Next Article in Journal
Binding Direction-Based Two-Dimensional Flattened Contact Area Computing Algorithm for Protein–Protein Interactions
Next Article in Special Issue
Benzoic Acid Derivatives with Trypanocidal Activity: Enzymatic Analysis and Molecular Docking Studies toward Trans-Sialidase
Previous Article in Journal
Synthesis and Biological Activity of Novel (Z)- and (E)-Verbenone Oxime Esters
Previous Article in Special Issue
Nano-Formulation of Ethambutol with Multifunctional Graphene Oxide and Magnetic Nanoparticles Retains Its Anti-Tubercular Activity with Prospects of Improving Chemotherapeutic Efficacy
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Screening a Natural Product-Based Library against Kinetoplastid Parasites

1
Discovery Biology, Griffith Institute for Drug Discovery, Griffith University, Nathan, Queensland 4111, Australia
2
Natural Product Chemistry, Griffith Institute for Drug Discovery, Griffith University, Nathan, Queensland 4111, Australia
*
Author to whom correspondence should be addressed.
Molecules 2017, 22(10), 1715; https://doi.org/10.3390/molecules22101715
Submission received: 5 September 2017 / Revised: 4 October 2017 / Accepted: 4 October 2017 / Published: 12 October 2017
(This article belongs to the Special Issue Emerging Drug Discovery Approaches against Infectious Diseases)

Abstract

:
Kinetoplastid parasites cause vector-borne parasitic diseases including leishmaniasis, human African trypanosomiasis (HAT) and Chagas disease. These Neglected Tropical Diseases (NTDs) impact on some of the world’s lowest socioeconomic communities. Current treatments for these diseases cause severe toxicity and have limited efficacy, highlighting the need to identify new treatments. In this study, the Davis open access natural product-based library was screened against kinetoplastids (Leishmania donovani DD8, Trypanosoma brucei brucei and Trypanosoma cruzi) using phenotypic assays. The aim of this study was to identify hit compounds, with a focus on improved efficacy, selectivity and potential to target several kinetoplastid parasites. The IC50 values of the natural products were obtained for L. donovani DD8, T. b. brucei and T. cruzi in addition to cytotoxicity against the mammalian cell lines, HEK-293, 3T3 and THP-1 cell lines were determined to ascertain parasite selectivity. Thirty-one compounds were identified with IC50 values of ≤10 µM against the kinetoplastid parasites tested. Lissoclinotoxin E (1) was the only compound identified with activity across all three investigated parasites, exhibiting IC50 values <5 µM. In this study, natural products with the potential to be new chemical starting points for drug discovery efforts for kinetoplastid diseases were identified.

Graphical Abstract

1. Introduction

Trypanosomatida is a group of kinetoplastid protozoa differentiated into the genus Leishmania and Trypanosoma. Parasites belonging to the genus Leishmania are the causative agents of leishmaniasis, while Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense are the causative agents of human African trypanosomiasis (HAT). The other parasite in the genus Trypanosoma is Trypanosoma cruzi, which is responsible for Chagas disease. Around 20 million individuals are infected with kinetoplastid pathogens worldwide leading to 95,000 deaths per year [1]. For Chagas disease and HAT the primary areas of transmission are Latin America and sub-Saharan Africa, respectively [2]. Leishmaniasis is endemic to 98 countries around the globe [3].
The vector for leishmaniasis is the Phlebotomus sand-fly in the Old World and the Lutzomyia sand-fly in the New World [4]; while T. b. gambiense and T. b. rhodesiense are transmitted by the bite of a tsetse fly [5]. T. cruzi is primarily transmitted by triatomine bugs to humans through contact with bug faeces or urine at the site of a bite [6].
The complex multiple host life cycles of these kinetoplastids provides a challenge for drug discovery efforts thus it is preferable that where possible, drugs are able to target several of these life cycle stages, species and in addition, stages of infection. The life cycle of the Leishmania parasite constitutes two key forms; the promastigotes which reside within the gut of the sand fly and the intracellular amastigotes within the mammalian host macrophages [7]. Based on clinical manifestations, leishmaniasis can be differentiated into cutaneous, mucocutaneous and visceral leishmaniasis [8]. T. b. gambiense and T. b. rhodesiense exist as extracellular forms in the tsetse fly as procyclic and metacyclic trypomastigotes. Once injected into the host, they transform into bloodstream trypomastigotes. HAT progresses in two stages: a hemolymphatic stage and a central nervous system (CNS) stage [9]. The T. cruzi life cycle involves three distinct stages; epimastigotes reside within the gut of the triatomine bug where they divide and differentiate into infective metacyclic trypomastigotes in the hindgut. Like leishmaniasis, inside the host they transform into intracellular amastigotes within the infected cell. In Chagas disease; early (acute) stage of the disease may be asymptomatic or display only mild symptoms, while a latter, chronic stage results in cardiac disorders (30%) or digestive disorders (10%), due to the parasite residing in the tissue, or due to associated inflammation in tissue free of the parasite [10]. Kinetoplastid diseases are extremely debilitating and can be fatal without treatment [11].
Existing treatments for leishmaniasis such as miltefosine and amphotericin B deoxycholate [12] have limitations and toxicities associated with them. Current treatments for HAT including pentamidine and eflornithine have variable efficacy at the different stages of the disease and against the different subspecies of T. brucei, and are fraught with severe side effects [13]. For Chagas disease, treatment with the drugs benznidazole and nifurtimox is most effective in the early stages of the disease, but efficacy diminishes with duration of infection [14]. Collectively, the therapies available for kinetoplastid diseases are inadequate, lack efficacy and possess extensive toxicity. Additionally, there is evidence of emerging or potential resistance in leishmaniasis [15], HAT [16], Chagas disease [17] and access to the drugs in remote areas is limited [18,19,20]. Despite the new drug leads currently in clinical trials for leishmaniasis [21,22,23], HAT [24,25,26] and Chagas disease [27], there exists a high attrition rate [28,29] and new molecules with novel mechanisms of action are required.
Natural products have traditionally been used to treat parasitic diseases, primarily through ethnopharmacology approaches [30]. More recent efforts to elucidate the structural and biological properties of the chemical entities within complex anti-parasitic natural product extracts have identified molecules with significant potential for treating NTD’s. For example, recently the antiprotozoal activity of the isolated alkamide dodeca-2E,4E-dienoic acid 4-hydroxy-2-phenyl-ethylamide from Anacyclus pyrethrum roots has been reported against L. donovani, T. b. rhodesiense, T. cruzi and the NF54 strain of Plasmodium falciparum with an IC50 of 4.19 ± 1.64, 2.26 ± 0.18, 1.88 and 3.18 ± 0.20 µM, respectively [31]. Australia has a high level of biodiversity providing an exceptional resource for natural product drug discovery. We have previously reported the identification and biological profiling of compounds originating from plants [32], marine invertebrates [33,34,35] and fungi [36], which possess anti-parasitic activity.
In our continuing search for new anti-parasitic compounds from nature, we report here the identification of several bioactive molecules with activity across multiple life cycle stages of three kinetoplastids, L. donovani DD8 (visceral leishmaniasis: intracellular amastigotes and extracellular promastigotes residing in the gut of sandfly), T. b. brucei (a surrogate species for HAT: bloodstream trypomastigotes) and T. cruzi (Chagas disease: intracellular amastigotes). This is the first evaluation of the unique Davis open access natural product-based library against kinetoplastids which has resulted in the identification of several compounds with novel anti-kinetoplastid activities.

2. Results

2.1. Screening Campaigns and Hit Identification

2.1.1. L. donovani DD8 Promastigote and Intracellular Amastigote Screening

Twenty nine compounds exhibited >70% activity at 16.7 µM against promastigotes with a hit rate of 6.14%. Twelve compounds were active against intracellular amastigotes, with ≥70% inhibition at 20 µM. Of these, 6 compounds showed cytotoxicity against THP-1 cells at 20 µM. Following retest, five compounds exhibited IC50 values of <10 µM against L. donovani DD8 intracellular amastigotes. These five compounds demonstrated comparable activity against both forms (namely promastigote and amastigote), whereas compound (1) (Figure 1) exhibited more potent activity against the promastigotes (IC50: 0.73 ± 0.16 µM) than the amastigotes (IC50: 4.41 ± 0.24 µM). Of these compounds, compound (13) displayed good selectivity of ~12 against the parasite in relation to HEK-293 cells. The Z’ of the intracellular amastigote and promastigote viability assays for primary screen and retest indicated high reproducibility with values of (0.75, 0.72) and (0.91, 0.90), respectively. A Z’ for assays using THP-1 and HEK-293 cells were calculated to be (0.70, 0.69) and (0.78, 0.72), respectively.
The IC50 values for the reference drugs amphotericin B and miltefosine were 0.12 ± 0.01 µM, 0.20 ± 0.02 µM and 3.48 ± 0.26 µM, 2.54 ± 0.57 µM for the promastigote viability and intracellular amastigote assays, respectively (Table 1). The IC50 value for miltefosine was 2.54 ± 0.57 µM in the amastigote assay, which is consistent with data previously reported in the literature (3.1 ± 2.3 µM) [37].

