Int. J. Mol. Sci. 2011, 12(11), 7635-7651; doi:10.3390/ijms12117635
Article

A 1HNMR-Based Metabonomics Study of Postmenopausal Osteoporosis and Intervention Effects of Er-Xian Decoction in Ovariectomized Rats

1email, 2email, 1,3email, 1,3email, 1email, 1,* email and 1,* email
Received: 30 July 2011; in revised form: 29 October 2011 / Accepted: 31 October 2011 / Published: 8 November 2011
(This article belongs to the Section Molecular Diagnostics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A metabonomics method using 1H nuclear magnetic resonance spectroscopy (1HNMR) was applied to obtain a systematic view of the development and progression of postmenopausal osteoporosis. Using partial least squares discriminant analysis (PLS-DA), 26 and 34 characteristic resonances were found respectively in urine and plasma of ovariectomized rats (Variable importance, VIP value ≥1.0), and the significant altered metabolites identified in the plasma and urine were 10 and 9, respectively. Changes in these metabolites were related to the pathways of lipid, energy and amino acid metabolism, some of which involved the oxidative system. The described method was also used to analyze the therapeutic effects of Er-Xian Decoction (EXD), a traditional Chinese medicine widely used in the clinical treatment of osteoporosis in China. The results showed that EXD administration could provide satisfactory effects on osteoporosis through partially regulating the perturbed pathways of lipid, energy and amino acid metabolism and improving the anti-oxidative ability.
Keywords: 1HNMR; metabonomics; ovariectomized rats; postmenopausal osteoporosis; Er-Xian Decoction
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MDPI and ACS Style

Xue, L.; Wang, Y.; Liu, L.; Zhao, L.; Han, T.; Zhang, Q.; Qin, L. A 1HNMR-Based Metabonomics Study of Postmenopausal Osteoporosis and Intervention Effects of Er-Xian Decoction in Ovariectomized Rats. Int. J. Mol. Sci. 2011, 12, 7635-7651.

AMA Style

Xue L, Wang Y, Liu L, Zhao L, Han T, Zhang Q, Qin L. A 1HNMR-Based Metabonomics Study of Postmenopausal Osteoporosis and Intervention Effects of Er-Xian Decoction in Ovariectomized Rats. International Journal of Molecular Sciences. 2011; 12(11):7635-7651.

Chicago/Turabian Style

Xue, Liming; Wang, Yin; Liu, Lei; Zhao, Lu; Han, Ting; Zhang, Qiaoyan; Qin, Luping. 2011. "A 1HNMR-Based Metabonomics Study of Postmenopausal Osteoporosis and Intervention Effects of Er-Xian Decoction in Ovariectomized Rats." Int. J. Mol. Sci. 12, no. 11: 7635-7651.

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