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Int. J. Mol. Sci. 2010, 11(12), 4826-4842; doi:10.3390/ijms11124826
Article

An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics

1, 1, 1, 1, 2, 3, 3, 4, 4, 5, 5, 6 and 1,*
Received: 11 October 2010; in revised form: 10 November 2010 / Accepted: 24 November 2010 / Published: 26 November 2010
(This article belongs to the Special Issue Advances in Molecular Diagnostics)
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Abstract: The drugs based on platinum metals represent one of the oldest, but also one of the most effective groups of chemotherapeutic agents. Thanks to many clinical studies it is known that resistance of tumor cells to drugs is a frequent cause of chemotherapy failure. With regard to platinum based drugs, multidrug resistance can also be connected with increased expression of low-molecular weight protein metallothionein (MT). This study aimed at investigating the interactions of MT with cisplatin or carboplatin, using the adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction (AdTS DPV Brdicka reaction), and a comparison of in vitro results with results obtained in vivo. The results obtained from the in vitro study show a strong affinity between platinum based drugs and MT. Further, we analyzed extracts of neuroblastoma cell lines treated with cisplatin or carboplatin. It is clear that neuroblastoma UKF-NB-4 cisplatin-resistant and cisplatin-sensitive cell lines unlikely respond to the presence of the platinum-based cytostatics cisplatin and carboplatin. Finally, we determined the level of MT in samples from rabbits treated with carboplatin and patients with retinoblastoma treated with the same drug.
Keywords: tumor disease; metallothionein; platinum based anticancer drugs; anticancer therapy; resistance; retinoblastoma tumor disease; metallothionein; platinum based anticancer drugs; anticancer therapy; resistance; retinoblastoma
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Krizkova, S.; Fabrik, I.; Huska, D.; Adam, V.; Babula, P.; Hrabeta, J.; Eckschlager, T.; Pochop, P.; Darsova, D.; Kukacka, J.; Prusa, R.; Trnkova, L.; Kizek, R. An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics. Int. J. Mol. Sci. 2010, 11, 4826-4842.

AMA Style

Krizkova S, Fabrik I, Huska D, Adam V, Babula P, Hrabeta J, Eckschlager T, Pochop P, Darsova D, Kukacka J, Prusa R, Trnkova L, Kizek R. An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics. International Journal of Molecular Sciences. 2010; 11(12):4826-4842.

Chicago/Turabian Style

Krizkova, Sona; Fabrik, Ivo; Huska, Dalibor; Adam, Vojtech; Babula, Petr; Hrabeta, Jan; Eckschlager, Tomas; Pochop, Pavel; Darsova, Denisa; Kukacka, Jiri; Prusa, Richard; Trnkova, Libuse; Kizek, Rene. 2010. "An Adsorptive Transfer Technique Coupled with Brdicka Reaction to Reveal the Importance of Metallothionein in Chemotherapy with Platinum Based Cytostatics." Int. J. Mol. Sci. 11, no. 12: 4826-4842.



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