2.1.2. T. b. brucei Screening

A T. b. brucei resazurin viability assay [38] identified 28 compounds with a cut-off of ≥50% activity at 5 µM, indicating a hit rate of 5.93%. Whilst all compounds confirmed activity at the primary screening dose (5 µM), accurate IC50 values could only be determined for 21 compounds. These compounds exhibited IC50 values between 0.05 and 4.84 µM against T. b. brucei, with two compounds showing good selectivity (~18 fold) against HEK-293 cells.
The three reference drugs/compounds used as controls for the T. b. brucei assay were pentamidine, diminazene and puromycin. The IC50 values for pentamidine, diminazene and puromycin were found to be 2.86 ± 1.46 nM, 40.51 ± 12.76 nM and 38.96 ± 6.09 nM, respectively (Table 1) which correlate with previously reported values [39].

2.1.3. T. cruzi Intracellular Amastigote Screening

Using a T. cruzi intracellular amastigote image-based assay [40], 15 compounds with a cut off of ≥50% activity at a concentration of 10 µM were identified, thus a hit rate of 3.17%. In addition to determining the IC50 value against T. cruzi and the 3T3 host cells, compounds were also tested in a HEK-293 assay to determine the activity against a dividing human cell line. The Z’ values obtained for the intracellular amastigote, 3T3 host cell and HEK-293 retest assays were 0.63 ± 0.06, 0.71 ± 0.01 and 0.77 ± 0.03, respectively.
The IC50 values for the reference drugs benznidazole and nifurtimox, as well as puromycin were 3.36 ± 1.52 µM, 0.62 ± 0.10 µM and 1.65 ± 0.35 µM, respectively as shown in Table 1. These correlate with previously published values [40].

2.1.4. Common Activity Against Kinetoplastids

The activity of compounds against multiple kinetoplastid parasites is described in Table 2. Compounds lissoclinotoxin E (1) and spermatinamine (2) demonstrated activity against both L. donovani DD8 forms and T. b. brucei. Seventeen compounds which were active against L. donovani DD8 promastigotes were also active on T. b. brucei, with eight compounds showing activity on both T. b. brucei and T. cruzi (Figure 2A). Compound (1) was active on all three parasites with an IC50 value of <5 µM, however only showed moderate selectivity for T. b. brucei (Figure 2B).

3. Discussion

The drug discovery pipeline for neglected trypanosomatid diseases remains sparse. Drug discovery has had limited success in translating potential drug candidates into viable therapies for HAT, leishmaniasis and Chagas disease. Natural products serve as an attractive alternative source of chemical starting points for drug discovery against kinetoplastid parasites [47,48]. The objective of this work was to identify actives from parallel screening of 472 natural product-derived compounds against the parasites responsible for leishmaniasis, HAT and Chagas disease.
Kinetoplastid pathogens share much of their cellular and molecular biology even though they cause clinically distinctive diseases and are trasmitted by different insect vectors [49]. The parasites in these studies share common molecular targets including trypanothione reductase, pteridine reductase and cysteine protease enzymes, which have been proposed for target-based screening [12,13,14]. Inhibitors have been designed in the past for these enzymes as anti-leishmanials [50,51,52] and anti-trypanosomals [53,54,55]. Fexinidazole is the most advanced oral candidate under development for Chagas disease and HAT, currently in the Phase II and Phase III clicical trials, respectively [56]. This lead compound has also shown potent activity against L. donovani in vitro and in vivo in a visceral leishmaniasis mouse model, illustrating the significant value of screening against multiple kinetoplastids [57].
A target candidate profile (TCP) [18] has been established by Drugs for Neglected Diseases initiative (DNDi) to classify a compound as an active hit against Leishmania, T. b. brucei and T. cruzi. We have used their criteria in terms of efficacy in vitro with an IC50 < 10 µM as the classification of a potential anti-kinetoplastid hit. For L. donovani DD8, less priority has been given to compounds only active on promastigotes alone in comparison to compounds active only on intracellular amastigotes, as intracellular amastigotes represent the pathophysiological relevant form of the disease [37].
Using the SMILE string of active compounds, which was obtained using ChemSpider (http://www.chemspider.com/), previously reported biological activities of compounds were identified using SciFinder (http://www.cas.org/products/scifinder) and ChEMBL (https://www.ebi.ac.uk/chembl/).
Twenty-nine compounds exhibiting >70% activity at 16.7 µM in the L. donovani promastigote viability assay were identified, giving a hit rate of 6.14% (Figure 3) compared to the outcomes from the amastigote screening which identified 6 compounds with a hit rate of 1.27%. We predict that the differences observed in these hit rates are due to several factors including the parasite environment (intracellular vs extracellular); compound permeability and potential effects of the environmental pH on the compound within the parasitophorous vacuole. A similar high hit rate (5.93%) was observed for T. b. brucei which is also an extracellular parasite. Further evidence supports this finding, as 17 compounds were mutually active on L. donovani DD8 promastigotes and T. b. brucei (Figure 1B).
Of the 472 compounds screened, 8 compounds showed activity against both T. b. brucei (extracellular) and T. cruzi intracellular amastigotes (Figure 1B). As the biochemical similarity between T. b. brucei and T. cruzi is much greater than that for Leishmania [58], this may explain why the number of mutual hits between them is much higher than between T. b. brucei and intracellular Leishmania, and T. cruzi and intracellular Leishmania.
Lissoclinotoxin E (1) was the only compound that was pan-active against all three parasites tested with an IC50 value of <5 µM. Lissoclinotoxin E was identified as one of two new dimeric alkaloids [the other is lissoclinotoxin F (11)] which were both initially purified from a didemnid ascidian [59]. Lissoclinotoxin E exhibited an IC50 value of 0.57 ± 0.20 µM and 3.92 ± 0.38 µM against T. b. brucei and T. cruzi, respectively. The antileishmanial activities for compound 1 on promastigotes and intracellular amastigotes were 0.72 ± 0.16 and 4.41 ± 0.24 µM, respectively. Compound 1 exhibited high selectivity for T. b. brucei in relation to HEK 293 cells. The selectivity for HEK-293 and 3T3 cells needs to be confirmed at higher doses for L. donovani DD8 and T. cruzi.
The other dimeric alkaloid, lissoclinotoxin F (11), also showed activity against both forms of the Leishmania parasite (5.51 ± 0.30 and 8.31 ± 0.67 µM, respectively), albeit lower. This is novel activity reported for these compounds against kinetoplastid parasites. Previously, both these compounds have shown interesting activity against PTEN-deficient (PTEN-/-) cell line indicating antitumor activity [59]. This class of compound has shown prominent activity against all three parasites and it could be of interest to further explore the antiprotozoal potential of this class. Analogues can be synthesized which may address any potential cytotoxicity issues.
Spermatinamine (2), isloated from the sponge Pseudoceratina sp. displayed activity on both forms of L. donovani DD8 and T. b. brucei. This compound displayed IC50 values of 6.15 ± 0.05, 11.87 ± 0.56 and 1.00 ± 0.26 µM against L. donovani DD8 intracellular amastigotes, promastigotes and T. b. brucei parasites, respectively. Compound 2 exhibited moderate selectivity for T. b. brucei in relation to HEK 293 cells with no selectivity for L. donovani DD8 intracellular amastigotes and promastigotes. Spermatinamine was originally discovered as a inhibitor of isoprenylcysteine carboxyl methyltransferase (ICMT) [60]. ICMT is a potential target for cancer therapeutics as this enzyme catalyses the final step in post translational modification, where carboxyl methylation of oncogenic proteins takes place. Spematinamine has also been shown to have antibacterial activity against Gram-negative bacteria [61] as well as having antimalarial activity (IC50: 0.23 µM) against P. falciparum (3D7) parasites [62]. Therefore, this may also be the target of this compound in T. b. brucei parasites and warrants further investigation, as this parasite also shares a protein carboxyl methyltransferase [63]. This is the first time that the activity of these compounds has been reported against these parasites and it would be beneficial to identify more kinetoplastid-selective compounds from this class.
On the basis of the information currently available, it seems fair to suggest that thiaplakortone A (3) and thiaplakortone analogue 7 have novel activity against both T. b. brucei and T. cruzi parasites. In addition, gambogic acid (4), mycophenolic acid (5), narciclasine (6), 3,4-dihydro-2H-naphtho[2,3-b][1,4]thiazine-5,10-dione 1,1-dioxide (9) and psammaplysin F (14) have novel activity against T. cruzi parasites.
Thiaplakortone A (3) is a thiazine-derived alkaloid, initially isolated from the marine sponge Plakortis lita [35]. This compound was active against T. b. brucei and T. cruzi with IC50 values of 3.94 ± 0.71 and 4.26 ± 0.65 µM, respectively. Compound 3 exhibited no selectivity for T. b. brucei and T. cruzi. Interestingly, a truncated synthetic thiaplakortone analogue 7, 6-tosyl-2,3-dihydro-[1,4]thiazino[3,2-f]indole-5,9(1H,6H)-dione 4,4-dioxide [64], has also been shown to be active against T. b. brucei and T. cruzi (IC50 of 0.68 ± 0.01 and 3.55 ± 0.38 µM, respectively). Compound 7 has exhibited better selectivity for T. b. brucei and T. cruzi than compound 3, indicating other analogues should be explored to identify selective compounds. Compound 3 was previously reported to have potent activity against both chloroquine-sensitive (3D7) and multi-drug resistant (Dd2) P. falciparum parasites (IC50 of 51, and 6.6 nM, respectively) [35]. Compound 7 has also been tested for activity against 3D7 and Dd2 strains of P. falciparum with reported IC50 values of 546 ± 119 and 509 ± 30.9 nM [64]. The significant difference in plasmodial activity between 3 and 7 may be due to the different thiazine regiochemistry and the lack of an ethylamine chain in 7, compared to the natural product 3 ordifferences in the assay formats used. In vivo testing of thiaplakortone A in a mouse model of malaria infection did not suppress parasitemia with oral administration, possibly due to associated poor bioavailability [65].
Gambogic acid (4) is a xanthonoid isolated from the gamboge resin of the Garcinia hanburyi tree [66] which was demonstrated to have anti-trypanosomal activity against T. b. brucei and T. cruzi (IC50 of 0.27 ± 0.04 and 1.87 ± 0.07 µM, respectively). Compound 4 exhibits moderate selectivity for T. b. brucei in relation to HEK-293 cells. T. b. brucei activity for compound 4 has been previously reported [67] indicating similar values to those obtained for T. b. brucei in this study. The activity in the T. cruzi assay is indicative of host-cell damage as this compound is not selective and has also been shown to be cytotoxic, inducing apoptosis in Jurkat cells through caspase 3 activation and mitochondrial destabilization [68].
Mycophenolic acid (5) is classified as an inosine 5'-monophosphate dehydrogenase inhibitor and the anti-trypanosomal effects of this compound have been identified against T. b. brucei [69] in addition to many other protozoan parasites [70,71]. We observed activity against T. b. brucei (IC50 of 0.51 ± 0.10 µM) which correlates with the previously reported IC50 value [72]. An IC50 value of 1.59 ± 0.03 µM was demonstrated in the T. cruzi assay and showed no selectivity, thus effecting the host cell. To the best of our knowledge this compound has not been tested against T. cruzi previously.
The natural compound narciclasine (6) was first isolated from daffodils, which belong to the Amaryllidaceae plant family [73]. This compound was active against T. b. brucei and T. cruzi (IC50 values of 0.03 ± 0.01 and 0.20 ± 0.01 µM, respectively) and like gambogic acid, has been previously reported with activity against T. b. brucei [67] but not for T. cruzi. Compound 6 has no selectivity for T. b. brucei, or T. cruzi being equally toxic against the mammalian cells. The mechanism of action of this compound suggests that it inhibits protein synthesis by blocking peptide bond formation in Saccharomyces cerevisiae yeast [74]. The compound has anti-viral activity against yellow fever, Japanese encephalitis and dengue fever but due to off target toxicity it has not been tested in in vivo models [75]. Whether a similar mechanism of action is the means by which this compound exerts the effects we observed has not been verified, however as the activity is non-specific, pursuing this compound further is of limited value.
Compounds 9 and 14 showed interesting activity against T. cruzi intracellular amastigotes with IC50 values of 3.82 ± 0.61 and 5.64 ± 0.76 µM, respectively. Compound 9 is a synthetic compound, which exhibits no selectivity for T. cruzi. Compound 14, a bromotyrosine alkaloid named psammaplysin F was isolated from the marine sponge, Hyatella sp [76]. Although IC50 values could not be determined for the host cells or HEK-293 cells for this compound, there was similar activity against the mammalian cell at the concentrations eliciting activity for T. cruzi. This compound has been shown to exhibit antimalarial activity against P. falciparum strains (3D7 and Dd2) with an IC50 value of 0.87 and 1.4 µM, respectively [76].
Compounds 12 (ethyl 4-((diethylamino)methyl)-5-hydroxy-1-(4-methoxyphenyl)-2-methyl-1H-indole-3-carboxylate) and 13 [(E)-1-(2,6-dihydroxy-4-methoxy-3,5-dimethylphenyl)-3-phenylprop-2-en-1-one] showed activity against both intracellular and extracellular forms of L. donovani DD8. Compound 12 and 13 displayed IC50 values of 11.09 ± 0.311 and 5.65 ± 0.26 µM against L. donovani DD8 intracellular amastigotes. Both of these compounds showed no significant activity against either T. b. brucei or T. cruzi. Compound 13 has shown high selectivity for L. donovani DD8 intracellular amastigotes in relation to HEK-293 cells. Compound 13 was previously reported to have moderate activity against both L. donovani (MHOM/SD/62/1S-CL2D) axenic amastigotes and T. b. brucei variant 221 with IC50 values of 16.75 ± 4.35 and 21.11 ± 3.68 µM, respectively [77]. Compound 13 also caused a 96 ± 2% reduction in infection of L. mexicana intracellular amastigotes at 41.89 µM [77]. Previously, it has been reported that compound activity varies based on the geographical location of the species and strains of kinetoplastid parasites [78]. An example in this context is parmomycin, which has negligible activity against L. donovani DD8 when compared to other strains [79]. This may explain the difference of activity observed for compound 13 for Leishmania species; however, differences in assays may also play a role. Compound 13 is a promising hit as it fulfils the TCP criteria set by DNDi for anti-leishmanial drug discovery. It will be interesting to further explore this compound by synthesising analogues and assessing structure activity relationships.
A small percentage (5%) of the library is comprised of commercial or synthetic compounds inspired by natural products. Mefloquine HCl (8), emetine dihydrochloride (10), and chelerythrine chloride (15) which belong to this sub-group were shown to have activity against L. donovani DD8, T. b. brucei and T. cruzi serving as internal controls. The 50% inhibitory concentration observed for mefloquine HCl (8) was 0.62 ± 0.06 and 3.96 ± 0.58 µM for T. b. brucei and T. cruzi, respectively, with moderate selectivity for T. b. brucei. Mefloquine has previously been shown to have activity against T. b. brucei in vitro and in vivo [80] and has a reported EC50 of 6.1 µM against T. cruzi, with a SI of 2 to 3T3 host cells [81]. Herein we found that 3T3 activity was close to that of T. cruzi activity, although higher doses would be needed to confirm the selectivity. We have previously identified this as active against T. cruzi from the Pathogen Box collection of compounds (Duffy et al., 2017). In this study, an IC50 value could also not be determined against host cells.
Emetine dihydrochloride (10) was found to have activity against both T. b. brucei and T. cruzi with an IC50 of 0.05 ± 0.01 and 0.09 ± 0.01 µM, respectively. This compound has shown no selectivity for HEK-293 cells in relation to T. b. brucei and T. cruzi but has shown moderate selectivity for T. cruzi in relation to 3T3 cells, indicating increased cytotoxicity against replicating cells. This compound was previously used as an antiprotozoal drug for the treatment of amoebic liver abscess and invasive intestinal amoebiasis [82]. Its use was discontinued due to severe adverse effects associated with the drug which included cardiotoxic effects [83], severe irritation of oral mucosa, nausea and vomiting [84]. Emetine dihydrochloride has been tested for activity against T. b. brucei [85], in which the IC50 value was 7.9 µM, however it also displayed poor selectivity (3.8) toward MRC5 cells. No data is currently reported in the literature for activity of this compound against T. cruzi.
Chelerythrine chloride (15) exhibited an IC50 of 0.23 ± 0.04 µM against T. b. brucei with good selectivity of >18 fold for HEK-293 cells. Chelerythrine was previously discovered following testing as an oncology drug candidate [86]. Trypanocidal activity has been previously been reported, with an IC50 of 1.3 µM against T. b. brucei [87].

