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Molecules 2017, 22(7), 1222; doi:10.3390/molecules22071222

Application of Ammonium Persulfate for Selective Oxidation of Guanines for Nucleic Acid Sequencing

1,2,†
,
1,2,†
,
1,2
,
1,2
,
1,2
,
1,2
,
3
and
1,2,*
1
Key Laboratory of Biomedical Polymers of Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei, China
2
The Institute for Advanced Studies, Wuhan University, Wuhan 430072, Hubei, China
3
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan 430062, Hubei, China
These authors contributed equally to this work and should be considered as co-first authors.
*
Author to whom correspondence should be addressed.
Received: 12 June 2017 / Revised: 14 July 2017 / Accepted: 17 July 2017 / Published: 21 July 2017
View Full-Text   |   Download PDF [2308 KB, uploaded 21 July 2017]   |  

Abstract

Nucleic acids can be sequenced by a chemical procedure that partially damages the nucleotide positions at their base repetition. Many methods have been reported for the selective recognition of guanine. The accurate identification of guanine in both single and double regions of DNA and RNA remains a challenging task. Herein, we present a new, non-toxic and simple method for the selective recognition of guanine in both DNA and RNA sequences via ammonium persulfate modification. This strategy can be further successfully applied to the detection of 5-methylcytosine by using PCR. View Full-Text
Keywords: guanine; ammonium persulfate; nucleic acid guanine; ammonium persulfate; nucleic acid
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Wang, Y.; Liu, C.; Hong, T.; Wu, F.; Yu, S.; He, Z.; Mao, W.; Zhou, X. Application of Ammonium Persulfate for Selective Oxidation of Guanines for Nucleic Acid Sequencing. Molecules 2017, 22, 1222.

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