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Molecules 2017, 22(12), 2053; doi:10.3390/molecules22122053

Antifungal Activity and Action Mode of Cuminic Acid from the Seeds of Cuminum cyminum L. against Fusarium oxysporum f. sp. Niveum (FON) Causing Fusarium Wilt on Watermelon

1,†
,
1,†
,
1
,
1,2
and
1,2,*
1
Research and Development Center of Biorational Pesticide, Northwest A & F University, Yangling 712100, China
2
Engineering and Research Center of Biological Pesticide of Shaanxi Province, Yangling 712100, China
Joint first authorship.
*
Author to whom correspondence should be addressed.
Received: 31 October 2017 / Revised: 10 November 2017 / Accepted: 21 November 2017 / Published: 30 November 2017
(This article belongs to the Section Natural Products)
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Abstract

In order to develop a novel biofungicide, the antifungal activity and action mode of cuminic acid from the seed of Cuminum cyminum L. against Fusarium oxysporum f. sp. niveum (FON) on watermelon was determined systematically. In this study, the median effective concentration (EC50) value for cuminic acid in inhibiting mycelial growth of FON was 22.53 μg/mL. After treatment with cuminic acid, the mycelial morphology was seriously influenced; cell membrane permeability and glycerol content were increased markedly, but pigment and mycotoxin (mainly fusaric acid) were significantly decreased. Synthesis genes of bikaverin (Bike1, Bike2 and Bike3) and fusaric acid (FUB1, FUB2, FUB3 and FUB4) both were downregulated compared with the control, as confirmed by quantitative RT-PCR. In greenhouse experiments, cuminic acid at all concentrations displayed significant bioactivities against FON. Importantly, significant enhancement of activities of SOD, POD, CAT and decrease of MDA content were observed after in vivo cuminic acid treatment on watermelon leaves. These indicated that cuminic acid not only showed high antifungal activity, but also could enhance the self-defense system of the host plant. Above all, cuminic acid showed the potential as a biofungicide to control FON. View Full-Text
Keywords: watermelon fusarium wilt; p-isopropylbenzoic acid; biofungicide; disease management watermelon fusarium wilt; p-isopropylbenzoic acid; biofungicide; disease management
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MDPI and ACS Style

Sun, Y.; Wang, Y.; Han, L.R.; Zhang, X.; Feng, J.T. Antifungal Activity and Action Mode of Cuminic Acid from the Seeds of Cuminum cyminum L. against Fusarium oxysporum f. sp. Niveum (FON) Causing Fusarium Wilt on Watermelon. Molecules 2017, 22, 2053.

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