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Molecules 2017, 22(2), 215; doi:10.3390/molecules22020215

Evolution of Complex Target SELEX to Identify Aptamers against Mammalian Cell-Surface Antigens

1
Department of Chemistry, Lehman College, The City University of New York, 250 Bedford Park Blvd. West, Bronx, NY 10468, USA
2
Ph.D. Programs in Chemistry and Biochemistry, CUNY Graduate Center 365 Fifth Avenue, New York, NY 10016, USA
3
Ph.D. Program in Molecular, Cellular and Developmental Biology, CUNY Graduate Center 365 Fifth Avenue, New York, NY 10016, USA
Received: 20 December 2016 / Accepted: 24 January 2017 / Published: 30 January 2017
(This article belongs to the Collection New Frontiers in Nucleic Acid Chemistry)
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Abstract

The demand has increased for sophisticated molecular tools with improved detection limits. Such molecules should be simple in structure, yet stable enough for clinical applications. Nucleic acid aptamers (NAAs) represent a class of molecules able to meet this demand. In particular, aptamers, a class of small nucleic acid ligands that are composed of single-stranded modified/unmodified RNA/DNA molecules, can be evolved from a complex library using Systematic Evolution of Ligands by EXponential enrichment (SELEX) against almost any molecule. Since its introduction in 1990, in stages, SELEX technology has itself undergone several modifications, improving selection and broadening the repertoire of targets. This review summarizes these milestones that have pushed the field forward, allowing researchers to generate aptamers that can potentially be applied as therapeutic and diagnostic agents. View Full-Text
Keywords: SELEX; nucleic acid aptamers; cell-surface markers SELEX; nucleic acid aptamers; cell-surface markers
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Mallikaratchy, P. Evolution of Complex Target SELEX to Identify Aptamers against Mammalian Cell-Surface Antigens. Molecules 2017, 22, 215.

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