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Molecules 2017, 22(3), 377; doi:10.3390/molecules22030377

Prevention of Bacterial Contamination of a Silica Matrix Containing Entrapped β-Galactosidase through the Action of Covalently Bound Lysozymes

1
Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Sciences, Jilin University, Changchun 130012, China
2
Informalization Center for Education and Management, Jilin Agricultural University, Changchun 130118, China
3
College of Life Science, Jilin Agricultural University, Changchun 130118, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Roberto Fernandez-Lafuente
Received: 15 January 2017 / Revised: 24 February 2017 / Accepted: 25 February 2017 / Published: 28 February 2017
(This article belongs to the Special Issue Enzyme Immobilization 2016)
View Full-Text   |   Download PDF [9298 KB, uploaded 28 February 2017]   |  

Abstract

β-galactosidase was successfully encapsulated within an amino-functionalised silica matrix using a “fish-in-net” approach and molecular imprinting technique followed by covalent binding of lysozyme via a glutaraldehyde-based method. Transmission electron microscopy (TEM), X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infrared (FTIR) spectroscopy were used to characterise the silica matrix hosting the two enzymes. Both encapsulated β-galactosidase and bound lysozyme exhibited high enzymatic activities and outstanding operational stability in model reactions. Moreover, enzyme activities of the co-immobilised enzymes did not obviously change relative to enzymes immobilised separately. In antibacterial tests, bound lysozyme exhibited 95.5% and 89.6% growth inhibition of Staphylococcus aureus ATCC (American type culture collection) 653 and Escherichia coli ATCC 1122, respectively. In milk treated with co-immobilised enzymes, favourable results were obtained regarding reduction of cell viability and high lactose hydrolysis rate. In addition, when both co-immobilised enzymes were employed to treat milk, high operational and storage stabilities were observed. The results demonstrate that the use of co-immobilised enzymes holds promise as an industrial strategy for producing low lactose milk to benefit people with lactose intolerance. View Full-Text
Keywords: β-galactosidase; lysozyme; encapsulation; covalent binding β-galactosidase; lysozyme; encapsulation; covalent binding
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Li, H.; Li, S.; Tian, P.; Wu, Z.; Li, Z. Prevention of Bacterial Contamination of a Silica Matrix Containing Entrapped β-Galactosidase through the Action of Covalently Bound Lysozymes. Molecules 2017, 22, 377.

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