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Int. J. Mol. Sci. 2011, 12(11), 8302-8315; doi:10.3390/ijms12118302
Article

Coenzyme Q10 Ameliorates Ultraviolet B Irradiation Induced Cell Death Through Inhibition of Mitochondrial Intrinsic Cell Death Pathway

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Received: 23 August 2011; in revised form: 8 October 2011 / Accepted: 21 November 2011 / Published: 24 November 2011
(This article belongs to the Special Issue UV-Induced Cell Death)
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Abstract: Ultraviolet B (UVB) induces cell death by increasing free radical production, activating apoptotic cell death pathways and depolarizing mitochondrial membrane potential. Coenzyme Q10 (CoQ10), an essential cofactor in the mitochondrial electron transport chain, serves as a potent antioxidant in the mitochondria. The aim of the present study is to establish whether CoQ10 is capable of protecting neuronal cells against UVB-induced damage. Murine hippocampal HT22 cells were treated with 0.01, 0.1 or 1 µM of CoQ10 3 or 24 h prior to the cells being exposed to UVB irradiation. The CoQ10 concentrations were maintained during irradiation and 24 h post-UVB. Cell viability was assessed by counting viable cells and MTT conversion assay. Superoxide production and mitochondrial membrane potential were measured using fluorescent probes. Levels of cleaved caspase-9, caspase-3, and apoptosis-inducing factor (AIF) were detected using immunocytochemistry and Western blotting. The results showed that UVB irradiation decreased cell viability and such damaging effect was associated with increased superoxide production, mitochondrial depolarization, and activation of caspase-9 and caspase-3. Treatment with CoQ10 at three different concentrations started 24 h before UVB exposure significantly increased the cell viability. The protective effect of CoQ10 was associated with reduction in superoxide production, normalization of mitochondrial membrane potential and inhibition of caspase-9 and caspase-3 activation. It is concluded that the neuroprotective effect of CoQ10 results from inhibiting oxidative stress and blocking caspase-3 dependent cell death pathway.
Keywords: apoptosis; caspase; cell death pathway; coenzyme Q10; mitochondria; ubiquinone 10; ultraviolet apoptosis; caspase; cell death pathway; coenzyme Q10; mitochondria; ubiquinone 10; ultraviolet
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Jing, L.; Kumari, S.; Mendelev, N.; Li, P.A. Coenzyme Q10 Ameliorates Ultraviolet B Irradiation Induced Cell Death Through Inhibition of Mitochondrial Intrinsic Cell Death Pathway. Int. J. Mol. Sci. 2011, 12, 8302-8315.

AMA Style

Jing L, Kumari S, Mendelev N, Li PA. Coenzyme Q10 Ameliorates Ultraviolet B Irradiation Induced Cell Death Through Inhibition of Mitochondrial Intrinsic Cell Death Pathway. International Journal of Molecular Sciences. 2011; 12(11):8302-8315.

Chicago/Turabian Style

Jing, Li; Kumari, Santosh; Mendelev, Natalia; Li, P. Andy. 2011. "Coenzyme Q10 Ameliorates Ultraviolet B Irradiation Induced Cell Death Through Inhibition of Mitochondrial Intrinsic Cell Death Pathway." Int. J. Mol. Sci. 12, no. 11: 8302-8315.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert