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Molecular Systematics of Polygonum minus Huds. Based on ITS Sequences
Institute of Systems Biology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia
School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
* Author to whom correspondence should be addressed.
Received: 19 August 2011; in revised form: 8 October 2011 / Accepted: 18 October 2011 / Published: 7 November 2011
Abstract: Plastid trnL-trnF and nuclear ribosomal ITS sequences were obtained from selected wild-type individuals of Polygonum minus Huds. in Peninsular Malaysia. The 380 bp trnL-trnF sequences of the Polygonum minus accessions were identical. Therefore, the trnL-trnF failed to distinguish between the Polygonum minus accessions. However, the divergence of ITS sequences (650 bp) among the Polygonum minus accessions was 1%, indicating that these accessions could be distinguished by the ITS sequences. A phylogenetic relationship based on the ITS sequences was inferred using neighbor-joining, maximum parsimony and Bayesian inference. All of the tree topologies indicated that Polygonum minus from Peninsular Malaysia is unique and different from the synonymous Persicaria minor (Huds.) Opiz and Polygonum kawagoeanum Makino.
Keywords: ITS; phylogenetics; plastid DNA; Polygonum minus; trnL-trnF
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Bunawan, H.; Choong, C.Y.; Md-Zain, B.M.; Baharum, S.N.; Noor, N.M. Molecular Systematics of Polygonum minus Huds. Based on ITS Sequences. Int. J. Mol. Sci. 2011, 12, 7626-7634.
Bunawan H, Choong CY, Md-Zain BM, Baharum SN, Noor NM. Molecular Systematics of Polygonum minus Huds. Based on ITS Sequences. International Journal of Molecular Sciences. 2011; 12(11):7626-7634.
Bunawan, Hamidun; Choong, Chee Yen; Md-Zain, Badrul Munir; Baharum, Syarul Nataqain; Noor, Normah Mohd. 2011. "Molecular Systematics of Polygonum minus Huds. Based on ITS Sequences." Int. J. Mol. Sci. 12, no. 11: 7626-7634.