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Molecules 2017, 22(2), 284; doi:10.3390/molecules22020284

Immobilized Trienzymatic System with Enhanced Stabilization for the Biotransformation of Lactose

Cátedra de Bioquímica, Departamento de Biociencias, Facultad de Química, Universidad de la República, Gral Flores 2124, 11800 Montevideo, Uruguay
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Academic Editors: Roberto Fernández-Lafuente and Derek J. McPhee
Received: 29 November 2016 / Revised: 7 February 2017 / Accepted: 8 February 2017 / Published: 22 February 2017
(This article belongs to the Special Issue Enzyme Immobilization 2016)
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Abstract

The use of ketohexose isomerases is a powerful tool in lactose whey processing, but these enzymes can be very sensitive and expensive. Development of immobilized/stabilized biocatalysts could be a further option to improve the process. In this work, β-galactosidase from Bacillus circulans, l-arabinose (d-galactose) isomerase from Enterococcus faecium, and d-xylose (d-glucose) isomerase from Streptomyces rubiginosus were immobilized individually onto Eupergit C and Eupergit C 250 L. Immobilized activity yields were over 90% in all cases. With the purpose of increasing thermostability of derivatives, two post-immobilization treatments were performed: alkaline incubation to favor the formation of additional covalent linkages, and blocking of excess oxirane groups by reacting with glycine. The greatest thermostability was achieved when alkaline incubation was carried out for 24 h, producing l-arabinose isomerase-Eupergit C derivatives with a half-life of 379 h and d-xylose isomerase-Eupergit C derivatives with a half-life of 554 h at 50 °C. Preliminary assays using immobilized and stabilized biocatalysts sequentially to biotransform lactose at pH 7.0 and 50 °C demonstrated improved performances as compared with soluble enzymes. Further improvements in ketohexose productivities were achieved when the three single-immobilizates were incubated simultaneously with lactose in a mono-reactor system. View Full-Text
Keywords: β-galactosidase; l-arabinose isomerase; d-glucose isomerase; enzyme immobilization; d-tagatose; d-fructose β-galactosidase; l-arabinose isomerase; d-glucose isomerase; enzyme immobilization; d-tagatose; d-fructose
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Torres, P.; Batista-Viera, F. Immobilized Trienzymatic System with Enhanced Stabilization for the Biotransformation of Lactose. Molecules 2017, 22, 284.

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