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Effect of Oxidative Damage Due to Excessive Protein Ingestion on Pancreas Function in Mice
Institute of Food Science and Engineering, Jilin Agricultural University, Jilin, Changchun 130118, China
Jiutai City Traditional Chinese Medical Hospital, Jiutai, Jilin, Changchun 130500, China
* Author to whom correspondence should be addressed.
Received: 31 October 2010; in revised form: 9 November 2010 / Accepted: 10 November 2010 / Published: 16 November 2010
Abstract: The present study was undertaken to evaluate the effect of oxidative damage due to excessive protein diet on pancreas function in mice. For this purpose, thirty male (C57BL/6J) mice were randomly divided into three groups and fed on different diets as follows: group 1 was fed on a normal diet, group 2 was fed on an excessive protein diet and group 3 was fed on an excessive protein diet supplemented with 0.06 g/kg cysteamine. Each group was fed for 2 weeks, and then pancreas samples were collected to examine oxidative and antioxidant parameters and pancreas function. The results showed that ingestion of an excessive protein diet markedly increased contents of malondialdehyde (MDA) and decreased T-AOC and activities of antioxidants SOD and GSH-Px, compared with a normal diet (P < 0.05). Pancreas weight and concentration of protein, DNA and RNA were significantly higher (P < 0.05), digestive enzyme activities were significantly lower and levels of somatostatin and insulin were higher in mice fed with an excessive protein diet than those fed with a normal protein diet. In the group fed with excessive protein diet supplemented with cysteamine, oxidative stress was mitigated and pancreas function was improved. These data demonstrate that excessive protein ingestion could increase oxidative damage of free radicals on pancreas function through destroying the balance of oxidants and antioxidants.
Keywords: excessive protein diet; pancreas; oxidative damage; antioxidant; mice
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MDPI and ACS Style
Gu, C.; Xu, H. Effect of Oxidative Damage Due to Excessive Protein Ingestion on Pancreas Function in Mice. Int. J. Mol. Sci. 2010, 11, 4591-4600.
Gu C, Xu H. Effect of Oxidative Damage Due to Excessive Protein Ingestion on Pancreas Function in Mice. International Journal of Molecular Sciences. 2010; 11(11):4591-4600.
Gu, Chunmei; Xu, Huiyong. 2010. "Effect of Oxidative Damage Due to Excessive Protein Ingestion on Pancreas Function in Mice." Int. J. Mol. Sci. 11, no. 11: 4591-4600.