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Molecules 2017, 22(5), 806; doi:10.3390/molecules22050806

Structure-Activity Relationships of Bioengineered Heparin/Heparan Sulfates Produced in Different Bioreactors

Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW 2052, Australia
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Academic Editors: Giangiacomo Torri and Jawed Fareed
Received: 3 May 2017 / Accepted: 11 May 2017 / Published: 15 May 2017
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Abstract

Heparin and heparan sulfate are structurally-related carbohydrates with therapeutic applications in anticoagulation, drug delivery, and regenerative medicine. This study explored the effect of different bioreactor conditions on the production of heparin/heparan sulfate chains via the recombinant expression of serglycin in mammalian cells. Tissue culture flasks and continuously-stirred tank reactors promoted the production of serglycin decorated with heparin/heparan sulfate, as well as chondroitin sulfate, while the serglycin secreted by cells in the tissue culture flasks produced more highly-sulfated heparin/heparan sulfate chains. The serglycin produced in tissue culture flasks was effective in binding and signaling fibroblast growth factor 2, indicating the utility of this molecule in drug delivery and regenerative medicine applications in addition to its well-known anticoagulant activity. View Full-Text
Keywords: heparin; heparan sulfate; serglycin; proteoglycan; recombinant expression; bioreactor heparin; heparan sulfate; serglycin; proteoglycan; recombinant expression; bioreactor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Kim, H.N.; Whitelock, J.M.; Lord, M.S. Structure-Activity Relationships of Bioengineered Heparin/Heparan Sulfates Produced in Different Bioreactors. Molecules 2017, 22, 806.

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