4. Materials and Methods

4.1. Maintenance of Mammalian Cells Lines

THP-1 (human monocytic leukemia) cells obtained from American Type Culture Collection (ATCC®, Manassas, VA, USA) (THP-1 TIB-202™) were maintained in RPMI (1X) Roswell Park Memorial Institute Medium 1640 GlutaMAX™ (Gibco®-Life Technologies, Camarillo, CA, USA) and 10% (v/v) heat inactivated fetal bovine serum (HIFBS) (Hyclone™ ThermoFisher, Melbourne, Australia) at 37 °C in a humidified atmosphere with 95% air and 5% CO2. The cells were passaged every 48 hours to maintain cell density between 2.5 × 105 and 8 × 105 cells/mL.
3T3 host cells (mouse embryo fibroblast; ATCC® CCL92) were maintained in RPMI (Life Technologies, Camarillo ,CA, USA) with no phenol red, supplemented with 10% HIFBS (growth medium).
HEK (human embryonic kidney) 293 cells obtained from the ATCC® (HEK-293 CRL-1573™) were maintained in DMEM (Life Technologies, Camarillo, CA, USA) supplemented with 10% (v/v) HIFBS at 37 °C in a humidified atmosphere of 95% air and 5% CO2. The cells were passaged as they reach 80% confluence.

4.2. Maintainanace of Parasites

L. donovani (MHOM/IN/80 DD8) promastigotes obtained from ATCC®, USA (ATCC® 50212™) were cultivated at 27 °C in Medium 199 (1X) (Gibco®-Life Technologies) supplemented with 100 µM adenosine (CAS Number 58-61-7) (Sigma-Aldrich, Castle Hill, Australia), 23 mM folic acid (CAS Number 59-30-3) (Sigma-Aldrich, Castle Hillcity, Australia), 2 mM L-glutamine 200 mM (Gibco®-Life Technologies) and 1% (v/v) BME vitamin mix 100X (Sigma-Aldrich) and 10% (v/v) HIFBS. The pH of the media was adjusted to 6.8 with 1 M hydrochloric acid (HCl) before being filter sterilised with a cellulose acetate membrane filter, pore size 0.22 µM (Corning® bottle–top vacuum filter system, Oneonta, AL, USA).
T. b. brucei bloodstream form strain 427 (BS427), a surrogate model for human infective species commonly used for the determination of compound activity, was kindly supplied by Dr. Achim Schnaufer, (Institute of Cell Biology, University of Edinburgh, UK) whilst at the Seattle Biomedical Research Institute (Seattle, WA, USA) and was cultivated in HMI-9 media at 37 °C in a humidified atmosphere with 95% air and 5% CO2, according to previosuly published protocols [38,88].
T. cruzi Tulahuen epimastigotes were generously provided by Professor Frederick Buckner (University of Washington, Seattle, WA, USA). The parasites were maintained in liver infusion tryptose (LIT), with preparation as described by Sykes and Avery [40]. Cells were sub-cultured every 3 or 4 days by diluting 1.25–2.5 × 106 cells/mL respectively in to 10 mLof media in 25 cm2 flasks. Cells were grown at 27 °C. Parasites were differentiated into infective trypomastigotes in liquid medium before sub-culturing in 3T3 host cells, as described by Sykes and Avery [40].

