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Screening and Initial Binding Assessment of Fumonisin B1 Aptamers
Chemistry Department, Carleton University, 1125 Colonel By Drive, Ottawa, ON, Canada K1S 5B6
Institute of Sciences of Food Production, National Research Council, via Amendola 122/O, 10 70126 Bari, Italy
* Author to whom correspondence should be addressed.
Received: 1 November 2010; in revised form: 18 November 2010 / Accepted: 18 November 2010 / Published: 26 November 2010
Abstract: Fumonisins are mycotoxins produced by Fusarium verticillioides and F. proliferatum, fungi that are ubiquitous in corn (maize). Insect damage and some other environmental conditions result in the accumulation of fumonisins in corn-based products worldwide. Current methods of fumonisin detection rely on the use of immunoaffinity columns and high-performance liquid chromatography (HPLC). The use of aptamers offers a good alternative to the use of antibodies in fumonisin cleanup and detection due to lower costs and improved stability. Aptamers are single-stranded oligonucleotides that are selected using Systematic Evolution of Ligands by EXponential enrichment (SELEX) for their ability to bind to targets with high affinity and specificity. Sequences obtained after 18 rounds of SELEX were screened for their ability to bind to fumonisin B1. Six unique sequences were obtained, each showing improved binding to fumonisin B1 compared to controls. Sequence FB1 39 binds to fumonisin with a dissociation constant of 100 ± 30 nM and shows potential for use in fumonisin biosensors and solid phase extraction columns.
Keywords: aptamer; fumonisin B1; mycotoxins; DNA; SELEX; toxins; maize; corn; binding affinity; molecular recognition
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McKeague, M.; Bradley, C.R.; Girolamo, A.D.; Visconti, A.; Miller, J.D.; DeRosa, M.C. Screening and Initial Binding Assessment of Fumonisin B1 Aptamers. Int. J. Mol. Sci. 2010, 11, 4864-4881.
McKeague M, Bradley CR, Girolamo AD, Visconti A, Miller JD, DeRosa MC. Screening and Initial Binding Assessment of Fumonisin B1 Aptamers. International Journal of Molecular Sciences. 2010; 11(12):4864-4881.
McKeague, Maureen; Bradley, Charlotte R.; Girolamo, Annalisa De; Visconti, Angelo; Miller, J. David; DeRosa, Maria C. 2010. "Screening and Initial Binding Assessment of Fumonisin B1 Aptamers." Int. J. Mol. Sci. 11, no. 12: 4864-4881.