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Molecules 2017, 22(1), 44; doi:10.3390/molecules22010044

Isolation of CHS Gene from Brunfelsia acuminata Flowers and Its Regulation in Anthocyanin Biosysthesis

College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
These authors contributed equally to this paper.
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Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 17 November 2016 / Revised: 23 December 2016 / Accepted: 25 December 2016 / Published: 29 December 2016
(This article belongs to the Section Bioorganic Chemistry)
View Full-Text   |   Download PDF [8541 KB, uploaded 29 December 2016]   |  

Abstract

Chalcone synthase gene (BaCHS) from Brunfelsia acuminata flowers was isolated using RT-PCR and RACE. The coding region of the gene is 1425-bp with an open reading frame of 1170-bp, 73-bp 5′UTR, and 172-bp 3′UTR. Its deduced protein does not have a signal peptide but does contain a cond_enzyme superfamily domain, and consists of 389 amino acids with a predicted molecular mass of 42,699 Da and a pI of 6.57. The deduced amino acid sequence of BaCHS shares 90%, 88%, 85%, 84% and 79% identity with CHS from Petunia hybrida, Nicotiana tabacum, Solanum lycopersicum, Capsicum annuum and Camellia sinensis, respectively. The striking color change from dark purple to light purple and ultimately lead to pure white resulted from a decline in anthocyanin content of the petals and was preceded by a decrease in the expression of BaCHS. Its gene expression was positively correlated with the contents of anthocyanin (p ≤ 0.01). View Full-Text
Keywords: Brunfelsia acuminata; flower color; anthocyanin biosynthesis; chalcone synthase (CHS); RT-qPCR Brunfelsia acuminata; flower color; anthocyanin biosynthesis; chalcone synthase (CHS); RT-qPCR
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MDPI and ACS Style

Li, M.; Cao, Y.-T.; Ye, S.-R.; Irshad, M.; Pan, T.-F.; Qiu, D.-L. Isolation of CHS Gene from Brunfelsia acuminata Flowers and Its Regulation in Anthocyanin Biosysthesis. Molecules 2017, 22, 44.

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