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Molecules 2017, 22(7), 1115; doi:10.3390/molecules22071115

Quality Control of the Root and Rhizome of Helminthostachys zeylanica (Daodi-Ugon) by HPLC Using Quercetin and Ugonins as Markers

1
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan
2
Department of Nursing, Hsin Sheng Junior College of Medical Care and Management, Taoyuan 32544, Taiwan
3
Department of Nursing, Hungkuang University, Taichung 43302, Taiwan
4
Chinese Crude Drug Pharmacy, China Medical University Hospital, Taichung 40402, Taiwan
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 9 June 2017 / Revised: 26 June 2017 / Accepted: 2 July 2017 / Published: 5 July 2017
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Abstract

Daodi-Ugon is the dried root and rhizome of Helminthostachys zeylanica (L.) Hook. and has been used for centuries in the treatment of inflammation, fever, pneumonia, burns, and various disorders. However, the chromatographic methods to determine the phytochemical composition of H. zeylanica have never been reported. This study not only aims to develop a valid high-performance liquid chromatography (HPLC) method and to establish a chromatographic fingerprint for the quality control of H. zeylanica, it also establish the proposed content limits of Quercetin, Ugonin J, and Ugonin M. An HPLC method with a RP18 column (250 × 4.6 mm, 5 μm) was developed for the quantitative analysis of Quercetin, Ugonin J, and Ugonin M in H. zeylanica. A simple gradient of (A) methanol/(B) phosphoric acid in water (5–45 min, 70–80% A; 50–55 min, 80–70% A) was used and 360 nm was selected as the detection wavelength. The average contents and proposed content limits for H. zeylanica were calculated with a t-test and a measurement uncertainty test based on 20 batches of authentic H. zeylanica samples. Limits of detection (LOD), quantification (LOQ), linearity, precision, repeatability, stability, and recovery of the developed method were validated. All of the validation results of quantitative determination and fingerprinting methods were satisfactory. The developed method was then applied to assay the contents of Quercetin, Ugonin J, and Ugonin M and to acquire the fingerprints of all of the collected H. zeylanica samples. At the 99% confidence level, the calculated content limits were 56.45, 112.15, and 277.98 mg/kg for Quercetin, Ugonin J, and Ugonin M, respectively. Those validated HPLC quantitative method, fingerprinting profile, and the proposed content limits of three chemical markers that could be used in the quality control of H. zeylanica in the market. View Full-Text
Keywords: quality control; Helminthostachys zeylanica; Daodi-Ugon; Quercetin; Ugonin J; Ugonin M quality control; Helminthostachys zeylanica; Daodi-Ugon; Quercetin; Ugonin J; Ugonin M
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Wu, K.-C.; Kao, C.-P.; Ho, Y.-L.; Chang, Y.-S. Quality Control of the Root and Rhizome of Helminthostachys zeylanica (Daodi-Ugon) by HPLC Using Quercetin and Ugonins as Markers. Molecules 2017, 22, 1115.

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