Int. J. Mol. Sci. 2010, 11(12), 4882-4890; doi:10.3390/ijms11124882

In Situ and in Vivo Study of Nasal Absorption of Paeonol in Rats

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Received: 12 October 2010; in revised form: 10 November 2010 / Accepted: 10 November 2010 / Published: 26 November 2010
(This article belongs to the Section Bioactives and Nutraceuticals)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The objective of this work was to study the in situ and in vivo nasal absorption of paeonol. A novel single pass in situ nasal perfusion technique was applied to examine the rate and extent of nasal absorption of paeonol by rats. Various experimental conditions, such as perfusion rate, pH, osmotic pressure and drug concentration, were investigated. The in situ experiments showed that the nasal absorption of paeonol was not dependent on drug concentration, and fitted a first order process. The absorption rate constant, Ka, increased with an increase in perfusion speed. Paeonol was better absorbed in acidic solutions than in neutral or alkaline solutions. The value of Ka was higher in a hypertonic environment than under isotonic or hypotonic conditions. In vivo studies of paeonol absorption were carried out in rats and the pharmacokinetics parameters of intranasal (i.n.) and intragastric (i.g.) administration were compared with intravenous (i.v.) administration. The bioavailabilities of paeonol were 52.37% and 15.81% for i.n. and i.g, respectively, while Tmax values were 3.05 ± 1.46 min and 6.30 ± 0.70 min. MRT (Mean Residence Time) were 23.19 ± 6.46 min, 41.49 ± 2.96 min and 23.09 ± 5.88 min for i.n., i.g. and i.v. methods, respectively. The results demonstrate that paeonol could be absorbed promptly and thoroughly by i.n. administration in rats.
Keywords: paeonol; nasal absorption; in situ; single pass perfusion technique; pharmacokinetics
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MDPI and ACS Style

Chen, X.; Lu, Y.; Du, S.; Xu, B.; Wang, S.; Zhai, Y.; Song, X.; Li, P. In Situ and in Vivo Study of Nasal Absorption of Paeonol in Rats. Int. J. Mol. Sci. 2010, 11, 4882-4890.

AMA Style

Chen X, Lu Y, Du S, Xu B, Wang S, Zhai Y, Song X, Li P. In Situ and in Vivo Study of Nasal Absorption of Paeonol in Rats. International Journal of Molecular Sciences. 2010; 11(12):4882-4890.

Chicago/Turabian Style

Chen, Xiaolan; Lu, Yang; Du, Shouying; Xu, Bing; Wang, Shan; Zhai, Yongsong; Song, Xiao; Li, Pengyue. 2010. "In Situ and in Vivo Study of Nasal Absorption of Paeonol in Rats." Int. J. Mol. Sci. 11, no. 12: 4882-4890.

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