4.3. Open Access Compound Library and Assay Plate Preparation

The Davis open access natural product-based library currently consists of 472 distinct compounds, the majority (53%) of which are natural products that have been obtained from Australian natural sources, such as endophytic fungi [89], plants [90], macrofungi [91], and marine invertebrates [92]. Approximately 28% of this library contains semi-synthetic natural product analogues [93], while a smaller percentage (19%) are known commercial drugs or synthetic compounds inspired by natural products.
The Davis open access natural product-based library housed within Compounds Australia (www.compoundsaustralia.com), was dispensed into microtitre plates as 5 mM DMSO solutions. Library compounds were either isolated in quantities ranging from 0.2 mg to >50 mg or purchased from commercial suppliers. The natural product isolation procedures or semi-synthetic studies for the majority of compounds in this unique library have been previously published [89,90,91,92]. All compounds were >95% pure when submitted for storage within Compounds Australia.

4.4. Phenotypic Assays

For the primary screen, compounds were prepared as a stock concentration of 5 mM in 100% DMSO and tested in duplicate points for two independent experiments (n = 2) at final assay concentrations of 20 µM and 16.7 µM for the L. donovani intracellular amastigote and promastigote viability assays, respectively. Primary screening against T. b. brucei was undertaken at 5 µM and for T. cruzi at 10 µM final concentrations. Actives were defined as compounds which exhibited >70% activity at 20 µM and 16.7 µM for L. donovani DD8 intracellular amastigote and promastigote assays, respectively. Compounds exhibiting >50% activity at 5 µM for T. b. brucei and showing ≥50% activity at a concentration of 10 µM for T. cruzi intracellular amastigote were considered active. These compounds were then retested in a 14 point format to determine the IC50 value against the parasite, and against HEK-293, THP-1 and 3T3 cells to determine the selectivity to the parasite. To determine IC50 values, 14 point dose response curves with top concentrations of 20 µM and 16.7 µM were used for the L. donovani DD8 intracellular amastigote and promastigote viability assays, respectively. A top dose of 20 µM was used in the T. b. brucei assay and 18.3 µM for T.cruzi assay, in 14 point dose repsonse curves as described.

4.4.1. L. donovani DD8 Intracellular Amastigote Assay

A high content, high-throughput imaging assay was used to identify compounds active against L. donovani DD8 intracellular amastigotes as described previosuly [88].

4.4.2. L. donovani DD8 Promastigote Viability Assay

This assay was established to assess the activity of the compound library on the extracellular form of the parasites. An initial cell density of 1 × 105 parasites/mL was inoculated into a 75 cm2 flask (in a total of 30 mL of M199 medium + 10% (v/v) HIFBS) and incubated at 27 °C. After 96 h the parasites reached the mid-log phase and were seeded in black, clear bottom 384-well plates (Greiner-bio-one®, Monroe, UT, USA) at a concentration of 5 × 105 parasites/mL in a volume of 55 µL using a Bravo automated liquid handling platform (Agilent, Santa Clara, CA, USA). Compound stocks were diluted 1:25 in M199 media without HIFBS using a dilution plate (Greiner-bio-one®, Monroe, UT, USA). The compounds were dispensed in 5 µL using a Bravo and parasites incubated for an additional 68 h at 27 °C. Resazurin (Cayman Chemicals®, Ann Arbor, MI, USA) at 0.142 mM final assay concentration was then dispensed into plates at a volume of 10 µL/well using a Multidrop™ 384 Reagent Dispenser (Thermo Scientific®, Newington, NH, USA) and incubated at 27 °C. The plates were read after 4 h on an EnVision™ Multilabel plate reader (PerkinElmer, Waltham, MA, USA) using fluorometry with an excitation of 530 nm and emission 590 nm. For the L. donovani DD8 promastigote viability assay, 1 µM of amphotericin B was used as positive control and 0.4% DMSO as negative control.

4.4.3. T. b. brucei Resazurin Viability Assay

The efficacy of compounds against T. b. brucei bloodstream form was evaluated using a resazurin-based assay, previously described in the literature [88].

4.4.4. T. cruzi Intracellular Amastigote Assay

The efficacy of compounds against T. cruzi was undertaken using a phenotypic, high-throughput image-based assay as previously described [40].

4.4.5. HEK-293 Resazurin Viability Assay

Cytotoxicity testing was undertaken as previously described [88].

5. Conclusions

The present study identifies several compounds with novel anti-kinetoplastid activities from phenotypic screening of 472 distinct compounds from the Davis open access natural product-based library. Compound 1 displayed in vitro activity against, L. donovani DD8, T. b. brucei and T. cruzi with good selectivity against T. b. brucei. Further exploration of the activity of analogues of this compound is warranted to improve anti-parasitic selectivity. Target identification for the most promising compounds will support the future development of pan-active treatments against kinetoplastids. Further compounds from this collection have potential as new chemical starting points for drug discovery efforts against one or more of the parasites tested.

Acknowledgments

B.Z. is the recipient of Griffith University International Postgraduate Research Scholarship (GUIPRS) and Griffith University Postgraduate Research Scholarship (GUPRS). This work was supported by NHMRC project grant APP1067728 and ARC grant LP140100560 awarded to V.M.A. The authors thank and acknowledge the contributions of Mahreen Rasheed, Jemima Wixted, Elouise Gaylard and Kate Parsons for their assistance with parasite and mammalian cell culturing and assays. Compounds Australia (www.compoundsaustralia.com) processed the Davis open access natural product library, which forms part of the Open Access Compound Collection. R.A.D. acknowledges the NatureBank biota repository, (www.griffith.edu.au/gridd) from which many of the compounds in the Davis open access natural product library were derived.

Author Contributions

B.Z. conceived, designed, collected the data and literature for the manuscript. B.Z., M.L.S. and A.J.J. performed the experiments. T.B.S., A.J.J. and M.L.S. assisted in the experiment and data analysis. T.B.S., R.A.D. and V.M.A. supervised the study. B.Z., R.A.D. and V.M.A. wrote the manuscript and all authors reviewed the manuscript. All authors have read and approved the final version of the manuscript.

Conflicts of Interest

The authors declare no conflict of interests.

