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• Gustafsson, J
• Oehler, M
• Ruszkiewicz, A
• McColl, S
• Hoffmann, P
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• Gustafsson, J
• Oehler, M
• Ruszkiewicz, A
• McColl, S
• Hoffmann, P
• [+] on PubMed
• Gustafsson, J
• Oehler, M
• Ruszkiewicz, A
• McColl, S
• Hoffmann, P

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Int. J. Mol. Sci. 2011, 12(1), 773-794; doi:10.3390/ijms12010773

Review

MALDI Imaging Mass Spectrometry (MALDI-IMS)―Application of Spatial Proteomics for Ovarian Cancer Classification and Diagnosis

Johan O. R. Gustafsson ¹ , Martin K. Oehler ² , Andrew Ruszkiewicz ³ , Shaun R. McColl ¹  and Peter Hoffmann ^1,*

¹ Adelaide Proteomics Centre, School of Molecular and Biomedical Science, The University of Adelaide, SA 5005, Adelaide, Australia ² Robinson Institute, Research Centre for Reproductive Health, School of Paediatrics and Reproductive Health, The University of Adelaide, SA 5005, Adelaide, Australia ³ Surgical Pathology, SA Pathology, SA 5000, Adelaide, Australia

* Author to whom correspondence should be addressed.

Received: 1 December 2010; in revised form: 10 January 2011 / Accepted: 17 January 2011 / Published: 21 January 2011

(This article belongs to the Special Issue Cancer Molecules in Ovarian Cancer)

Download PDF Full-Text [616 KB, uploaded 21 January 2011 16:39 CET]

Abstract: MALDI imaging mass spectrometry (MALDI-IMS) allows acquisition of mass data for metabolites, lipids, peptides and proteins directly from tissue sections. IMS is typically performed either as a multiple spot profiling experiment to generate tissue specific mass profiles, or a high resolution imaging experiment where relative spatial abundance for potentially hundreds of analytes across virtually any tissue section can be measured. Crucially, imaging can be achieved without prior knowledge of tissue composition and without the use of antibodies. In effect MALDI-IMS allows generation of molecular data which complement and expand upon the information provided by histology including immuno-histochemistry, making its application valuable to both cancer biomarker research and diagnostics. The current state of MALDI-IMS, key biological applications to ovarian cancer research and practical considerations for analysis of peptides and proteins on ovarian tissue are presented in this review.

Keywords: MALDI; imaging; mass spectrometry; ovarian cancer; grading; biomarker

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