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Molecules 2018, 23(1), 124; doi:10.3390/molecules23010124

Single-Labeled Oligonucleotides Showing Fluorescence Changes upon Hybridization with Target Nucleic Acids

Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, Daegu 41566, Korea
Received: 14 December 2017 / Revised: 5 January 2018 / Accepted: 8 January 2018 / Published: 8 January 2018
(This article belongs to the Collection New Frontiers in Nucleic Acid Chemistry)
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Abstract

Sequence-specific detection of nucleic acids has been intensively studied in the field of molecular diagnostics. In particular, the detection and analysis of single-nucleotide polymorphisms (SNPs) is crucial for the identification of disease-causing genes and diagnosis of diseases. Sequence-specific hybridization probes, such as molecular beacons bearing the fluorophore and quencher at both ends of the stem, have been developed to enable DNA mutation detection. Interestingly, DNA mutations can be detected using fluorescently labeled oligonucleotide probes with only one fluorophore. This review summarizes recent research on single-labeled oligonucleotide probes that exhibit fluorescence changes after encountering target nucleic acids, such as guanine-quenching probes, cyanine-containing probes, probes containing a fluorophore-labeled base, and microenvironment-sensitive probes. View Full-Text
Keywords: DNA; nucleic acids chemistry; oligonucleotides; fluorescent probes; single-nucleotide polymorphism; fluorophore DNA; nucleic acids chemistry; oligonucleotides; fluorescent probes; single-nucleotide polymorphism; fluorophore
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Hwang, G.T. Single-Labeled Oligonucleotides Showing Fluorescence Changes upon Hybridization with Target Nucleic Acids. Molecules 2018, 23, 124.

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