References

  1. World Health Organization (WHO). Investing to Overcome the Global Impact of Neglected Tropical Diseases: Third Who Report on negLected Tropical Diseases 2015; World Health Organization: Geneva, Switzerland, 2015; Volume 3. [Google Scholar]
  2. Molyneux, D.H.; Savioli, L.; Engel, D. Neglected tropical diseases: Progress towards addressing the chronic pandemic. Lancet 2017, 389, 312–325. [Google Scholar] [CrossRef]
  3. Borghi, S.M.; Fattori, V.; Conchon-Costa, I.; Pinge-Filho, P.; Pavanelli, W.R.; Verri, W.A., Jr. Leishmania infection: Painful or painless? Parasitol. Res. 2017, 116, 465–475. [Google Scholar] [CrossRef] [PubMed]
  4. Herwaldt, B.L. Leishmaniasis. Lancet 1999, 354, 1191–1199. [Google Scholar] [CrossRef]
  5. Van Den Abbeele, J.; Claes, Y.; Van Bockstaele, D.; Le Ray, D.; Coosemans, M. Trypanosoma brucei spp. Development in the tsetse fly: Characterization of the post-mesocyclic stages in the foregut and proboscis. Parasitology 1999, 118, 469–478. [Google Scholar] [CrossRef] [PubMed]
  6. Garcia, E.; Azambuja, P. Development and interactions of Trypanosoma cruzi within the insect vector. Parasitol. Today 1991, 7, 240–244. [Google Scholar] [CrossRef]
  7. Handman, E.; Bullen, D.V. Interaction of Leishmania with the host macrophage. Trends Parasitol. 2002, 18, 332–334. [Google Scholar] [CrossRef]
  8. Alvar, J.; Velez, I.D.; Bern, C.; Herrero, M.; Desjeux, P.; Cano, J.; Jannin, J.; den Boer, M. Leishmaniasis worldwide and global estimates of its incidence. PLoS ONE 2012, 7, e35671. [Google Scholar] [CrossRef] [PubMed]
  9. Keating, J.; Yukich, J.O.; Sutherland, C.S.; Woods, G.; Tediosi, F. Human african trypanosomiasis prevention, treatment and control costs: A systematic review. Acta Trop. 2015, 150, 4–13. [Google Scholar] [CrossRef] [PubMed]
  10. Bonney, K.M.; Engman, D.M. Autoimmune pathogenesis of chagas heart disease: Looking back, looking ahead. Am. J. Pathol. 2015, 185, 1537–1547. [Google Scholar] [CrossRef] [PubMed]
  11. McCall, L.-I.; McKerrow, J.H. Determinants of disease phenotype in trypanosomatid parasites. Trends Parasitol. 2014, 30, 342–349. [Google Scholar] [CrossRef] [PubMed]
  12. Sundar, S.; Chakravarty, J. An update on pharmacotherapy for leishmaniasis. Expert Opin. Pharmacother. 2015, 16, 237–252. [Google Scholar] [CrossRef] [PubMed]
  13. Jones, A.J.; Avery, V.M. Future treatment options for human african trypanosomiasis. Expert Rev. Anti-Infect. Ther. 2015, 13, 1429–1432. [Google Scholar] [CrossRef] [PubMed]
  14. Bermudez, J.; Davies, C.; Simonazzi, A.; Real, J.P.; Palma, S. Current drug therapy and pharmaceutical challenges for chagas disease. Acta Trop. 2016, 156, 1–16. [Google Scholar] [CrossRef] [PubMed]
  15. Mondelaers, A.; Sanchez-Cañete, M.P.; Hendrickx, S.; Eberhardt, E.; Garcia-Hernandez, R.; Lachaud, L.; Cotton, J.; Sanders, M.; Cuypers, B.; Imamura, H. Genomic and molecular characterization of miltefosine resistance in Leishmania infantum strains with either natural or acquired resistance through experimental selection of intracellular amastigotes. PLoS ONE 2016, 11, e0154101. [Google Scholar] [CrossRef] [PubMed]
  16. Barrett, M.P.; Vincent, I.M.; Burchmore, R.J.; Kazibwe, A.J.; Matovu, E. Drug resistance in human african trypanosomiasis. Future Med. 2011, 6, 1037–1047. [Google Scholar] [CrossRef] [PubMed]
  17. Campos, M.C.O.; Leon, L.L.; Taylor, M.C.; Kelly, J.M. Benznidazole-resistance in trypanosoma cruzi: Evidence that distinct mechanisms can act in concert. Mol. Biochem. Parasitol. 2014, 193, 17–19. [Google Scholar] [CrossRef] [PubMed]
  18. Don, R.; Ioset, J.R. Screening strategies to identify new chemical diversity for drug development to treat kinetoplastid infections. Parasitology 2014, 141, 140–146. [Google Scholar] [CrossRef] [PubMed]
  19. Brun, R.; Blum, J.; Chappuis, F.; Burri, C. Human african trypanosomiasis. Lancet 2010, 375, 148–159. [Google Scholar] [CrossRef]
  20. Aronson, N.E. Addressing a clinical challenge: Guidelines for the diagnosis and treatment of leishmaniasis. BMC Med. 2017, 15, 76. [Google Scholar] [CrossRef] [PubMed]
  21. Bhuniya, D.; Mukkavilli, R.; Shivahare, R.; Launay, D.; Dere, R.T.; Deshpande, A.; Verma, A.; Vishwakarma, P.; Moger, M.; Pradhan, A.; et al. Aminothiazoles: Hit to lead development to identify antileishmanial agents. Eur. J. Med. Chem. 2015, 102, 582–593. [Google Scholar] [CrossRef] [PubMed]
  22. Mowbray, C.E.; Braillard, S.; Speed, W.; Glossop, P.A.; Whitlock, G.A.; Gibson, K.R.; Mills, J.E.; Brown, A.D.; Gardner, J.M.; Cao, Y.; et al. Novel amino-pyrazole ureas with potent in vitro and in vivo antileishmanial activity. J. Med. Chem. 2015, 58, 9615–9624. [Google Scholar] [CrossRef] [PubMed]
  23. Zulfiqar, B.; Shelper, T.B.; Avery, V.M. Leishmaniasis drug discovery: Recent progress and challenges in assay development. Drug Discov. Today 2017. [Google Scholar] [CrossRef] [PubMed]
  24. Torreele, E.; Trunz, B.B.; Tweats, D.; Kaiser, M.; Brun, R.; Mazue, G.; Bray, M.A.; Pecoul, B. Fexinidazole—A new oral nitroimidazole drug candidate entering clinical development for the treatment of sleeping sickness. PLoS Negl. Trop. Dis. 2010, 4, e923. [Google Scholar] [CrossRef] [PubMed]
  25. Jacobs, R.T.; Nare, B.; Wring, S.A.; Orr, M.D.; Chen, D.; Sligar, J.M.; Jenks, M.X.; Noe, R.A.; Bowling, T.S.; Mercer, L.T. Scyx-7158, an orally-active benzoxaborole for the treatment of stage 2 human african trypanosomiasis. PLoS Negl. Trop. Dis. 2011, 5, e1151. [Google Scholar] [CrossRef] [PubMed]
  26. Kaiser, M.; Bray, M.A.; Cal, M.; Bourdin Trunz, B.; Torreele, E.; Brun, R. Antitrypanosomal activity of fexinidazole, a new oral nitroimidazole drug candidate for treatment of sleeping sickness. Antimicrob. Agents Chemother. 2011, 55, 5602–5608. [Google Scholar] [CrossRef] [PubMed]
  27. Field, M.C.; Horn, D.; Fairlamb, A.H.; Ferguson, M.A.; Gray, D.W.; Read, K.D.; De Rycker, M.; Torrie, L.S.; Wyatt, P.G.; Wyllie, S.; et al. Anti-trypanosomatid drug discovery: An ongoing challenge and a continuing need. Nat. Rev. Microbiol. 2017, 15, 217–231. [Google Scholar] [CrossRef] [PubMed]
  28. Kola, I.; Landis, J. Can the pharmaceutical industry reduce attrition rates? Nat. Rev. Drug Discov. 2004, 3, 711–716. [Google Scholar] [CrossRef] [PubMed]
  29. Nwaka, S.; Besson, D.; Ramirez, B.; Maes, L.; Matheeussen, A.; Bickle, Q.; Mansour, N.R.; Yousif, F.; Townson, S.; Gokool, S.; et al. Integrated dataset of screening hits against multiple neglected disease pathogens. PLoS Negl. Trop. Dis. 2011, 5, e1412. [Google Scholar] [CrossRef] [PubMed]
  30. Silva, J.R.; Ramos Ade, S.; Machado, M.; de Moura, D.F.; Neto, Z.; Canto-Cavalheiro, M.M.; Figueiredo, P.; do Rosario, V.E.; Amaral, A.C.; Lopes, D. A review of antimalarial plants used in traditional medicine in communities in portuguese-speaking countries: Brazil, mozambique, cape verde, guinea-bissau, sao tome and principe and angola. Mem. Inst. Oswaldo Cruz 2011, 106, 142–158. [Google Scholar] [CrossRef] [PubMed]
  31. Althaus, J.B.; Malyszek, C.; Kaiser, M.; Brun, R.; Schmidt, T.J. Alkamides from Anacyclus pyrethrum l. and their in vitro antiprotozoal activity. Molecules 2017, 22, 796. [Google Scholar] [CrossRef] [PubMed]
  32. Yang, X.; Feng, Y.; Duffy, S.; Avery, V.M.; Camp, D.; Quinn, R.J.; Davis, R.A. A new quinoline epoxide from the australian plant Drummondita calida. Planta Med. 2011, 77, 1644–1647. [Google Scholar] [CrossRef] [PubMed]
  33. Davis, R.A.; Sykes, M.; Avery, V.M.; Camp, D.; Quinn, R.J. Convolutamines I and J, antitrypanosomal alkaloids from the bryozoan Amathia tortusa. Bioorg. Med. Chem. 2011, 19, 6615–6619. [Google Scholar] [CrossRef] [PubMed]
