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Molecules 2017, 22(7), 1120; doi:10.3390/molecules22071120

Advantages of an Electrochemical Method Compared to the Spectrophotometric Kinetic Study of Peroxidase Inhibition by Boroxine Derivative

1
Department of Chemistry, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and Herzegovina
2
Faculty of Chemistry and Technology, University of Split, Teslina 10, 21 000 Split, Croatia
*
Author to whom correspondence should be addressed.
Received: 29 May 2017 / Accepted: 3 July 2017 / Published: 5 July 2017
(This article belongs to the Special Issue Metallopeptides)
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Abstract

In this study, boroxine derivative (K2[B3O3F4OH]) was tested as an inhibitor of horseradish peroxidase (HRP) by spectrophotometric and electrochemical methods. The activity of horseradish peroxidase was first studied under steady-state kinetic conditions by a spectrophotometric method which required the use of guaiacol as a second substrate to measure guaiacol peroxidation. The results of this method have shown that, by changing the concentration of guaiacol as the literature suggests, a different type of inhibition is observed than when changing the concentration of hydrogen peroxide as the substrate. This suggests that guaiacol interferes with the reaction in some way. The electrochemical method involves direct electron transfer of HRP immobilized in Nafion nanocomposite films on a glassy carbon (GC) electrode, creating a sensor with an electro-catalytic response to the reduction of hydrogen peroxide. The electrochemical method simplifies kinetic assays by removing the requirement of reducing substrates. View Full-Text
Keywords: boroxine derivative; peroxidase; enzyme kinetics; cyclic voltammetry boroxine derivative; peroxidase; enzyme kinetics; cyclic voltammetry
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Ostojić, J.; Herenda, S.; Bešić, Z.; Miloš, M.; Galić, B. Advantages of an Electrochemical Method Compared to the Spectrophotometric Kinetic Study of Peroxidase Inhibition by Boroxine Derivative. Molecules 2017, 22, 1120.

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