β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation
AbstractIn the present study we evaluated the anti-angiogenic activities of β-escin (the major active compound of Aesculus hippocastanum L. seeds). Human umbilical-vein endothelial cells (HUVECs) were used as an in vitro model for studying the molecular mechanism underlying the anti-angiogenic effect of β-escin. We investigated the in vitro effects on proliferation, migration, and tube formation of HUVECs and in vivo anti-angiogenic activity was evaluated in a chick chorioallantoic membrane (CAM) angiogenesis assay. Moreover, the effect on gene expressions was determined by the RT2 ProfilerTM human angiogenesis PCR Array. It was found that β-escin exerts inhibitory effect on the basic fibroblast growth factor (bFGF)-induced proliferation, migration and tube formation, as well as CAM angiogenesis in vivo. The inhibition of critical steps of angiogenic process observed with β-escin could be partially explained by suppression of Akt activation in response to bFGF. Moreover, the anti-angiogenic effects of β-escin could also be mediated via inhibition of EFNB2 and FGF-1 gene expressions in endothelial cells. In conclusion, β-escin affects endothelial cells as a negative mediator of angiogenesis in vitro and in vivo and may therefore be considered as a promising candidate for further research elucidating its underlying mechanism of action. View Full-Text
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Varinská, L.; Fáber, L.; Kello, M.; Petrovová, E.; Balážová, Ľ.; Solár, P.; Čoma, M.; Urdzík, P.; Mojžiš, J.; Švajdlenka, E.; Mučaji, P.; Gál, P. β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation. Molecules 2018, 23, 197.
Varinská L, Fáber L, Kello M, Petrovová E, Balážová Ľ, Solár P, Čoma M, Urdzík P, Mojžiš J, Švajdlenka E, Mučaji P, Gál P. β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation. Molecules. 2018; 23(1):197.Chicago/Turabian Style
Varinská, Lenka; Fáber, Lenka; Kello, Martin; Petrovová, Eva; Balážová, Ľudmila; Solár, Peter; Čoma, Matúš; Urdzík, Peter; Mojžiš, Ján; Švajdlenka, Emil; Mučaji, Pavel; Gál, Peter. 2018. "β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation." Molecules 23, no. 1: 197.
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