  34. Feng, Y.; Davis, R.A.; Sykes, M.L.; Avery, V.M.; Quinn, R.J. Iotrochamides A and B, antitrypanosomal compounds from the Australian marine sponge Iotrochota sp. Bioorg. Med. Chem. Lett. 2012, 22, 4873–4876. [Google Scholar] [CrossRef] [PubMed]
  35. Davis, R.A.; Duffy, S.; Fletcher, S.; Avery, V.M.; Quinn, R.J. Thiaplakortones A-D: Antimalarial thiazine alkaloids from the Australian marine sponge Plakortis lita. J. Org. Chem. 2013, 78, 9608–9613. [Google Scholar] [CrossRef] [PubMed]
  36. Choomuenwai, V.; Beattie, K.D.; Healy, P.C.; Andrews, K.T.; Fechner, N.; Davis, R.A. Entonalactams A–C: Isoindolinone derivatives from an Australian rainforest fungus belonging to the genus Entonaema. Phytochemistry 2015, 117, 10–16. [Google Scholar] [CrossRef] [PubMed]
  37. Siqueira-Neto, J.L.; Moon, S.; Jang, J.; Yang, G.; Lee, C.; Moon, H.K.; Chatelain, E.; Genovesio, A.; Cechetto, J.; Freitas-Junior, L.H. An image-based high-content screening assay for compounds targeting intracellular Leishmania donovani amastigotes in human macrophages. PLoS Negl. Trop. Dis. 2012, 6, e1671. [Google Scholar] [CrossRef] [PubMed]
  38. Sykes, M.L.; Avery, V.M. Development of an alamar blue viability assay in 384-well format for high throughput whole cell screening of Trypanosoma brucei brucei bloodstream form strain 427. Am. J. Trop. Med. Hyg. 2009, 81, 665–674. [Google Scholar] [CrossRef] [PubMed]
  39. Jones, A.J.; Kaiser, M.; Avery, V.M. Identification and characterization of fty720 for the treatment of human african trypanosomiasis. Antimicrob. Agents Chemother. 2015, 60, 1859–1861. [Google Scholar] [CrossRef] [PubMed]
  40. Sykes, M.L.; Avery, V.M. Development and application of a sensitive, phenotypic, high-throughput image-based assay to identify compound activity against Trypanosoma cruzi amastigotes. Int. J. Parasitol. Drugs Drug Resist. 2015, 5, 215–228. [Google Scholar] [CrossRef] [PubMed]
  41. Roberts, C.; McLeod, R.; Rice, D.; Ginger, M.; Chance, M.L.; Goad, L.J. Fatty acid and sterol metabolism: Potential antimicrobial targets in apicomplexan and trypanosomatid parasitic protozoa. Mol. Biochem. Parasitol. 2003, 126, 129–142. [Google Scholar] [CrossRef]
  42. Freitas-Junior, L.H.; Chatelain, E.; Kim, H.A.; Siqueira-Neto, J.L. Visceral leishmaniasis treatment: What do we have, what do we need and how to deliver it? Int. J. Parasitol. Drugs Drug Resist. 2012, 2, 11–19. [Google Scholar] [CrossRef] [PubMed]
  43. Luque-Ortega, J.R.; Rivas, L. Miltefosine (hexadecylphosphocholine) inhibits cytochrome c oxidase in Leishmania donovani promastigotes. Antimicrob. Agents Chemother. 2007, 51, 1327–1332. [Google Scholar] [CrossRef] [PubMed]
  44. Barrett, M.P.; Boykin, D.W.; Brun, R.; Tidwell, R.R. Human african trypanosomiasis: Pharmacological re-engagement with a neglected disease. Br. J. Pharmacol. 2007, 152, 1155–1171. [Google Scholar] [CrossRef] [PubMed]
  45. Rajao, M.A.; Furtado, C.; Alves, C.L.; Passos-Silva, D.G.; de Moura, M.B.; Schamber-Reis, B.L.; Kunrath-Lima, M.; Zuma, A.A.; Vieira-da-Rocha, J.P.; Garcia, J.B.F.; et al. Unveiling benznidazole’s mechanism of action through overexpression of DNA repair proteins in Trypanosoma cruzi. Environ. Mol. Mutagen. 2014, 55, 309–321. [Google Scholar] [CrossRef] [PubMed]
  46. Boiani, M.; Piacenza, L.; Hernández, P.; Boiani, L.; Cerecetto, H.; González, M.; Denicola, A. Mode of action of nifurtimox and n-oxide-containing heterocycles against Trypanosoma cruzi: Is oxidative stress involved? Biochem. Pharmacol. 2010, 79, 1736–1745. [Google Scholar] [CrossRef] [PubMed]
  47. Annang, F.; Pérez-Moreno, G.; García-Hernández, R.; Cordon-Obras, C.; Martín, J.; Tormo, J.; Rodríguez, L.; De Pedro, N.; Gómez-Pérez, V.; Valente, M. High-throughput screening platform for natural product–based drug discovery against 3 neglected tropical diseases: Human african trypanosomiasis, leishmaniasis, and chagas disease. J. Biomol. Screen. 2015, 20, 82–91. [Google Scholar] [CrossRef] [PubMed]
  48. Jones, A.J.; Grkovic, T.; Sykes, M.L.; Avery, V.M. Trypanocidal activity of marine natural products. Mar. Drugs 2013, 11, 4058–4082. [Google Scholar] [CrossRef] [PubMed]
  49. Stuart, K.; Brun, R.; Croft, S.; Fairlamb, A.; Gurtler, R.E.; McKerrow, J.; Reed, S.; Tarleton, R. Kinetoplastids: Related protozoan pathogens, different diseases. J. Clin. Investig. 2008, 118, 1301–1310. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  50. Baiocco, P.; Poce, G.; Alfonso, S.; Cocozza, M.; Porretta, G.C.; Colotti, G.; Biava, M.; Moraca, F.; Botta, M.; Yardley, V.; et al. Inhibition of Leishmania infantum trypanothione reductase by azole-based compounds: A comparative analysis with its physiological substrate by x-ray crystallography. ChemMedChem 2013, 8, 1175–1183. [Google Scholar] [CrossRef] [PubMed]
  51. Corona, P.; Gibellini, F.; Cavalli, A.; Saxena, P.; Carta, A.; Loriga, M.; Luciani, R.; Paglietti, G.; Guerrieri, D.; Nerini, E.; et al. Structure-based selectivity optimization of piperidine-pteridine derivatives as potent Leishmania pteridine reductase inhibitors. J. Med. Chem. 2012, 55, 8318–8329. [Google Scholar] [CrossRef] [PubMed]
  52. Schroder, J.; Noack, S.; Marhofer, R.J.; Mottram, J.C.; Coombs, G.H.; Selzer, P.M. Identification of semicarbazones, thiosemicarbazones and triazine nitriles as inhibitors of Leishmania mexicana cysteine protease cpb. PLoS ONE 2013, 8, e77460. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  53. Cavalli, A.; Lizzi, F.; Bongarzone, S.; Brun, R.; Luise Krauth-Siegel, R.; Bolognesi, M.L. Privileged structure-guided synthesis of quinazoline derivatives as inhibitors of trypanothione reductase. Bioorg. Med. Chem. Lett. 2009, 19, 3031–3035. [Google Scholar] [CrossRef] [PubMed]
  54. Mpamhanga, C.P.; Spinks, D.; Tulloch, L.B.; Shanks, E.J.; Robinson, D.A.; Collie, I.T.; Fairlamb, A.H.; Wyatt, P.G.; Frearson, J.A.; Hunter, W.N.; et al. One scaffold, three binding modes: Novel and selective pteridine reductase 1 inhibitors derived from fragment hits discovered by virtual screening. J. Med. Chem. 2009, 52, 4454–4465. [Google Scholar] [CrossRef] [PubMed]
  55. Breuning, A.; Degel, B.; Schulz, F.; Buchold, C.; Stempka, M.; Machon, U.; Heppner, S.; Gelhaus, C.; Leippe, M.; Leyh, M.; et al. Michael acceptor based antiplasmodial and antitrypanosomal cysteine protease inhibitors with unusual amino acids. J. Med. Chem. 2010, 53, 1951–1963. [Google Scholar] [CrossRef] [PubMed]
  56. DNDi. Fexinidazole (hat). Available online: Http://www.Dndi.Org/diseases-projects/portfolio/fexinidazole/ (accessed on 17 May 2017).
  57. Wyllie, S.; Patterson, S.; Stojanovski, L.; Simeons, F.R.; Norval, S.; Kime, R.; Read, K.D.; Fairlamb, A.H. The anti-trypanosome drug fexinidazole shows potential for treating visceral leishmaniasis. Sci. Transl. Med. 2012, 4, 119re1. [Google Scholar] [CrossRef] [PubMed]
  58. Marr, J.; Muller, M. Biochemistry and Molecular Biology of Parasites; Academic Press: Cambridge, MA, USA, 1995. [Google Scholar]
  59. Davis, R.A.; Sandoval, I.T.; Concepcion, G.P.; da Rocha, R.M.; Ireland, C.M. Lissoclinotoxins e and f, novel cytotoxic alkaloids from a Philippine Didemnid ascidian. Tetrahedron 2003, 59, 2855–2859. [Google Scholar] [CrossRef]
  60. Buchanan, M.S.; Carroll, A.R.; Fechner, G.A.; Boyle, A.; Simpson, M.M.; Addepalli, R.; Avery, V.M.; Hooper, J.N.; Su, N.; Chen, H.; et al. Spermatinamine, the first natural product inhibitor of isoprenylcysteine carboxyl methyltransferase, a new cancer target. Bioorg. Med. Chem. Lett. 2007, 17, 6860–6863. [Google Scholar] [CrossRef] [PubMed]
  61. Yin, S.; Davis, R.A.; Shelper, T.; Sykes, M.L.; Avery, V.M.; Elofsson, M.; Sundin, C.; Quinn, R.J. Pseudoceramines A–D, new antibacterial bromotyrosine alkaloids from the marine sponge Pseudoceratina sp. Org. Biomol. Chem. 2011, 9, 6755–6760. [Google Scholar] [CrossRef] [PubMed]
