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Molecules 2017, 22(12), 2108; doi:10.3390/molecules22122108

Recent Advances in Nucleic Acid Targeting Probes and Supramolecular Constructs Based on Pyrene-Modified Oligonucleotides

Institute of Chemical Biology and Fundamental Medicine SB RAS, Acad. Lavrentiev Ave. 8, Novosibirsk 630090, Russia
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Received: 8 October 2017 / Revised: 28 November 2017 / Accepted: 28 November 2017 / Published: 30 November 2017
(This article belongs to the Collection New Frontiers in Nucleic Acid Chemistry)

Abstract

In this review, we summarize the recent advances in the use of pyrene-modified oligonucleotides as a platform for functional nucleic acid-based constructs. Pyrene is of special interest for the development of nucleic acid-based tools due to its unique fluorescent properties (sensitivity of fluorescence to the microenvironment, ability to form excimers and exciplexes, long fluorescence lifetime, high quantum yield), ability to intercalate into the nucleic acid duplex, to act as a π-π-stacking (including anchoring) moiety, and others. These properties of pyrene have been used to construct novel sensitive fluorescent probes for the sequence-specific detection of nucleic acids and the discrimination of single nucleotide polymorphisms (SNPs), aptamer-based biosensors, agents for binding of double-stranded DNAs, and building blocks for supramolecular complexes. Special attention is paid to the influence of the design of pyrene-modified oligonucleotides on their properties, i.e., the structure-function relationships. The perspectives for the applications of pyrene-modified oligonucleotides in biomolecular studies, diagnostics, and nanotechnology are discussed. View Full-Text
Keywords: oligonucleotides; pyrene; fluorescent probes; nucleic acids; SNP detection; aptasensors; G-quadruplexes; TINA; Invader probes; nanoconstructions; supramolecular assemblies oligonucleotides; pyrene; fluorescent probes; nucleic acids; SNP detection; aptasensors; G-quadruplexes; TINA; Invader probes; nanoconstructions; supramolecular assemblies
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Krasheninina, O.A.; Novopashina, D.S.; Apartsin, E.K.; Venyaminova, A.G. Recent Advances in Nucleic Acid Targeting Probes and Supramolecular Constructs Based on Pyrene-Modified Oligonucleotides. Molecules 2017, 22, 2108.

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