Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides
AbstractSilica nanoparticles were functionalized with immobilized molecular bait, Cibacron Blue, and a porous polymeric bis-acrylamide shell. These nanoparticles represent a new alternative to capture low molecular weight (LMW) proteins/peptides, that might be potential biomarkers. Functionalized core-shell silica nanoparticles (FCSNP) presented a size distribution of 243.9 ± 11.6 nm and an estimated surface charge of −38.1 ± 0.9 mV. The successful attachment of compounds at every stage of synthesis was evidenced by ATR-FTIR. The capture of model peptides was determined by mass spectrometry, indicating that only the peptide with a long sequence of hydrophobic amino acids (alpha zein 34-mer) interacted with the molecular bait. FCSNP excluded the high molecular weight protein (HMW), BSA, and captured LMW proteins (myoglobin and aprotinin), as evidenced by SDS-PAGE. Functionalization of nanoparticles with Cibacron Blue was crucial to capture these molecules. FCSNP were stable after twelve months of storage and maintained a capacity of 3.1–3.4 µg/mg. View Full-Text
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Hernandez-Leon, S.G.; Sarabia-Sainz, J.A.-I.; Montfort, G.R.-C.; Guzman-Partida, A.M.; Robles-Burgueño, M.R.; Vazquez-Moreno, L. Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides. Molecules 2017, 22, 1712.
Hernandez-Leon SG, Sarabia-Sainz JA-I, Montfort GR-C, Guzman-Partida AM, Robles-Burgueño MR, Vazquez-Moreno L. Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides. Molecules. 2017; 22(10):1712.Chicago/Turabian Style
Hernandez-Leon, Sergio G.; Sarabia-Sainz, Jose A.-I.; Montfort, Gabriela R.-C.; Guzman-Partida, Ana M.; Robles-Burgueño, Maria R.; Vazquez-Moreno, Luz. 2017. "Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides." Molecules 22, no. 10: 1712.
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