Next Article in Journal
Simultaneous Ultra Performance Liquid Chromatography Determination and Antioxidant Activity of Linarin, Luteolin, Chlorogenic Acid and Apigenin in Different Parts of Compositae Species
Previous Article in Journal
AutoDock-GIST: Incorporating Thermodynamics of Active-Site Water into Scoring Function for Accurate Protein-Ligand Docking
Previous Article in Special Issue
New Deferoxamine Glycoconjugates Produced upon Overexpression of Pathway-Specific Regulatory Gene in the Marine Sponge-Derived Streptomyces albus PVA94-07
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Molecules 2016, 21(11), 1607; doi:10.3390/molecules21111607

The Draft Genome Sequence of Actinokineospora bangkokensis 44EHWT Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units

1
Department of Pharmaceutical Biology and Biotechnology, Institute of Pharmaceutical Sciences, Albert-Ludwigs-University of Freiburg, Stefan-Meier-Straße 19, 79104 Freiburg, Germany
2
Department of Biotechnology, Faculty of Science, Mahidol University, 272 Rama 6 Road, Bangkok 10400, Thailand
3
Mahidol University and Osaka University Collaborative Research Center for Bioscience and Biotechnology, Bangkok 10400, Thailand
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 17 October 2016 / Revised: 17 November 2016 / Accepted: 19 November 2016 / Published: 23 November 2016
(This article belongs to the Special Issue Genomics-based Discovery of Microbial Natural Products)
View Full-Text   |   Download PDF [2426 KB, uploaded 23 November 2016]   |  

Abstract

We report the draft genome sequence of Actinokineospora bangkokensis 44EHWT, the producer of the antifungal polyene compounds, thailandins A and B. The sequence contains 7.45 Mb, 74.1% GC content and 35 putative gene clusters for the biosynthesis of secondary metabolites. There are three gene clusters encoding large polyketide synthases of type I. Annotation of the ORF functions and targeted gene disruption enabled us to identify the cluster for thailandin biosynthesis. We propose a plausible biosynthetic pathway for thailandin, where the unusual butylmalonyl-CoA extender unit is incorporated and results in an untypical side chain. View Full-Text
Keywords: Actinokineospora bangkokensis; thailandin; polyene; biosynthetic gene cluster; butylmalonyl-CoA; draft genome sequence; genome mining Actinokineospora bangkokensis; thailandin; polyene; biosynthetic gene cluster; butylmalonyl-CoA; draft genome sequence; genome mining
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Greule, A.; Intra, B.; Flemming, S.; Rommel, M.G.E.; Panbangred, W.; Bechthold, A. The Draft Genome Sequence of Actinokineospora bangkokensis 44EHWT Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units. Molecules 2016, 21, 1607.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top