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Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo
Department of Gynecology, Nanjing Medical University of Hangzhou Hospital, 261 Huansha Road, Hangzhou, Zhejiang 310006, China
* Author to whom correspondence should be addressed.
Received: 30 November 2010; in revised form: 28 December 2010 / Accepted: 10 January 2011 / Published: 18 January 2011
Abstract: The present study was designed to investigate the combined effects of cyclooxygenase (COX)-1 and COX-2 selective inhibitors on human ovarian SKOV-3 carcinoma cells xenograft-bearing mice. The animals were treated with 3 mg/kg SC-560 (a COX-1 selective inhibitor) alone, 25 mg/kg celecoxib (a COX-2 selective inhibitor) alone, or SC-560/celecoxib by gavage, twice a day for three weeks. To test the mechanism of inhibition of tumor growth by COX selective inhibitors, the index of proliferating cells in tumor tissues was determined by immunostaining and the index of apoptotic cells by the terminal-deoxynucleotidyl-transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) method. The inhibitory rate on tumor growth in the combination group was 35.54% which is significant statistically compared with that of the control group (P < 0.05). In the combination group, the index of cell proliferation and apoptosis were 12.40% and 51.03% respectively, which are significant statistically compared with those of the control group (22.56%, 19.07%, all P < 0.05). These studies indicate that synergism between two COX inhibitors and inhibitor combination treatment has particular potential for chemoprevention of ovarian cancer growth.
Keywords: ovarian carcinoma; mice; cyclooxygenase selective inhibitor; cell proliferation; apoptosis
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MDPI and ACS Style
Li, W.; Wang, J.; Jiang, H.-R.; Xu, X.-L.; Zhang, J.; Liu, M.-L.; Zhai, L.-Y. Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo. Int. J. Mol. Sci. 2011, 12, 668-681.
Li W, Wang J, Jiang H-R, Xu X-L, Zhang J, Liu M-L, Zhai L-Y. Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo. International Journal of Molecular Sciences. 2011; 12(1):668-681.
Li, Wei; Wang, Jie; Jiang, Hong-Ru; Xu, Xiao-Li; Zhang, Jun; Liu, Mei-Lin; Zhai, Ling-Yun. 2011. "Combined Effects of Cyclooxygenase-1 and Cyclooxygenase-2 Selective Inhibitors on Ovarian Carcinoma in Vivo." Int. J. Mol. Sci. 12, no. 1: 668-681.