  62. Choomuenwai, V.; Schwartz, B.D.; Beattie, K.D.; Andrews, K.T.; Khokhar, S.; Davis, R.A. The discovery, synthesis and antimalarial evaluation of natural product-based polyamine alkaloids. Tetrahedron Lett. 2013, 54, 5188–5191. [Google Scholar] [CrossRef]
  63. Buckner, F.S.; Kateete, D.P.; Lubega, G.W.; Van Voorhis, W.C.; Yokoyama, K. Trypanosoma brucei prenylated-protein carboxyl methyltransferase prefers farnesylated substrates. Biochem. J. 2002, 367, 809–816. [Google Scholar] [CrossRef] [PubMed]
  64. Schwartz, B.D.; Coster, M.J.; Skinner-Adams, T.S.; Andrews, K.T.; White, J.M.; Davis, R.A. Synthesis and antiplasmodial evaluation of analogues based on the tricyclic core of thiaplakortones A-D. Mar. Drugs 2015, 13, 5784–5795. [Google Scholar] [CrossRef] [PubMed]
  65. Schwartz, B.D.; Skinner-Adams, T.S.; Andrews, K.T.; Coster, M.J.; Edstein, M.D.; MacKenzie, D.; Charman, S.A.; Koltun, M.; Blundell, S.; Campbell, A.; et al. Synthesis and antimalarial evaluation of amide and urea derivatives based on the thiaplakortone a natural product scaffold. Org. Biomol. Chem. 2015, 13, 1558–1570. [Google Scholar] [CrossRef] [PubMed]
  66. Zhang, H.-Z.; Kasibhatla, S.; Wang, Y.; Herich, J.; Guastella, J.; Tseng, B.; Drewe, J.; Cai, S.X. Discovery, characterization and sar of gambogic acid as a potent apoptosis inducer by a hts assay. Bioorg. Med. Chem. 2004, 12, 309–317. [Google Scholar] [CrossRef] [PubMed]
  67. Mackey, Z.B.; Baca, A.M.; Mallari, J.P.; Apsel, B.; Shelat, A.; Hansell, E.J.; Chiang, P.K.; Wolff, B.; Guy, K.R.; Williams, J.; et al. Discovery of trypanocidal compounds by whole cell hts of Trypanosoma brucei. Chem. Biol. Drug Des. 2006, 67, 355–363. [Google Scholar] [CrossRef] [PubMed]
  68. Wu, X.; Cao, S.; Goh, S.; Hsu, A.; Tan, B.K. Mitochondrial destabilisation and caspase-3 activation are involved in the apoptosis of jurkat cells induced by gaudichaudione a, a cytotoxic xanthone. Planta Med. 2002, 68, 198–203. [Google Scholar] [CrossRef] [PubMed]
  69. Bessho, T.; Morii, S.; Kusumoto, T.; Shinohara, T.; Noda, M.; Uchiyama, S.; Shuto, S.; Nishimura, S.; Djikeng, A.; Duszenko, M. Characterization of the novel Trypanosoma brucei inosine 5′-monophosphate dehydrogenase. Parasitology 2013, 140, 735–745. [Google Scholar] [CrossRef] [PubMed]
  70. Cao, S.; Aboge, G.O.; Terkawi, M.A.; Zhou, M.; Luo, Y.; Yu, L.; Li, Y.; Goo, Y.; Kamyingkird, K.; Masatani, T. Cloning, characterization and validation of inosine 5′-monophosphate dehydrogenase of Babesia gibsoni as molecular drug target. Parasitol. Int. 2013, 62, 87–94. [Google Scholar] [CrossRef] [PubMed]
  71. Sullivan, W.J.; Dixon, S.E.; Li, C.; Striepen, B.; Queener, S.F. Imp dehydrogenase from the protozoan parasite Toxoplasma gondii. Antimicrob. Agents Chemother. 2005, 49, 2172–2179. [Google Scholar] [CrossRef] [PubMed]
  72. Suganuma, K.; Sarwono, A.E.; Mitsuhashi, S.; Jakalski, M.; Okada, T.; Nthatisi, M.; Yamagishi, J.; Ubukata, M.; Inoue, N. Mycophenolic acid and its derivatives as potential chemotherapeutic agents targeting inosine monophosphate dehydrogenase in Trypanosoma congolense. Antimicrob. Agents Chemother. 2016, 60, 4391–4393. [Google Scholar] [CrossRef] [PubMed]
  73. Ceriotti, G. Narciclasine: An antimitotic substance from narcissus bulbs. Nature 1967, 213, 595–596. [Google Scholar] [CrossRef] [PubMed]
  74. Carrasco, L.; Fresno, M.; Vazquez, D. Narciclasine: An antitumour alkaloid which blocks peptide bond formation by eukaryotic ribosomes. FEBS Lett. 1975, 52, 236–239. [Google Scholar] [CrossRef]
  75. Gabrielsen, B.; Monath, T.P.; Huggins, J.W.; Kirsi, J.J.; Hollingshead, M.; Shannon, W.M.; Pettit, G.R. Activity of selected Amaryllidaceae constituents and related synthetic substances against medically important RNA viruses. In Natural Products as Antiviral Agents; Springer: New York, NY, USA, 1992; pp. 121–135. [Google Scholar]
  76. Yang, X.; Davis, R.A.; Buchanan, M.S.; Duffy, S.; Avery, V.M.; Camp, D.; Quinn, R.J. Antimalarial bromotyrosine derivatives from the australian marine sponge Hyattella sp. J. Nat. Prod. 2010, 73, 985–987. [Google Scholar] [CrossRef] [PubMed]
  77. Salem, M.M.; Werbovetz, K.A. Antiprotozoal compounds from Psorothamnus polydenius. J. Nat. Prod. 2005, 68, 108–111. [Google Scholar] [CrossRef] [PubMed]
  78. Croft, S.L.; Yardley, V.; Kendrick, H. Drug sensitivity of Leishmania species: Some unresolved problems. Trans. R. Soc. Trop. Med. Hyg. 2002, 96, S127–S129. [Google Scholar] [CrossRef]
  79. Neal, R.; Allen, S.; McCoy, N.; Olliaro, P.; Croft, S. The sensitivity of Leishmania species to aminosidine. J. Antimicrob. Chemother. 1995, 35, 577–584. [Google Scholar] [CrossRef] [PubMed]
  80. Otigbuo, I.N.; Onabanjo, A.O. The in vitro and in vivo effects of mefloquine on Trypanosoma brucei brucei. J. Hyg. Epidemiol. Microbiol. Immunol. 1992, 36, 191–199. [Google Scholar] [PubMed]
  81. Planer, J.D.; Hulverson, M.A.; Arif, J.A.; Ranade, R.M.; Don, R.; Buckner, F.S. Synergy testing of fda-approved drugs identifies potent drug combinations against Trypanosoma cruzi. PLoS Negl. Trop. Dis. 2014, 8, e2977. [Google Scholar] [CrossRef] [PubMed]
  82. Lasserre, R. Treatment of amoebiasis. Phil. J. Microbiol. Infect. Dis. 1979, 8, 1. [Google Scholar]
  83. Lemmens-Gruber, R.; Karkhaneh, A.; Studenik, C.; Heistracher, P. Cardiotoxicity of emetine dihydrochloride by calcium channel blockade in isolated preparations and ventricular myocytes of guinea-pig hearts. Br. J. Pharmacol. 1996, 117, 377–383. [Google Scholar] [CrossRef]
  84. Matthews, H.; Usman-Idris, M.; Khan, F.; Read, M.; Nirmalan, N. Drug repositioning as a route to anti-malarial drug discovery: Preliminary investigation of the in vitro anti-malarial efficacy of emetine dihydrochloride hydrate. Malaria J. 2013, 12, 359. [Google Scholar] [CrossRef] [PubMed]
  85. Jones, D.C.; Hallyburton, I.; Stojanovski, L.; Read, K.D.; Frearson, J.A.; Fairlamb, A.H. Identification of a kappa-opioid agonist as a potent and selective lead for drug development against human african trypanosomiasis. Biochem. Pharmacol. 2010, 80, 1478–1486. [Google Scholar] [CrossRef] [PubMed]
  86. Chan, S.L.; Lee, M.C.; Tan, K.O.; Yang, L.K.; Lee, A.S.; Flotow, H.; Fu, N.Y.; Butler, M.S.; Soejarto, D.D.; Buss, A.D.; et al. Identification of chelerythrine as an inhibitor of bclxl function. J. Biol. Chem. 2003, 278, 20453–20456. [Google Scholar] [CrossRef] [PubMed]
  87. Rosenkranz, V.; Wink, M. Alkaloids induce programmed cell death in bloodstream forms of trypanosomes (trypanosoma b. Brucei). Molecules 2008, 13, 2462–2473. [Google Scholar] [CrossRef] [PubMed]
  88. Duffy, S.; Sykes, M.L.; Jones, A.J.; Shelper, T.B.; Simpson, M.; Lang, R.; Poulsen, S.A.; Sleebs, B.E.; Avery, V.M. Screening the mmv pathogen box across multiple pathogens reclassifies starting points for open source drug discovery. Antimicrob. Agents Chemother. 2017, 61, 9. [Google Scholar] [CrossRef] [PubMed]
  89. Davis, R.A. Isolation and structure elucidation of the new fungal metabolite (−)-xylariamide a. J. Nat. Prod. 2005, 68, 769–772. [Google Scholar] [CrossRef] [PubMed]
  90. Levrier, C.; Balastrier, M.; Beattie, K.D.; Carroll, A.R.; Martin, F.; Choomuenwai, V.; Davis, R.A. Pyridocoumarin, aristolactam and aporphine alkaloids from the Australian rainforest plant Goniothalamus australis. Phytochemistry 2013, 86, 121–126. [Google Scholar] [CrossRef] [PubMed]
  91. Choomuenwai, V.; Andrews, K.T.; Davis, R.A. Synthesis and antimalarial evaluation of a screening library based on a tetrahydroanthraquinone natural product scaffold. Bioorg. Med. Chem. 2012, 20, 7167–7174. [Google Scholar] [CrossRef] [PubMed]
