Next Article in Journal
Facile and Green Synthesis of Saturated Cyclic Amines
Previous Article in Journal
Effects of Sorghum Malting on Colour, Major Classes of Phenolics and Individual Anthocyanins
Previous Article in Special Issue
Biomimetic-Functionalized, Tannic Acid-Templated Mesoporous Silica as a New Support for Immobilization of NHase
Article Menu
Issue 10 (October) cover image

Export Article

Open AccessArticle
Molecules 2017, 22(10), 1712; doi:10.3390/molecules22101712

Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides

1
Centro de Investigación en Alimentación y Desarrollo A.C. Carretera a la Victoria km 0.6 C.P. 83304, Hermosillo 83304, Sonora, Mexico
2
Universidad de Sonora. Blvd. Luis Encinas y Rosales S/N, Col. Centro, Hermosillo 83000, Sonora, Mexico
*
Author to whom correspondence should be addressed.
Received: 7 September 2017 / Revised: 6 October 2017 / Accepted: 9 October 2017 / Published: 12 October 2017
(This article belongs to the Special Issue Mesoporous Silica in Biomedical Applications)
View Full-Text   |   Download PDF [3823 KB, uploaded 12 October 2017]   |  

Abstract

Silica nanoparticles were functionalized with immobilized molecular bait, Cibacron Blue, and a porous polymeric bis-acrylamide shell. These nanoparticles represent a new alternative to capture low molecular weight (LMW) proteins/peptides, that might be potential biomarkers. Functionalized core-shell silica nanoparticles (FCSNP) presented a size distribution of 243.9 ± 11.6 nm and an estimated surface charge of −38.1 ± 0.9 mV. The successful attachment of compounds at every stage of synthesis was evidenced by ATR-FTIR. The capture of model peptides was determined by mass spectrometry, indicating that only the peptide with a long sequence of hydrophobic amino acids (alpha zein 34-mer) interacted with the molecular bait. FCSNP excluded the high molecular weight protein (HMW), BSA, and captured LMW proteins (myoglobin and aprotinin), as evidenced by SDS-PAGE. Functionalization of nanoparticles with Cibacron Blue was crucial to capture these molecules. FCSNP were stable after twelve months of storage and maintained a capacity of 3.1–3.4 µg/mg. View Full-Text
Keywords: functionalized core-shell silica nanoparticles; Cibacron Blue; low molecular weight proteins/peptides; high molecular weight proteins; hydrophobic functionalized core-shell silica nanoparticles; Cibacron Blue; low molecular weight proteins/peptides; high molecular weight proteins; hydrophobic
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hernandez-Leon, S.G.; Sarabia-Sainz, J.A.-I.; Montfort, G.R.-C.; Guzman-Partida, A.M.; Robles-Burgueño, M.R.; Vazquez-Moreno, L. Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides. Molecules 2017, 22, 1712.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top