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Int. J. Mol. Sci. 2011, 12(1), 570-587; doi:10.3390/ijms12010570
Article

Immunomodulatory Activity of Dietary Fiber: Arabinoxylan and Mixed-Linked Beta-Glucan Isolated from Barley Show Modest Activities in Vitro

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Received: 26 November 2010 / Revised: 20 December 2010 / Accepted: 4 January 2011 / Published: 18 January 2011
(This article belongs to the Special Issue Dietary Fibre: Biochemistry and Nutritional Science)
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Abstract

High intake of dietary fiber is claimed to protect against development of colorectal cancer. Barley is a rich source of dietary fiber, and possible immunomodulatory effects of barley polysaccharides might explain a potential protective effect. Dietary fiber was isolated by extraction and enzyme treatment. A mixed-linked β-glucan (WSM-TPX, 96.5% β-glucan, Mw 886 kDa), an arabinoxylan (WUM-BS-LA, 96.4% arabinoxylan, Mw 156 kDa), a mixed-linked β-glucan rich fraction containing 10% arabinoxylan (WSM-TP) and an arabinoxylan rich fraction containing 30% mixed-linked β-glucan (WUM-BS) showed no significant effect on IL-8 secretion and proliferation of two intestinal epithelial cell lines, Caco-2 and HT-29, and had no significant effect on the NF-κB activity in the monocytic cell line U937-3κB-LUC. Further enriched arabinoxylan fractions (WUM-BS-LA) from different barley varieties (Tyra, NK96300, SB94897 and CDCGainer) were less active than the mixed-linked β-glucan rich fractions (WSM-TP and WSM-TPX) in the complement-fixing test. The mixed-linked β-glucan rich fraction from NK96300 and CDCGainer showed similar activities as the positive control while mixed‑linked β-glucan rich fractions from Tyra and SB94897 were less active. From these results it is concluded that the isolated high molecular weight mixed-linked β-glucans and arabinoxylans from barley show low immunological responses in selected in vitro test systems and thus possible anti-colon cancer effects of barley dietary fiber cannot be explained by our observations.
Keywords: arabinoxylan; mixed-linked β-glucan; barley; Caco-2; complement-fixing test; dietary fiber; HT-29; IL-8; U937; NF-kappaB arabinoxylan; mixed-linked β-glucan; barley; Caco-2; complement-fixing test; dietary fiber; HT-29; IL-8; U937; NF-kappaB
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Samuelsen, A.B.; Rieder, A.; Grimmer, S.; Michaelsen, T.E.; Knutsen, S.H. Immunomodulatory Activity of Dietary Fiber: Arabinoxylan and Mixed-Linked Beta-Glucan Isolated from Barley Show Modest Activities in Vitro. Int. J. Mol. Sci. 2011, 12, 570-587.

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