Screening and Initial Binding Assessment of Fumonisin B1 Aptamers
AbstractFumonisins are mycotoxins produced by Fusarium verticillioides and F. proliferatum, fungi that are ubiquitous in corn (maize). Insect damage and some other environmental conditions result in the accumulation of fumonisins in corn-based products worldwide. Current methods of fumonisin detection rely on the use of immunoaffinity columns and high-performance liquid chromatography (HPLC). The use of aptamers offers a good alternative to the use of antibodies in fumonisin cleanup and detection due to lower costs and improved stability. Aptamers are single-stranded oligonucleotides that are selected using Systematic Evolution of Ligands by EXponential enrichment (SELEX) for their ability to bind to targets with high affinity and specificity. Sequences obtained after 18 rounds of SELEX were screened for their ability to bind to fumonisin B1. Six unique sequences were obtained, each showing improved binding to fumonisin B1 compared to controls. Sequence FB1 39 binds to fumonisin with a dissociation constant of 100 ± 30 nM and shows potential for use in fumonisin biosensors and solid phase extraction columns.
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McKeague, M.; Bradley, C.R.; Girolamo, A.D.; Visconti, A.; Miller, J.D.; DeRosa, M.C. Screening and Initial Binding Assessment of Fumonisin B1 Aptamers. Int. J. Mol. Sci. 2010, 11, 4864-4881.
McKeague M, Bradley CR, Girolamo AD, Visconti A, Miller JD, DeRosa MC. Screening and Initial Binding Assessment of Fumonisin B1 Aptamers. International Journal of Molecular Sciences. 2010; 11(12):4864-4881.Chicago/Turabian Style
McKeague, Maureen; Bradley, Charlotte R.; Girolamo, Annalisa De; Visconti, Angelo; Miller, J. David; DeRosa, Maria C. 2010. "Screening and Initial Binding Assessment of Fumonisin B1 Aptamers." Int. J. Mol. Sci. 11, no. 12: 4864-4881.