  92. Barnes, E.C.; Said, N.A.B.; Williams, E.D.; Hooper, J.N.; Davis, R.A. Ecionines A and B, two new cytotoxic pyridoacridine alkaloids from the Australian marine sponge, Ecionemia geodides. Tetrahedron 2010, 66, 283–287. [Google Scholar] [CrossRef]
  93. Barnes, E.C.; Kumar, R.; Davis, R.A. The use of isolated natural products as scaffolds for the generation of chemically diverse screening libraries for drug discovery. Nat. Prod. Rep. 2016, 33, 372–381. [Google Scholar] [CrossRef] [PubMed]
Sample Availability: Samples of the compounds are not available from the authors.
Figure 1. Structures of compounds 115.
Figure 1. Structures of compounds 115.
Molecules 22 01715 g001
Figure 2. (A) Spider plot displaying the IC50 values of compounds for L. donovani DD8 promastigotes, L. donovani DD8 intracellular amastigotes, T. b. brucei and T. cruzi; (B) Venn diagram for overlapping active hits identified against L. donovani DD8 promastigotes, L. donovani DD8 intracellular amastigotes, T. b. brucei and T. cruzi.
Figure 2. (A) Spider plot displaying the IC50 values of compounds for L. donovani DD8 promastigotes, L. donovani DD8 intracellular amastigotes, T. b. brucei and T. cruzi; (B) Venn diagram for overlapping active hits identified against L. donovani DD8 promastigotes, L. donovani DD8 intracellular amastigotes, T. b. brucei and T. cruzi.
Molecules 22 01715 g002
Figure 3. Scatter plot of active hits against L. donovani DD8 promastigotes and L. donovani DD8 intracellular amastigotes. Right top quadrant exhibiting compounds found to be active on both L. donovani DD8 promastigotes and L. donovani DD8 intracellular amastigotes.
Figure 3. Scatter plot of active hits against L. donovani DD8 promastigotes and L. donovani DD8 intracellular amastigotes. Right top quadrant exhibiting compounds found to be active on both L. donovani DD8 promastigotes and L. donovani DD8 intracellular amastigotes.
Molecules 22 01715 g003
Table 1. Dose response and mechanism of action of reference compounds against L. donovani DD8, T. b. brucei and T. cruzi.
Table 1. Dose response and mechanism of action of reference compounds against L. donovani DD8, T. b. brucei and T. cruzi.
CompoundL. donovani DD8 PromastigotesL. donovani DD8 Intracellular AmastigotesT. b. bruceiT. cruziMechanism of Action
IC50 (mean ± SD) (μM)
(S.I HEK-293)
(S.I THP-1)
IC50 (mean ± SD) (μM)
(S.I HEK-293)
(S.I THP-1)
IC50 (mean ± SD) (μM)
(S.I HEK-293)
IC50 (mean ± SD) (μM)
(S.I HEK-293)
Amphotericin B0.12 ± 0.01
(>68.27) a (>16.39) b
0.20 ± 0.02
(>41.65) a (>9.61) b
--Binds to ergosterol, the principal sterol in fungal cell membranes and Leishmania cells [41].
Miltefosine3.48 ± 0.26
(>9.57) a (>5.74) b
2.54 ± 0.57
(>13.12) a (>7.27) b
--Interacts with lipids (phospholipids and sterols), including membrane lipids, inhibition of cytochrome C oxidase (mitochondrial function), and apoptosis [42,43].
Pentamidine-
-0.002 ± 0.001
(>334.52) a
-Accumulates in trypanosomes; disrupts mitochondrial processes [44].
Diminazene-
-0.04 ± 0.01
(>962.75) a
-Interferes with RNA editing and trans-splicing [44].
Puromycin-
-0.03 ± 0.00
(12.96) a
1.65 ± 0.35
(0.150) a
Protein synthesis inhibitor via premature chain termination during translation in the ribosome [44].
Benznidazole-
--3.36 ± 1.52
(>44.84) a
Causes oxidation in the nucleotide pool which in turn causes the formation of breaks in double stranded DNA [45].
Nifurtimox---0.62 ± 0.10
(> 243.03) a
Unknown. Possibly appears to be due to oxidative stress—potentially from the formation of hydrogen peroxide [46].
All results from two independent experiments (n = 2). a Selectivity index (SI) compared to HEK-293 cells. b Selectivity index (SI) compared to THP-1 cells.
Table 2. In vitro anti-kinetoplastid activities of compounds.
Table 2. In vitro anti-kinetoplastid activities of compounds.
CompoundsAnti-Leishmanial ActivityAnti-Trypanosomal ActivityComments
L. donovani DD8 PromastigotesL. donovani DD8 Intracellular AmastigotesT. b. bruceiT. cruzi
IC50 (mean ± SD) (μM)IC50 (mean ± SD) (μM)IC50 (mean ± SD) (μM)IC50 (mean ± SD) (μM)
Lissoclinotoxin E (1)0.72 ± 0.16
(>11.72) a (6.88) b
4.41 ± 0.24
(>2.26) a (1.13) b
0.57 ± 0.20
(>17.68) a
3.92 ± 0.38
(>2.52) a(>4.67) c
Active on all three parasites with IC50 < 5 uM. Exhibits high selectivity for T. b. brucei in relation to HEK-293 cells.
Spermatinamine (2)11.87 ± 0.56
(0.33) a (0.41) b
6.15 ± 0.05
(0.64) a (0.80) b
1.00 ± 0.26
(5.89) a
-Active on L. donovani DD8 and T. b. brucei. Moderate selectivity for T. b. brucei in relation to HEK-293 cells.
Thiaplakortone A (3)--3.94 ± 0.78
(0.70) a
4.26 ± 0.65
(0.53) a (1.06) c
Active on T. b. brucei and T. cruzi with no selectivity.
Gambogic acid (4)100% inhibition at
16.7 µM
-0.27 ± 0.04
(7.11) a
1.87 ± 0.07
(1.50) a (1.00) c
Active on T .b. brucei and T. cruzi. Moderately selective for T. b. brucei in relation to HEK 293 cells.
Mycophenolic acid (5)--0.51 ± 0.10
(1.01) a
1.59 ± 0.03
(0.37) a (0.13) c
Active on T. b. brucei and T. cruzi with no selectivity.
Narciclasine (6)100% inhibition at
16.7 µM
-0.03 ± 0.01
(1.09) a
0.20 ± 0.01
(0.21) a (3.28) c
Active on T. b. brucei and T. cruzi. Low selectivity for T. cruzi in 3T3 cells.
Thiaplakortone analogue (7)--0.68 ± 0.01
(4.19) a
3.55 ± 0.38
(1.46) a (1.33) c
Active on T. b. brucei and T. cruzi. Moderately selective for T. b. brucei in relation to HEK-293 cells.
Mefloquine HCl (8)100% inhibition at
16.7 µM
-0.62 ± 0.06
(7.97) a
3.96 ± 0.58
(>5.06) a (>1.70) c
Moderate selectivity for T. cruzi and T. b. brucei in relation to HEK 293 cells.
3,4-Dihydro-2H-naphtho[2,3-b]-[1,4]
thiazine-5,10-dione 1,1-dioxide (9)
100% inhibition at
16.7 µM
--3.81 ± 0.60
(1.11) a (1.24) c
Active on L. donovani DD8 promastigotes and T. cruzi with no selectivity.
Emetine dihydrochloride (10)100% inhibition at
16.7 µM
-0.05 ± 0.01
(1.61) a
0.09 ± 0.00
(0.92) a (6.04) c
Active on T. b. brucei and T. cruzi with selectivity for T. cruzi in 3T3 cells.
Lissoclinotoxin F (11)5.51 ± 0.30
(>3.62) a (>1.81) b
8.31 ± 0.67
(>2.40) a (>1.20) b
--Active on both forms of L. donovani DD8 parasite. Low selectivity for both forms of L. donovani DD8 parasite.
Ethyl 4-((diethyl-amino)methyl)-5-hydroxy-1-(4-methoxyphenyl)-2-methyl-1H-indole-3-carboxylate (12)100% inhibition at
16.7 µM
11.09 ± 0.31
(>2.97) a (>1.50) b
--Low selectivity for L. donovani DD8 intracellular amastigotes.
Chalcone analogue (13)100% inhibition at
16.7 µM
5.65 ± 0.26
(11.87) a (3.59) b
--Active on L. donovani DD8 intracellular amastigotes. Highly selective for L. donovani DD8 intracellular amastigotes in relation to HEK 293 cells.
Psammaplysin F (14)---5.63 ± 0.76
(>3.51) a (>1.90) c
Moderate Selectivity for T. cruzi in relation to
HEK-293 cells.
Chelerythrine chloride (15)--0.23 ± 0.04
(>18.71) a
-Only selective on T. b. brucei. Highly selective for T. b. brucei in relation to HEK-293 cells.
All results from two independent experiments (n = 2). a Selectivity index (SI) compared to HEK-293 cells. b Selectivity index (SI) compared to THP-1 cells. c Selectivity index (SI) compared to 3T3 cells.

Share and Cite

MDPI and ACS Style

Zulfiqar, B.; Jones, A.J.; Sykes, M.L.; Shelper, T.B.; Davis, R.A.; Avery, V.M. Screening a Natural Product-Based Library against Kinetoplastid Parasites. Molecules 2017, 22, 1715. https://doi.org/10.3390/molecules22101715

AMA Style

Zulfiqar B, Jones AJ, Sykes ML, Shelper TB, Davis RA, Avery VM. Screening a Natural Product-Based Library against Kinetoplastid Parasites. Molecules. 2017; 22(10):1715. https://doi.org/10.3390/molecules22101715

Chicago/Turabian Style

Zulfiqar, Bilal, Amy J. Jones, Melissa L. Sykes, Todd B. Shelper, Rohan A. Davis, and Vicky M. Avery. 2017. "Screening a Natural Product-Based Library against Kinetoplastid Parasites" Molecules 22, no. 10: 1715. https://doi.org/10.3390/molecules22101715

Article Metrics

Back to TopTop