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Molecules 2017, 22(7), 1035; doi:10.3390/molecules22071035

Exogenous Feeding of Fructose and Phenylalanine Further Improves Betulin Production in Suspended Betula platyphylla Cells under Nitric Oxide Treatment

1
College of Life Science, Northeast Forestry University, Harbin 150040, China
2
College of Resources and Environmental Science, Heilongjiang University, Harbin 150040, China
*
Author to whom correspondence should be addressed.
Received: 6 May 2017 / Revised: 18 June 2017 / Accepted: 18 June 2017 / Published: 30 June 2017
(This article belongs to the Section Metabolites)
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Abstract

The aim of this study was to assay by NMR the metabolites which contribute to betulin production. 8-day-old suspended birch (Betula platyphylla) cells were treated by sodium nitroprusside (SNP) treatment, an NO donor, and 2-(4-carboxyphenyl)-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide (cPTIO), an NO-specific scavenger. The results showed that betulin production was increased by five times after SNP treatment, similar with that of the control under cPTIO treatment. Forty one metabolites were detected after SNP treatment or cPTIO treatment. Among them, 10 were found to significantly contribute to the differences observed between controls and treated cell culture samples. To validate the contribution of the above 10 metabolites to betulin production, myo-inositol, fructose and phenylalanine based on correlation analysis between the content of 12 metabolites and betulin were used to feed birch suspension cell cultures under SNP treatment. Exogenous feeding of fructose or phenylalanine further enhanced the betulin production under SNP treatment, but myo-inositol had the opposite result. View Full-Text
Keywords: Betula platyphylla; betulin; feeding strategy; metabolic profiling; nitric oxide Betula platyphylla; betulin; feeding strategy; metabolic profiling; nitric oxide
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MDPI and ACS Style

Fan, G.; Nie, T.; Fan, J.S.; Zhan, Y. Exogenous Feeding of Fructose and Phenylalanine Further Improves Betulin Production in Suspended Betula platyphylla Cells under Nitric Oxide Treatment. Molecules 2017, 22, 1035.

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