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Molecules 2017, 22(6), 993; doi:10.3390/molecules22060993

The Molecular Effect of Diagnostic Absorbed Doses from 131I on Papillary Thyroid Cancer Cells In Vitro

1
Department of Neurology, Polish Mother’s Memorial Hospital—Research Institute, 93-338 Lodz, Poland
2
Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, 93-338 Lodz; Poland
3
Department of Endocrinology and Metabolic Diseases, Polish Mother’s Memorial Hospital—Research Institute, 93-338 Lodz, Poland
4
Department of Hematology, Institute of Hematology and Transfusion Medicine, 02-776 Warsaw, Poland
5
Food Science Department, Medical University of Lodz, 90-151 Lodz, Poland
6
Department of Oncological Endocrinology and Nuclear Medicine, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, 02-781 Warsaw, Poland
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Evandro Fei Fang
Received: 14 April 2017 / Revised: 12 June 2017 / Accepted: 13 June 2017 / Published: 15 June 2017
(This article belongs to the Collection New Frontiers in Nucleic Acid Chemistry)
View Full-Text   |   Download PDF [2236 KB, uploaded 15 June 2017]   |  

Abstract

Diagnostic whole-body scan is a standard procedure in patients with thyroid cancer prior to the application of a therapeutic dose of 131I. Unfortunately, administration of the radioisotope in a diagnostic dose may decrease further radioiodine uptake—the phenomenon called “thyroid stunning”. We estimated radiation absorbed dose-dependent changes in genetic material, in particular in the sodium iodide symporter (NIS) gene promoter, and the NIS protein level in a K1 cell line derived from the metastasis of a human papillary thyroid carcinoma exposed to 131I in culture. The different activities applied were calculated to result in absorbed doses of 5, 10 and 20 Gy. Radioiodine did not affect the expression of the NIS gene at the mRNA level, however, we observed significant changes in the NIS protein level in K1 cells. The decrease of the NIS protein level observed in the cells subjected to the lowest absorbed dose was paralleled by a significant increase in 8-oxo-dG concentrations (p < 0.01) and followed by late activation of the DNA repair pathways. Our findings suggest that the impact of 131I radiation on thyroid cells, in the range compared to doses absorbed during diagnostic procedures, is not linear and depends on various factors including the cellular components of thyroid pathology. View Full-Text
Keywords: 131I; genetic material stability; DNA damage; thyroid stunning; papillary thyroid carcinoma 131I; genetic material stability; DNA damage; thyroid stunning; papillary thyroid carcinoma
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Stasiołek, M.; Adamczewski, Z.; Śliwka, P.W.; Puła, B.; Karwowski, B.; Merecz-Sadowska, A.; Dedecjus, M.; Lewiński, A. The Molecular Effect of Diagnostic Absorbed Doses from 131I on Papillary Thyroid Cancer Cells In Vitro. Molecules 2017, 22, 993.

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