Intestinal Barrier Permeability and Tight Junction Protein Expression in Weaned Piglets. Int. J. Mol. Sci. 2011, 12, 8502-8512. https://doi.org/10.3390/ijms12128502
Zhao Y, Qin G, Sun Z, Che D, Bao N, Zhang X. Effects of Soybean Agglutinin on Intestinal Barrier Permeability and Tight Junction Protein Expression in Weaned Piglets. International Journal of Molecular Sciences. 2011; 12(12):8502-8512. https://doi.org/10.3390/ijms12128502
Chicago/Turabian StyleZhao, Yuan, Guixin Qin, Zewei Sun, Dongsheng Che, Nan Bao, and Xiaodong Zhang. 2011. "Effects of Soybean Agglutinin on Intestinal Barrier Permeability and Tight Junction Protein Expression in Weaned Piglets" International Journal of Molecular Sciences 12, no. 12: 8502-8512. https://doi.org/10.3390/ijms12128502