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Molecules 2017, 22(7), 1187; doi:10.3390/molecules22071187

Immunomodulatory Activity of Octenyl Succinic Anhydride Modified Porang (Amorphophallus oncophyllus) Glucomannan on Mouse Macrophage-Like J774.1 Cells and Mouse Primary Peritoneal Macrophages

1
Department of Bioscience, Graduate School of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan
2
Faculty of Agricultural Technology, Gadjah Mada University, Bulaksumur, Yogyakarta 55281, Indonesia
3
Food and Health Sciences Research Center, Ehime University, Matsuyama, Ehime 790-8566, Japan
4
South Ehime Fisheries Research Center, Ehime University, Ainan, Ehime 798-4292, Japan
*
Author to whom correspondence should be addressed.
Received: 20 June 2017 / Revised: 12 July 2017 / Accepted: 12 July 2017 / Published: 15 July 2017
(This article belongs to the Collection Bioactive Compounds)
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Abstract

Porang is a local plant of Indonesia, which has a high content of glucomannan. In this study, porang glucomannan (PG) was esterified with octenyl succinic anhydride (OSA) to enhance emulsion properties to be widely used in food industry. OSA-modified PG (OSA-PG) enhanced the phagocytosis activity of macrophage-like J774.1 cells and mouse peritoneal macrophages. In addition, OSA-PG increased the production of IL-6 and TNF-α by enhancing their gene expression. Immunoblot analysis displayed that OSA-PG tended to activate both nuclear factor-κB and mitogen-activated protein kinase cascades. Treatment of OSA-PG with polymyxin B revealed that cytokine production induced by OSA-PG was not caused by endotoxin contamination. Our findings also indicated that OSA-PG activates macrophages through not only Toll-like receptor (TLR) 4, but another receptor. Overall findings suggested that OSA-PG has a potential as an immunomodulatory food factor by stimulating macrophages. View Full-Text
Keywords: porang glucomannan; octenyl succinic anhydride; macrophage; cytokine; phagocytosis activity; J774.1 cell porang glucomannan; octenyl succinic anhydride; macrophage; cytokine; phagocytosis activity; J774.1 cell
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Gurusmatika, S.; Nishi, K.; Harmayani, E.; Pranoto, Y.; Sugahara, T. Immunomodulatory Activity of Octenyl Succinic Anhydride Modified Porang (Amorphophallus oncophyllus) Glucomannan on Mouse Macrophage-Like J774.1 Cells and Mouse Primary Peritoneal Macrophages. Molecules 2017, 22, 1187.

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