Next Article in Journal
Three Novel Triterpenoids from Taraxacum officinale Roots
Next Article in Special Issue
The Draft Genome Sequence of Actinokineospora bangkokensis 44EHWT Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units
Previous Article in Journal
Anti-Influenza Virus Activity and Constituents Characterization of Paeonia delavayi Extracts
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle
Molecules 2016, 21(9), 1131; doi:10.3390/molecules21091131

New Deferoxamine Glycoconjugates Produced upon Overexpression of Pathway-Specific Regulatory Gene in the Marine Sponge-Derived Streptomyces albus PVA94-07

1
Department of Pharmacognosy, University of Vienna, 1090 Vienna, Austria
2
Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, 18016 Armilla, Granada, Spain
3
Department of Biotechnology, SINTEF Materials and Chemistry, N-7465 Trondheim, Norway
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 1 July 2016 / Revised: 22 August 2016 / Accepted: 24 August 2016 / Published: 27 August 2016
(This article belongs to the Special Issue Genomics-based Discovery of Microbial Natural Products)
View Full-Text   |   Download PDF [1016 KB, uploaded 27 August 2016]   |  

Abstract

Activation of silent biosynthetic gene clusters in Streptomyces bacteria via overexpression of cluster-specific regulatory genes is a promising strategy for the discovery of novel bioactive secondary metabolites. This approach was used in an attempt to activate a cryptic gene cluster in a marine sponge-derived Streptomyces albus PVA94-07 presumably governing the biosynthesis of peptide-based secondary metabolites. While no new peptide-based metabolites were detected in the recombinant strain, it was shown to produce at least four new analogues of deferoxamine with additional acyl and sugar moieties, for which chemical structures were fully elucidated. Biological activity tests of two of the new deferoxamine analogues revealed weak activity against Escherichia coli. The gene knockout experiment in the gene cluster targeted for activation, as well as overexpression of certain genes from this cluster did not have an effect on the production of these compounds by the strain overexpressing the regulator. It seems plausible that the production of such compounds is a response to stress imposed by the production of an as-yet unidentified metabolite specified by the cryptic cluster. View Full-Text
Keywords: Streptomyces; secondary metabolite biosynthesis; gene cluster; regulatory gene; deferoxamine; glycoconjugates Streptomyces; secondary metabolite biosynthesis; gene cluster; regulatory gene; deferoxamine; glycoconjugates
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Sekurova, O.N.; Pérez-Victoria, I.; Martín, J.; Degnes, K.F.; Sletta, H.; Reyes, F.; Zotchev, S.B. New Deferoxamine Glycoconjugates Produced upon Overexpression of Pathway-Specific Regulatory Gene in the Marine Sponge-Derived Streptomyces albus PVA94-07. Molecules 2016, 21, 1131.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top